Results: 3

1.
Fig. 2.

Fig. 2. From: E2A proteins maintain the hematopoietic stem cell pool and promote the maturation of myelolymphoid and myeloerythroid progenitors.

Effects of E2A deletion on the numbers of myeloerythroid progenitors in adult mouse BM. Total BM cells from wild-type, E2A +/−, and E2A −/− mice were harvested and prepared for analysis by flow cytometry. (A) Representative staining profiles for myeloerythroid progenitors. (B) Reduced numbers of myeloerythroid progenitor subsets in the BM of E2A −/− mice. Shown are the absolute numbers of the myeloerythroid progenitor subsets in the BM of wild-type, E2A +/−, and E2A −/− mice (n = 5). Horizontal bars show the mean values. Statistical significance determined by unpaired t test, 2-tailed, between E2A −/− and wild type.

Craig L. Semerad, et al. Proc Natl Acad Sci U S A. 2009 February 10;106(6):1930-1935.
2.
Fig. 1.

Fig. 1. From: E2A proteins maintain the hematopoietic stem cell pool and promote the maturation of myelolymphoid and myeloerythroid progenitors.

Effects of E2A deletion on the numbers of HSCs in adult mouse bone marrow (BM). Total BM cells from wild-type, E2A +/−, and E2A −/− mice were harvested and prepared for analysis by flow cytometry. (A) Representative staining profiles for LSK cells and the LT-HSC, ST-HSC, and MPP subpopulations. The small gate in the MPP quadrant is representative of the LMPP population. (B) Reduced HSC numbers in the BM of E2A −/− mice. Shown are the absolute numbers of the LSK, LT-HSC, ST-HSC, and MPP populations in the BM of wild-type, E2A +/−, and E2A −/− mice (n = 6). Horizontal bars show the mean values. Statistical significance determined by unpaired t test, 2-tailed, between E2A −/− and wild type.

Craig L. Semerad, et al. Proc Natl Acad Sci U S A. 2009 February 10;106(6):1930-1935.
3.
Fig. 3.

Fig. 3. From: E2A proteins maintain the hematopoietic stem cell pool and promote the maturation of myelolymphoid and myeloerythroid progenitors.

Increased cycling by E2A-deficient HSCs. To investigate cycling of E2A −/− HSCs, BrdU was administered to 6 week-old wild-type and E2A −/− mice and BrdU incorporation by the LT-HSC, ST-HSC, and MPP cell fractions was analyzed by flow cytometry. (A) Representative staining profiles for the LT-HSC (LSK/CD150+/Flk2) fraction. (B) Increased incorporation of BrdU by E2A −/− HSCs. Shown are the percentages of BrdU incorporation by the LT-HSC (LSK/CD150+/Flk2), ST-HSC (LSK/CD150/Flk2), and MPP (LSK/CD150/Flk2+) fractions in the bone marrow of wild-type and E2A −/− mice (n = 4). Statistical significance determined by unpaired t test, 2-tailed, between E2A −/− and wild type. (C) Cell cycle distribution in E2A −/− HSCs. Shown are the percentages of LSKFlk2+ and LSKFlk2 fractions in G0, G1, and SG2M in the BM of wild-type and E2A −/− mice (n = 3). Statistical significance determined by 2-sided Student's t test, E2A −/−, compared with wild-type. (D) Schematic representation depicting the roles of E2A in early hematopoiesis. The blue arrow indicates the importance of E2A proteins in HSC self-renewal. Decreases in the indicated hematopoietic populations detected in E2A −/− mice are shown by solid red down arrows. In the absence of E2A, significant decreases in LMPPs, CLPs, and GMPs are detected. Also, the Pre MegE, Pre CFU-E, and MkP compartments are significantly decreased. However, E2A −/− mice have near wild-type levels of Pre GMs and CFU-Es. We propose that the E2A proteins act to promote the development of the LMPPs and the Pre MegE progenitors, and to suppress the development of the Pre GM stage into CFU-Es. Data represent the mean ± SD. Statistical significance determined by unpaired t test, 2-tailed, between E2A −/− and wild type.

Craig L. Semerad, et al. Proc Natl Acad Sci U S A. 2009 February 10;106(6):1930-1935.

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