Results: 5

1.
Figure 4

Figure 4. DAPI and MitoTracker images from paclitaxel-treated MCF-7 cells. From: Light Scattering Measurements of Subcellular Structure Provide Noninvasive Early Detection of Chemotherapy-induced Apoptosis.

Representative images from DAPI (a,b) and MitoTracker (c,d) stained control (a,c) and paclitaxel-treated (b,d) MCF-7 cells Scale bars = 10 μm.

Kevin J. Chalut, et al. Cancer Res. ;69(3):1199-1204.
2.
Figure 2

Figure 2. Paclitaxel-treated MCF-7 cells show significant DNA fragmentation within 6. From: Light Scattering Measurements of Subcellular Structure Provide Noninvasive Early Detection of Chemotherapy-induced Apoptosis.

Control and 5 nM paclitaxel-treated MCF-7 cells (3, 6, 9 and 24 hours) were stained with propidium iodide and analyzed by flow cytometry for DNA content, data shown is only the percentage of the cellular population with sub-G1 DNA content. ** indicates high statistical significance (p< 0.001).

Kevin J. Chalut, et al. Cancer Res. ;69(3):1199-1204.
3.
Figure 5

Figure 5. Paclitaxel induces a significant change in the box FD of MCF-7 cell nuclei and mitochondria. From: Light Scattering Measurements of Subcellular Structure Provide Noninvasive Early Detection of Chemotherapy-induced Apoptosis.

(a) nuclei and (b) mitochondria after treatment with 5 nM Paclitaxel for 3, 6, 12, and 24 hours or 3, 6, and 12 hours, respectively. For nuclei, box FD is assessed by QIA of DAPI-stained images using fluorescence microscopy. For mitochondria, box FD is assessed by QIA of MitoTracker FM Green-stained images using confocal fluorescence microscopy.

Kevin J. Chalut, et al. Cancer Res. ;69(3):1199-1204.
4.
Figure. 3

Figure. 3. Paclitaxel and doxorubicin induce a significant change in the mass FD of MCF-7 cells. From: Light Scattering Measurements of Subcellular Structure Provide Noninvasive Early Detection of Chemotherapy-induced Apoptosis.

Summary of mass FD results for MCF-7 cells treated with (a) 5 nM Paclitaxel and (b) 5 μM Doxorubicin at t = 3,6,12 and 24 h post treatment. (c) Comparison of cells treated with 5 nM Paclitaxel and controls at t = 1.5 h. 50 nM Paclitaxel treatment results are shown in supplementary Fig. S3. * indicates statistical significance (p < 0.05) and ** indicates high statistical significance (p < 0.001).

Kevin J. Chalut, et al. Cancer Res. ;69(3):1199-1204.
5.
Figure 1

Figure 1. Paclitaxel induces apoptosis and G2/M arrest in MCF-7 breast cancer cells. From: Light Scattering Measurements of Subcellular Structure Provide Noninvasive Early Detection of Chemotherapy-induced Apoptosis.

a) Control MCF-7 cells and cells treated for 24 hours with 1, 5, 10, 25, and 50 nM paclitaxel were fixed and stained with propidium iodide and then analyzed by flow cytometry for cellular DNA content. Results shown in Figure 1a are only the sub-G1 and G2/M (4n) DNA content populations. b) Complete cell cycle analysis from control MCF-7 cells and cells treated with 5 nM and 50 nM paclitaxel for 24 hours.

Kevin J. Chalut, et al. Cancer Res. ;69(3):1199-1204.

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