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1.
Figure 4

Figure 4. From: Rapid microglial response around amyloid pathology following systemic anti-A? antibody administration in PDAPP mice.

Peripheral m3D6 administration results in marked morphological changes in microglia. 3-D reconstructed z-series stack images taken of 22 month old PDAPP+/−; CX3CR1/GFP+/− mice injected with 500 μg of m3D6 (A–C), an anti-Aβ antibody, or not injected (D–F). GFP labeled microglia are green. Fibrillar amyloid was labeled with Methoxy-X04 (blue). Scale bar is 20 μm.

Jessica Koenigsknecht-Talboo, et al. J Neurosci. ;28(52):14156-14164.
2.
Figure 3

Figure 3. From: Rapid microglial response around amyloid pathology following systemic anti-A? antibody administration in PDAPP mice.

Decrease in microglial process movement in older PDAPP mice. The number of microglial process (A) extensions and (B) retractions were counted in young (3 ½ – 6 ½ month) and old (14–17 month) PDAPP+/−;CX3CR1/GFP+/− mice near and distant from amyloid pathology. Five mice were studied per age group and 6 fields of view were imaged in each animal. Data is presented as mean +/− SEM. *p<0.001.

Jessica Koenigsknecht-Talboo, et al. J Neurosci. ;28(52):14156-14164.
3.
Figure 1

Figure 1. From: Rapid microglial response around amyloid pathology following systemic anti-A? antibody administration in PDAPP mice.

Microglia have altered morphology and cluster around amyloid plaques in PDAPP+/−;CX3CR1/GFP+/− mice. 3-D reconstructed z-series stack images taken of cortical microglia in (A) PDAPP+/−;CX3CR1/GFP+/− mice at 3 ½ months of age (in the absence of plaques), in (B) 14 month old PDAPP+/−;CX3CR1/GFP+/− mice around amyloid plaques (blue), in (C) 14 month old PDAPP+/−;CX3CR1/GFP+/− mice in areas lacking plaques, and in (D) 5 month old PDAPP−/−;CX3CR1/GFP+/− mice. GFP labeled microglia are green. Vessels are labeled with Texas-Red dextran. Amyloid fluoresces blue after injection of Methoxy-X04. Scale bar is 20 μm.

Jessica Koenigsknecht-Talboo, et al. J Neurosci. ;28(52):14156-14164.
4.
Figure 6

Figure 6. From: Rapid microglial response around amyloid pathology following systemic anti-A? antibody administration in PDAPP mice.

CD45 colocalizes with GFP-labeled microglia around amyloid pathology and there is increased staining around plaques following m3D6 administration. Brain sections from 18 month old PDAPP+/−;CX3CR1/GFP+/− mice were stained with CD45 and images were taken at low power (A–H) and high power (I–P). CD45 positive cells are in red (A, E, I, M), GFP labels CX3CR1 positive microglia (B, F, J, N), and Methoxy-X04 labels aggregated amyloid in blue (C, G, K, O). Merged images are also shown (D, G, L, P). Scale bars are 100 μm and 50 μm for low and high power images, respectively.

Jessica Koenigsknecht-Talboo, et al. J Neurosci. ;28(52):14156-14164.
5.
Figure 5

Figure 5. From: Rapid microglial response around amyloid pathology following systemic anti-A? antibody administration in PDAPP mice.

Iba-1 staining colocalizes with GFP-labeled microglia and there is increased staining around plaques following m3D6 administration. Brain sections from 18 month old PDAPP+/−;CX3CR1/GFP+/− mice were stained with Iba-1 and images were taken at low power (A–H) and high power (I–P). Iba-1 positive cells are in red (A, E, I, M), GFP labels CX3CR1 positive microglia (B, F, J, N), and Methoxy-X04 labels aggregated amyloid in blue (C, G, K, O). Merged images are also shown (D, G, L, P). Scale bars are 100 μm and 50 μm for low and high power images, respectively.

Jessica Koenigsknecht-Talboo, et al. J Neurosci. ;28(52):14156-14164.
6.
Figure 7

Figure 7. From: Rapid microglial response around amyloid pathology following systemic anti-A? antibody administration in PDAPP mice.

Microglial activation after antibody treatment requires recognition of aggregated Aβ and the Fc domain. (A) The number of microglial processes and (B) the number of microglial cells were counted in 3-D reconstructed z-series stack images in 18 month old PDAPP+/−;CX3CR1/GFP+/− mice not injected (untreated) or injected with 500 μg of m3D6, 500 μg of IgG2b, 500 μg of mHJ5.1, 500 μg of m3D6 Fab fragments. Four mice were studied per treatment group and 6–10 fields of view were imaged in each animal. Data is presented as mean +/− SEM. * p<0.001.

Jessica Koenigsknecht-Talboo, et al. J Neurosci. ;28(52):14156-14164.
7.
Figure 2

Figure 2. From: Rapid microglial response around amyloid pathology following systemic anti-A? antibody administration in PDAPP mice.

Decrease in the number of microglial processes around amyloid deposition. The number of processes extending from the cell body were counted in areas containing CAA, parenchymal amyloid plaques, and no amyloid pathology (no path). Microglia were compared in 14–17 month old mice around CAA, parenchymal amyloid plaques, and in areas with no amyloid pathology as well as in 3 ½ – 6 ½ month old PDAPP+/−;CX3CR1/GFP+/− mice lacking amyloid pathology and 5 month old PDAPP−/−;CX3CR1/GFP+/− mice. Five mice were studied per age group in APP transgenic mice and 6 fields of view were imaged in each animal. Six fields of view were studied in 2 non-APP transgenic mice. Data is presented as mean +/− SEM. * p<0.001.

Jessica Koenigsknecht-Talboo, et al. J Neurosci. ;28(52):14156-14164.

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