Results: 5

1.
FIG. 4.

FIG. 4. From: Effect of Leptin on Mouse Trophoblast Giant Cells.

Expression profiles of selected genes by microarray. Columns represent mean normalized expression levels on a log2 scale. Fold changes (LEP/CON) at 24 h on the raw scale are indicated above the columns. Bars represent standard error. a) Trophoblast-specific genes. b) Genes negatively associated with polyploidy. c) Genes positively associated with polyploidy. d) Apoptosis-related genes.

L.C. Schulz, et al. Biol Reprod. 2009 March;80(3):415-424.
2.
FIG. 2.

FIG. 2. From: Effect of Leptin on Mouse Trophoblast Giant Cells.

Gelatin zymography of trophoblast cell culture medium. There are two major bands of gelatinocytic activity at approximately 92 kDa, the size of pro-matrix metalloproteinase 9 (proMMP9), and at approximately 65 kDa, the size of active matrix metalloproteinase 2 (MMP2). There are faint bands at 86 kDa, the size of active MMP9, and 72 kDa, the size of pro-MMP2 (proMMP2).

L.C. Schulz, et al. Biol Reprod. 2009 March;80(3):415-424.
3.
FIG. 3.

FIG. 3. From: Effect of Leptin on Mouse Trophoblast Giant Cells.

Effect of MAP2K1 (MEK (U0126) inhibitor and STAT3 (cucurbitacin I) inhibitors on leptin-stimulated metalloproteinase activity. Dimethyl sulfoxide was the vehicle for both inhibitors. The y-axis shows activity of fluorescent gelatinase substrate in sample medium—fluorescence in medium not exposed to cells (bkgd). Metalloproteinase activity was significantly higher in the medium of primary trophoblast cells treated with 50 ng/ml leptin compared with cells cultured in serum-free medium alone. Cells treated with cucurbitacin and leptin had significantly less activity than those treated with leptin and vehicle (DMSO) only. Columns with different letters are significantly different (ANOVA, Tukey test P < 0.05). Bars represent standard error. N = 5.

L.C. Schulz, et al. Biol Reprod. 2009 March;80(3):415-424.
4.
FIG. 5.

FIG. 5. From: Effect of Leptin on Mouse Trophoblast Giant Cells.

Feulgen staining of primary trophoblast cells after plating (control 0) and 24 h later with (leptin 24) or without (control 24) leptin treatment. Representative photographs of control 0 (a), control 24 (b), and leptin 24 (c). Original magnification ×200. df) Average nucleic acid content of cultured trophoblast cells in three independent experiments, determined by multiplying Feulgen stain intensity × area in arbitrary units. g) Frequency distribution showing the number of cells with each nucleic acid content in the control 24 and leptin 24 groups across all three experiments.

L.C. Schulz, et al. Biol Reprod. 2009 March;80(3):415-424.
5.
FIG. 1.

FIG. 1. From: Effect of Leptin on Mouse Trophoblast Giant Cells.

Primary mouse trophoblast cells were treated with 50 ng/ml leptin and lysed at 3, 10, 30, and 60 min after treatment. a) Effect of leptin on phosphorylation of STAT3 at serine 727, as measured by Western blotting. b) Effect of leptin on phosphorylation of STAT3 at tyrosine 705, as measured by ELISA. c) Effect of leptin on SOCS3 expression, as measured by Western blotting. d) Effect of leptin on phosphorylation of MAP2K1 (MEK) at serines 217/221, as measured by ELISA. Protein concentrations are shown relative to those at time 0 for each experiment. Band densities were normalized to actin band densities to control for loading (a and c). P-MAP2K1 (MEK) was normalized to total MAP2K1 (MEK), also measured by ELISA (d). The protein of interest changed significantly over time in all four experiments (repeated-measures ANOVA; P = 0.05). *Significant difference from time 0 (a: Dunnett P < 0.05; bd; Bonferroni P < 0.05). Bars represent standard error. N = 3 (a and c) and N = 7 (b and d).

L.C. Schulz, et al. Biol Reprod. 2009 March;80(3):415-424.

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