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Results: 4

1.
Fig.1

Fig.1. Expression of CB1 receptor and the effect of rimonabant (SR1) on cell death in HCASMCs. From: Cannabinoid CB1 receptor inhibition decreases vascular smooth muscle migration and proliferation.

(A) Western blot reveals expression of CB1 in HCASMCs. Lanes 1-4 indicate lysates prepared from various batches of HCASMCs. (B) Shown are the representative images of scatter plots from three independent experiments demonstrating that SR1 at various concentrations had no and effect on apoptosis/necrosis in HCASMCs determined by flow cytometry.

Mohanraj Rajesh, et al. Biochem Biophys Res Commun. ;377(4):1248-1252.
2.
Fig.2

Fig.2. Effect of rimonabant (SR1) on PDGF-BB induced proliferation of HCASMCs. From: Cannabinoid CB1 receptor inhibition decreases vascular smooth muscle migration and proliferation.

Cells were treated as indicated and the effect of SR1 on PDGF-BB induced proliferation of human vascular smooth muscle cells was determined by measuring the rate of BrdU incorporation employing the commercially available kit. SR1 (5.0 μM) by itself had no any effect of proliferation of HCASMCs. *P< 0.01 vs. control/SR1; #< 0.05 or 0.01 vs. PDGF-BB (20 ng/ml) alone, n=9.

Mohanraj Rajesh, et al. Biochem Biophys Res Commun. ;377(4):1248-1252.
3.
Fig.4

Fig.4. Effect of rimonabant (SR1) on PDGFR-β, Ras and ERK1/2 activation in HCASMCs. From: Cannabinoid CB1 receptor inhibition decreases vascular smooth muscle migration and proliferation.

(A) PDGF-BB activation of PDGFR-β was not inhibited by treatment with SR1. Shown is the representative image of PDGFR-β activation by PDGF-BB (20 ng/ml) for 25 min and the effect of SR1. *P < 0.01 vs. control; n=3. SR1 (5.0μM) by itself had no effect on PDGFR-β phosphorylation. (B) Representative immunoblot depicts the concentration-dependent inhibitory effect of SR1 on PDGF-BB-induced stimulation of Ras activation in smooth muscle cells. *P< 0.01 vs. control; #P< 0.05/0.01 vs. PDGF, n=3. (C) Shown is the representative blot of PDGF-BB-induced activation of ERK1/2 and the concentration-dependent suppression of it by SR1. *P < 0.01 vs. control; # P< 0.05/0.01 vs. PDGF, n=3.

Mohanraj Rajesh, et al. Biochem Biophys Res Commun. ;377(4):1248-1252.
4.
Fig.3

Fig.3. Effect of rimonabant (SR1) on PDGF-BB induced migration of HCASMCs. From: Cannabinoid CB1 receptor inhibition decreases vascular smooth muscle migration and proliferation.

Cells were treated as indicated and migration assays were performed as described in the methods section. (A) Shown are the representative images of smooth muscle cells migrated as per the treatments indicated. (B) Depicts the quantification data for the cells that had migrated in response to the treatment. *P< 0.01 vs. control/SR1; #P < 0.05 or 0.01 vs. PDGF-BB (20 ng/ml) alone, n=6.

Mohanraj Rajesh, et al. Biochem Biophys Res Commun. ;377(4):1248-1252.

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