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4.
Figure 1

Figure 1. Schematic diagram of the metabolic reconstruction and analysis processes.. From: Genome-Scale Reconstruction and Analysis of the Pseudomonas putida KT2440 Metabolic Network Facilitates Applications in Biotechnology.

Solid lines indicate consecutive steps of the reconstruction. Dashed lines represent information transfer. Dotted lines specify planned tasks.

Jacek Puchałka, et al. PLoS Comput Biol. 2008 October;4(10):e1000210.
5.
Figure 6

Figure 6. Mutational strategies for increased PHA production.. From: Genome-Scale Reconstruction and Analysis of the Pseudomonas putida KT2440 Metabolic Network Facilitates Applications in Biotechnology.

This figure highlights 6 strategies suggested by the modified optknock approach for increased production of AcCoA, a precursor for polyhydroxyalkanoates. (A) AcCoA production ranges vs. growth yield of in silico strains developed using the ‘AcCoA production’ strategy. (B) AcCoA pooling versus growth yield of in silico strains developed using the ‘AcCoA pooling’ strategy.

Jacek Puchałka, et al. PLoS Comput Biol. 2008 October;4(10):e1000210.
6.
Figure 4

Figure 4. Comparison of FVA calculations with 13C experimental flux data.. From: Genome-Scale Reconstruction and Analysis of the Pseudomonas putida KT2440 Metabolic Network Facilitates Applications in Biotechnology.

The explanation of color codes is given in the figure. “0*” means that the reaction is not included in the particular metabolic network; double-headed arrows depict reversible reactions, the bigger head shows direction of the positive flux.

Jacek Puchałka, et al. PLoS Comput Biol. 2008 October;4(10):e1000210.

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