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Results: 7

1.
Fig. 5.

Fig. 5. From: A haplotype of human angiotensinogen gene containing -217A increases blood pressure in transgenic mice compared with -217G.

Plasma angiotensin II level. Plasma angiotensin II level is shown in nontransgenic CON and double transgenic mice containing either AG or AA haplotype of the hAGT gene. Values are means ± SE; n = 6. *P < 0.05.

Sudhir Jain, et al. Am J Physiol Regul Integr Comp Physiol. 2008 December;295(6):R1849-R1857.
2.
Fig. 4.

Fig. 4. From: A haplotype of human angiotensinogen gene containing -217A increases blood pressure in transgenic mice compared with -217G.

Immunohistochemical staining of hAGT in liver and kidney of double transgenic mice using mouse anti-hAGT antibody. A–C: expression of hAGT in sections from the liver of transgenic mice containing AA haplotype, AG haplotype, and nontransgenic mice (C57), respectively. D: expression in liver of transgenic mice containing AA haplotype but without treatment with primary antibody. E–H: corresponding results in kidney sections.

Sudhir Jain, et al. Am J Physiol Regul Integr Comp Physiol. 2008 December;295(6):R1849-R1857.
3.
Fig. 1.

Fig. 1. From: A haplotype of human angiotensinogen gene containing -217A increases blood pressure in transgenic mice compared with -217G.

Different haplotypes of the human angiotensinogen (hAGT) gene. The AGT gene can be separated in two haplotypes (AA and AG) based on nucleotide A at −6 and either nucleotide A or G at −217. Nucleotide −217A almost always occurs with −532T, −793A, and −1074T, whereas nucleotide −217G almost always occurs with −532C, −793G, and −1074G in the hAGT gene promoter.

Sudhir Jain, et al. Am J Physiol Regul Integr Comp Physiol. 2008 December;295(6):R1849-R1857.
4.
Fig. 2.

Fig. 2. From: A haplotype of human angiotensinogen gene containing -217A increases blood pressure in transgenic mice compared with -217G.

hAGT mRNA level in the liver (A) and kidney (B) of transgenic mice containing AA and AG haplotype of the hAGT gene. RNA from the liver and kidney of transgenic mice containing either AA or AG haplotype of the hAGT gene was analyzed by QRT-PCR using ABI system. A: liver from AA haplotype had 1.62-fold increased hAGT mRNA compared with AG haplotype. B: kidney from AA haplotype had 1.45-fold increased hAGT mRNA compared with AG haplotype. Values are means ± SE; n = 6. *P < 0.05.

Sudhir Jain, et al. Am J Physiol Regul Integr Comp Physiol. 2008 December;295(6):R1849-R1857.
5.
Fig. 6.

Fig. 6. From: A haplotype of human angiotensinogen gene containing -217A increases blood pressure in transgenic mice compared with -217G.

Expression of mouse and hRen mRNA in kidney of double transgenic mice containing AG and AA haplotype of the hAGT gene. The mouse and human kidney mRNA level in double transgenic animals was determined by QRT-PCR, as described in materials and methods. A: mouse renin mRNA level in kidney of transgenic animals containing AG or AA haplotype is shown by assuming renin level in the kidney of C57BL6 mice as one (marked CON in the figure). B: hRen mRNA level in kidney of transgenic animals containing AG or AA haplotype is shown by assuming renin level in the kidney of PAC (P1 artificial chromosome)-hRen mice as one (marked CON in the figure). Values are means ± SE; n = 6. *P < 0.05.

Sudhir Jain, et al. Am J Physiol Regul Integr Comp Physiol. 2008 December;295(6):R1849-R1857.
6.
Fig. 3.

Fig. 3. From: A haplotype of human angiotensinogen gene containing -217A increases blood pressure in transgenic mice compared with -217G.

hAGT level in plasma of double transgenic mice. Plasma from double transgenic mice containing either AA or AG haplotype of hAGT gene and human renin (hRen) gene was analyzed by mouse albumin and for AGT level, as described in the text. A: Western blot using hAGT antibody. B: Western blot using mouse albumin antibody. Lanes CON (control), AG, and AA denote plasma from human and transgenic animals containing AG and AA haplotype of the hAGT gene. Position of molecular weight markers is shown on the right side. C: quantitation of AGT protein in transgenic animals containing AG and AA haplotype of the hAGT gene. AGT protein level in transgenic mice containing AA haplotype of the hAGT gene was calculated by assuming protein level in transgenic mice containing AG haplotype as one. Values are means ± SE. *P < 0.05.

Sudhir Jain, et al. Am J Physiol Regul Integr Comp Physiol. 2008 December;295(6):R1849-R1857.
7.
Fig. 7.

Fig. 7. From: A haplotype of human angiotensinogen gene containing -217A increases blood pressure in transgenic mice compared with -217G.

Blood pressure in double transgenic mice and effect of losartan. A: 24-h blood pressure of CON and double transgenic mice containing AA and AG haplotype of hAGT gene and hRen gene was measured by telemetry for 4 days. Each bar represents mean from 6 male animals taken over 4 days. B: blood pressure of control and double transgenic mice during daytime (6 AM to 6 PM), nighttime (6 PM to 6 AM), and whole day (24 h) period, as determined by telemetry. C: daytime blood pressure of CON and double transgenic mice containing AA and AG haplotypes of the hAGT gene and hRen gene in the presence (+) and absence (−) of losartan, as determined by tail cuff method. Values are means ± SE; n = 6. *P < 0.05.

Sudhir Jain, et al. Am J Physiol Regul Integr Comp Physiol. 2008 December;295(6):R1849-R1857.

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