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Results: 3

1.
Figure 1

Figure 1. Workflow of activity based proteomics. From: Activity Based Probes as a tool for Functional Proteomic Analysis of Proteases.

Target proteins in the complex proteome are labeled with activity based probes. Labeled proteins are enriched by affinity purification and identified by mass spectrometry.

Marko Fonović, et al. Expert Rev Proteomics. ;5(5):721-730.
2.
Figure 3

Figure 3. Cleavable and ‘’click” chemistry linkers. From: Activity Based Probes as a tool for Functional Proteomic Analysis of Proteases.

A: Reduction cleavage of disulfide and diazo cleavable linkers.
B: Mechanism of ‘’click” chemistry. Cycloaddition of an azide and alkyne functional group is used for the tag attachment after protein labeling.

Marko Fonović, et al. Expert Rev Proteomics. ;5(5):721-730.
3.
Figure 2

Figure 2. Structure and binding of activity based probes. From: Activity Based Probes as a tool for Functional Proteomic Analysis of Proteases.

A: Structural features of two types of activity based probes are shown. Probe MTS-I-94 targets serine proteases. It is composed of phosphonate warhead, a cleavable diazo linker and a biotin tag. DCG-04 is a cysteine protease probe with epoxide warhead.
B: Probes that target serine and cysteine proteases irreversibly and covalently bind to the active site nucleophile (serine or cysteine).

Marko Fonović, et al. Expert Rev Proteomics. ;5(5):721-730.

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