Display Settings:

Items per page
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 6

1.
Figure 4

Figure 4. From: Mll5 contributes to hematopoietic stem cell fitness and homeostasis.

Mll5−/− bone marrow cells show increased susceptibility to myelosuppression. Survival of Mll5−/− and wild-type littermate control mice (21 per group) that received weekly intraperitoneal doses of 5-fluorouracil (5-FU; 200 mg/kg). Mll5−/− mice showed significantly increased sensitivity to 5-FU (log-rank nonparametric test; P < .0001).

Yan Zhang, et al. Blood. 2009 February 12;113(7):1455-1463.
2.
Figure 2

Figure 2. From: Mll5 contributes to hematopoietic stem cell fitness and homeostasis.

Analysis of hematopoiesis using the spleen colony formation assay. (A) Representative spleens of irradiated mice that received 105 bone marrow cells 8 or 12 days before analysis. (B) Graph showing the average number of colonies per spleen at day 8 or day 12 after transplantation. The difference between genotypes in colony number at day 8 was statistically significant by Student t test (P < .01).

Yan Zhang, et al. Blood. 2009 February 12;113(7):1455-1463.
3.
Figure 6

Figure 6. From: Mll5 contributes to hematopoietic stem cell fitness and homeostasis.

Gene expression in Mll5−/− HSC. (A) Genome-wide analysis of gene expression in Mll5−/− and wild-type LSK cells. The plot shows normalized (log2) hybridization signals for individual features on the microarrays probed with Mll5−/− or wild-type labeled cRNA. The data were generated by using 4 independent preparations of RNA from flow-sorted LSK cells (2 preparations from Mll5−/− mice and 2 from littermate controls). In each case, the LSK cells were prepared from bone marrow pooled from 6 mice. (B) Hox gene expression in Mll5−/− LSK cells as determined by the microarray analysis shown in panel A. The graph shows means plus or minus SEM. (C) Quantitative RT-PCR analysis of Hoxb2 and Hoxb5 expression in Mll5−/− LSK cells. Levels of expression were determined in triplicate and normalized to that of β-actin. The graph shows means plus or minus SD for samples from 1 pair of mice. The data are representative of 3 independent experiments.

Yan Zhang, et al. Blood. 2009 February 12;113(7):1455-1463.
4.
Figure 5

Figure 5. From: Mll5 contributes to hematopoietic stem cell fitness and homeostasis.

Cell-cycle status and apoptosis of Mll5−/− HSC. (A, left) Representative FACS data showing Pyronin-Y and DAPI staining of LSK cells from an Mll5−/− mouse and a control littermate. The gates used to discriminate cells in different stages of the cell cycle are shown. (Bottom left quadrant) G0; top left quadrant: G1; top right quadrant: S/G2/M. (Right panel) The graph summarizes cell-cycle data obtained from Mll5−/− and control mice (4 mice per group). (B, left) Representative FACS data showing BrdU incorporation in LSK and myeloid progenitor (Lin, Sca-1, c-kit+) cells from an Mll5−/− mouse and a control littermate following a single injection of BrdU (1 mg) 2 hours before analysis. (Right panel) The graph shows the relative frequencies of BrdU+ cells in LSK and myeloid progenitor populations from Mll5−/− and control mice (4 mice per group). (C, left) Representative FACS data showing annexin-V and DAPI staining of LSK cells in an Mll5−/− mouse and a control littermate. (Right) The graph shows the relative frequencies of apoptotic (annexin-V+ DAPI) LSK cells in Mll5−/− and control littermate mice (7 mice per group).

Yan Zhang, et al. Blood. 2009 February 12;113(7):1455-1463.
5.
Figure 1

Figure 1. From: Mll5 contributes to hematopoietic stem cell fitness and homeostasis.

HSC and progenitor populations in Mll5−/− bone marrow. (A) Representative FACS data showing LT-HSC (Lin, Sca-1+, c-kit+, CD34lo/−, Flt3lo), ST-HSC (Lin, Sca-1+, c-kit+, CD34+, Flt3 lo), MPP (Lin, Sca-1+, c-kit+, CD34+, Flt3+), CMP (Lin, Sca-1, c-kit+, CD34+, FcγRlo), GMP (Lin, Sca-1, c-kit+, CD34+, FcγRhi), MEP (Lin, Sca-1, c-kit+, CD34, FcγRlo), and CLP (Lin, IL-7R+, Sca-1lo, c-kitlo) populations in an Mll5−/− mouse and a wild-type littermate. (B) Graph showing the average relative frequencies of the indicated cell types in wild-type and Mll5−/− mice (n = 7, both groups) determined using the gating strategies shown in panel A. The data were normalized to the wild-type averages and expressed as percentages. Using the Student t test, we discovered that the following populations showed significant differences in frequency between the 2 groups: LT-HSC, ST-HSC, GMP, MEP (all P < .01) and CLP (P < .05). (C) LT-HSC numbers determined in wild-type and Mll5−/− mice (n = 6, both groups) on the basis of CD34 and Flt-3 or CD150 and CD48 expression. The cells for this experiment were obtained from mice that had been backcrossed 9 times to C57BL/6 partners. The differences in LT-HSC numbers were significant in both cases by Student t test (P < .05).

Yan Zhang, et al. Blood. 2009 February 12;113(7):1455-1463.
6.
Figure 3

Figure 3. From: Mll5 contributes to hematopoietic stem cell fitness and homeostasis.

Impaired repopulation of irradiated recipients by Mll5−/− bone marrow cells. (A) Experimental strategy for the competitive repopulation assay. (B) Representative FACS data showing the frequencies of T cells (CD3+), B cells (CD19+), neutrophils (Gr-1hi, CD11b+), monocytes/macrophages (Gr-1+, CD11b+), and LSK cells in recipient mice 3 months after transplantation with mixtures of CD45.2+ test cells (wild-type or Mll5−/−) and CD45.1+CD45.2+ competitor cells. (C) The graph shows the averages of the ratios of test versus competitor populations of the indicated cell types in repopulated mice 3 months after transplantation. The data for each cell type are normalized to the average ratios obtained with mice that received wild-type test cells and are expressed as percentages. A value of less than 100% indicates impaired competitiveness of the test population. The differences between genotypes were statistically significant by Student t test (P < .05 for all comparisons except LSK cells, P < .01). The data are representative of 3 independent experiments performed with bone marrow cells and 1 experiment performed with fetal liver cells (Figure S5). (D) The graph shows the fold decrease in the Mll5−/− repopulation ratios in the 4 independent experiments (relative to wild-type in each experiment).

Yan Zhang, et al. Blood. 2009 February 12;113(7):1455-1463.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk