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Results: 5

1.
Fig. 5.

Fig. 5. From: Mutations alter the sodium versus proton use of a Bacillus clausii flagellar motor and confer dual ion use on Bacillus subtilis motors.

Mutations that affect the ion-coupling capacities of Bs-MotAB and Bs-MotPS. (A) An alignment of a region of Bs-MotB and Bs-MotS (central two lines). The mutational changes introduced into the MotB of Bs-MotAB-LSQ are shown in the top line and those introduced into the MotS of Bs-MotPS are shown in the bottom line. (B) Swimming speed of Bs-MotAB, Bs-MotAB-LSQ, Bs-MotPS, and Bs-MotPS-VAS, as designated by symbols, was assayed as a function of sodium concentration at pH 7.0 (Left) and 9.5 (Right) in TY/20.

Naoya Terahara, et al. Proc Natl Acad Sci U S A. 2008 September 23;105(38):14359-14364.
2.
Fig. 3.

Fig. 3. From: Mutations alter the sodium versus proton use of a Bacillus clausii flagellar motor and confer dual ion use on Bacillus subtilis motors.

Effect of EIPA and CCCP on swimming of B. subtilis expressing different stators. The swimming speed of BS-AB (filled diamond), BS-PS (filled circle), and BCl-AB (filled triangle) in TY medium containing 63 mM sodium was examined at various concentrations of EIPA (A) or CCCP (B). The pH values of the TY medium used for the assays were pH 8.0 (First), 8.5 (Second), 9.0 (Third), and 9.5 (Fourth). The swimming speed was analyzed as described in Materials and Methods.

Naoya Terahara, et al. Proc Natl Acad Sci U S A. 2008 September 23;105(38):14359-14364.
3.
Fig. 2.

Fig. 2. From: Mutations alter the sodium versus proton use of a Bacillus clausii flagellar motor and confer dual ion use on Bacillus subtilis motors.

The motility of a statorless B. subtilis mutant (ΔABΔPS) expressing B. subtilis motAB (BS-AB), B. subtilis motPS (BS-PS), or B. clausii motAB (BCl-AB). (A) Motility in a soft-agar plate assay. Fresh cells were inoculated into LB medium (22) (pH 7.0) soft agar and incubated at 37°C for 16 h. (B–D) Motility in liquid medium. BS-AB (B), BS-PS (C), and BCl-AB (D) cells were grown at 37°C in LB medium (pH 7.0), and the swimming speed was measured at various concentrations of sodium in 1/20 tryptone yeast extract (TY) medium (5) as described in Materials and Methods. The pH values of TY/20 medium used in each assay are designated by symbols as indicated.

Naoya Terahara, et al. Proc Natl Acad Sci U S A. 2008 September 23;105(38):14359-14364.
4.
Fig. 1.

Fig. 1. From: Mutations alter the sodium versus proton use of a Bacillus clausii flagellar motor and confer dual ion use on Bacillus subtilis motors.

Properties of the flagella and swimming of B. clausii KSM-K16. Cells were grown at 30°C in alkaline complex medium (pH 9.0 and 230 mM Na+). (A) Stained flagellae of wild-type B. clausii KSM-K16. Cells were stained with staining solution that contained 5% (wt/vol) tannic acid as described in Materials and Methods. (B and C) The pH values used in the particular assay are designated by symbols that are indicated. (B) The swimming speed of wild-type B. clausii in alkaline complex medium as a function of the sodium concentration and pH. The swimming speed was assayed at various concentrations of sodium as described in Materials and Methods. (C) Effect of EIPA and CCCP on the swimming speed of wild-type B. clausii. Swimming speeds were measured at various concentration of EIPA (Left) or CCCP (Right) in alkaline complex medium containing 230 mM sodium.

Naoya Terahara, et al. Proc Natl Acad Sci U S A. 2008 September 23;105(38):14359-14364.
5.
Fig. 4.

Fig. 4. From: Mutations alter the sodium versus proton use of a Bacillus clausii flagellar motor and confer dual ion use on Bacillus subtilis motors.

Effect of EIPA and CCCP on swimming of B. subtilis expressing wild-type BCI-AB or mutant B. clausii stators. (A) Multiple alignment of the region containing the single transmembrane segment of Oceanobacillus iheyensis MotB (OI_MotB) and MotS (OI_MotS), B. subtilis MotB (BS_MotB), MotS (BS_MotS), and B. clausii MotB (BClMotB). The sequence of B. clausii MotB is highlighted in yellow. The red letters show locations of point mutations in B. clausii MotB mimicking BS_MotB to yield BClMotB-H. The blue letters show locations of point mutations in B. clausii MotB mimicking BS_MotS to yield BClMotB-Na. D26 is critical for flagellar rotation and is highlighted with violet (2). (B) The swimming speeds of strains BCl-AB (filled triangles) and BCl-AB-H (filled circles) were measured in TY/20 medium [pH 9.0 (Left) and pH 9.5 (Right)] at various concentrations of sodium. (C) Effect of EIPA and CCCP on swimming speeds of strains BCl-AB (filled triangles) and BCl-AB-H (filled circles). The swimming speeds were measured in TY medium (pH 9.5, 63 mM Na+) at various concentrations of EIPA (Left) or CCCP (Right). (D) The swimming speeds of strains BCl-AB (filled triangles) and BCl-AB-Na (filled circles) were measured in TY/20 medium [pH 8.5 (Left) and pH 9.0 (Right)] at various concentrations of sodium. (E) Effect of EIPA and CCCP on the swimming speeds of strains BCl-AB (filled triangles) and BCl-AB-Na (filled circles). The swimming speeds were measured in TY medium (pH 8.5, 63 mM Na+) at various concentrations of EIPA (Left) or CCCP (Right).

Naoya Terahara, et al. Proc Natl Acad Sci U S A. 2008 September 23;105(38):14359-14364.

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