We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 5

1.
Figure 3

Figure 3. The Vacuolar H+-ATPase. From: The Yeast Lysosome-like Vacuole: Endpoint and Crossroads.

The vacuolar H+-ATPase (V-ATPase) is a proton pump on the vacuolar membrane composed of a membrane-bound V0 sector and a cytosolic V1 sector. ATP hydrolysis in the V1 sector drives the translocation of protons through the V0 sector.

Sheena Claire Li, et al. Biochim Biophys Acta. ;1793(4):650-663.
2.
Figure 2

Figure 2. Trafficking Pathways to and from the Vacuole. From: The Yeast Lysosome-like Vacuole: Endpoint and Crossroads.

Multiple vesicular pathways deliver proteins to and from the vacuole. Resident vacuolar proteins are sent to the vacuole by several biosynthetic pathways, while proteins targeted for degradaton may be sent via endocytosis or autophagy. Biosynthetic pathways: The ALP pathway delivers cargo from the late Golgi to the vacuole. The CPY pathway also starts from the late Golgi, but traverses multivesicular bodies (MVBs) before reaching the vacuole. The CVT pathway delivers biosynthetic cargo from the cytosol to the vacuole in a process that has common components with autophagy. Trafficking pathways away from the vacuole may recycle membrane and proteins to the endocytic or CPY pathways. Degradative pathways: Endocytosis transports both soluble and membrane-bound cargo from the plasma membrane and extracellular space to the vacuole. Cargo progresses from early endosomes to late endosomes (multivesicular bodies) before reaching the vacuole. In macroautophagy, vesicles called autophagosomes engulf cytosolic material for degradation at the vacuole. In microautophagy, the vacuolar membrane itself forms invaginations which form vesicles that are then degraded at the vacuole.

Sheena Claire Li, et al. Biochim Biophys Acta. ;1793(4):650-663.
3.
Figure 1

Figure 1. Vacuolar morphology is sensitive to growth conditions. From: The Yeast Lysosome-like Vacuole: Endpoint and Crossroads.

The vacuole can change in volume and vesicle number depending on extracellular condtions. Top: Under normal conditions, the cell has two to three medium-sized vacuoles. In hypo-osmotic media, a single large vacuole takes up most of the cell. Upon hyper-osmotic shock, the vacuole fragments into multiple small lobes. Bottom: Superimposed images of fluorescently stained vacuoles on yeast cells viewed under Nomarski optics. Vacuoles were visualized using a C-terminally GFP-tagged V-ATPase subunit (Vma2p-GFP) during log-phase growth under hypotonic conditions (synthetic complete medium), normal conditions (YEPD, buffered to pH 5 (100 mM added salt)), and hypertonic conditions (YEPD, buffered to pH 5 with 1 M NaCl added).

Sheena Claire Li, et al. Biochim Biophys Acta. ;1793(4):650-663.
4.
Figure 5

Figure 5. Stress responses. From: The Yeast Lysosome-like Vacuole: Endpoint and Crossroads.

Panel A: Upon nutrient deprivation, multiple autophagic pathways deliver specific and non-specific cargo to the vacuole. Macroautophagy is the non-specific degradation of cytosolic proteins and organelles. Autophagic membranes originate from pre-autosomal structures (PAS) that enclose cytosolic cargo, forming autophagosomes, which are then delivered to the vacuole. In microautophagy, vacuolar membranes invaginate and directly engulf cargo for degradation. Piecemeal microautophagy of the nucleus (PMN) is a type of autophagy that specifically degrades nuclear compartments, while pexophagy degrades peroxisomes via two mechanisms similar to macroautophagy and microautophagy.
Panel B: Upon salt stress, vacuoles fragment into multiple tiny vesicles. This requires increased production of PI3,5P2 by the Fab1p lipid kinase. Calcium is released from vacuolar stores by Yvc1p, activating calcineurin and the Crz1p transcription factor. This leads to the increased production of the Ena1p Na+/Li+ pump at the plasma membrane and the Pho89p Na+/Pi+ symporter at the vacuole.

Sheena Claire Li, et al. Biochim Biophys Acta. ;1793(4):650-663.
5.
Figure 4

Figure 4. Constitutive Functions of The Yeast Vacuole. From: The Yeast Lysosome-like Vacuole: Endpoint and Crossroads.

The vacuole is responsible for multiple constitutive processes: 1) degradation, 2) storage, 3) buffering, and 4) detoxification. 1) Degradation: The vacuole is highly enriched for hydrolases (yellow circles) that break down cargo delivered via multiple trafficking pathways to the vacuole. 2) Storage: The vacuolar membrane contains multiple transporters (blue polygons) that import amino acids, ions, and metals. Vacuolar acidification by the vacuolar H+ ATPase (V1 and V0, green) is required for proper maturation of hydrolases and for establishing the proton gradient that drives many transporters. 3) Buffering: The vacuolar membrane also contains transporters that recycle amino acids, ions, and metals to the cytosol. The combined activity of importers and exporters is important for ion homeostasis and amino acid recycling. The vacuole is also the main storage site for polyphosphate which buffers cations and is a source for cellular phosphate, 4) Detoxification: The vacuole also sequesters toxic metals and potentially harmful metabolic bi-products via ABC transporters.

Sheena Claire Li, et al. Biochim Biophys Acta. ;1793(4):650-663.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk