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1.
Figure 2

Figure 2. From: Pathogenic Role of NKT and NK Cells in Acetaminophen-Induced Liver Injury is Dependent on the Presence of DMSO.

Effect of DMSO on hepatic IFN-γ mRNA expression following acetaminophen treatment. Following pretreatment with anti-NK1.1 (NKT and NK cell-depleting antibody) or mouse IgG2a (control antibody), mice were treated with saline (controls), APAP (500 mg/kg) in 3.5% DMSO, APAP (500 mg/kg) in saline, or APAP (250 mg/kg) in saline. Relative IFN-γ mRNA levels were measured in the liver at 24 h. Results shown represent mean ± SEM of 8 mice per group. (*) P < 0.05 versus control mice.

Mary Jane Masson, et al. Hepatology. ;48(3):889-897.
2.
Figure 3

Figure 3. From: Pathogenic Role of NKT and NK Cells in Acetaminophen-Induced Liver Injury is Dependent on the Presence of DMSO.

DMSO increases NKT cells in the liver. Total number of hepatic (A) NKT cells (NK1.1+CD3+) and (B) NK cells (NK1.1+CD3-) in the liver 24 h following treatment with DMSO in saline (0%), 3.5%, 5%, or 10%. Data shown are representative of three independent experiments. Values represent the mean ± SEM of 5 mice per group. (*) P < 0.05 versus saline treatment; (#) P < 0.05 versus 5% DMSO treatment.

Mary Jane Masson, et al. Hepatology. ;48(3):889-897.
3.
Figure 6

Figure 6. From: Pathogenic Role of NKT and NK Cells in Acetaminophen-Induced Liver Injury is Dependent on the Presence of DMSO.

DMSO increases NKT cells in the livers of APAP-treated mice. Total number of hepatic (A) NKT (NK1.1+CD3+) and (B) NK (NK1.1+CD3-) cells in the liver 24 h following treatment with APAP (500 mg/kg) in 3.5% DMSO, APAP (500 mg/kg) in saline, or APAP (250 mg/kg) in saline. The total number of hepatic NKT and NK cells in control mice were 1.38 × 105 ± 1.71 × 104 and 1.22 × 105 ± 1.26 × 104, respectively. Data shown are representative of three independent experiments. Values represent the mean ± SEM of 5 mice per group. (*) P < 0.05 versus all other groups.

Mary Jane Masson, et al. Hepatology. ;48(3):889-897.
4.
Figure 1

Figure 1. From: Pathogenic Role of NKT and NK Cells in Acetaminophen-Induced Liver Injury is Dependent on the Presence of DMSO.

Effect of DMSO on the role of NKT and NK cells in acetaminophen-induced liver injury. Following pretreatment with anti-NK1.1 (NKT and NK cell-depleting antibody) or mouse IgG2a (control antibody), male C57Bl/6 mice were treated with (A) APAP (500 mg/kg) in 3.5% DMSO, (B) APAP (500 mg/kg) in saline, or (C) APAP (250 mg/kg) in saline. In (D), female C57Bl/6 mice pretreated with anti-NK1.1 or mouse IgG2a prior to treatment with APAP (500 mg/kg) in saline. Serum ALT activity was measured at 8 h and 24 h as an indicator of liver injury. Results shown represent mean ± SEM of 8-12 mice per group. (*) P < 0.05 versus all other groups.

Mary Jane Masson, et al. Hepatology. ;48(3):889-897.
5.
Figure 4

Figure 4. From: Pathogenic Role of NKT and NK Cells in Acetaminophen-Induced Liver Injury is Dependent on the Presence of DMSO.

DMSO induces IFN-γ and granzyme B expression in hepatic NKT cells. Frequencies of hepatic NKT cells expressing IFN-γ (A and B) and granzyme B (C and D) 24 h following treatment with DMSO (v/v) in saline (0%), 3.5%, 5%, or 10%. The frequencies of IFN-γ+ and granzyme B+ NKT cells were determined by gating on the NKT (NK1.1+CD3+) cell population. Data shown are representative of two independent experiments. Values represent the mean ± SEM of 5 mice per group. (*) P < 0.05 versus saline control. (#) P < 0.05 versus 5% DMSO. Also shown are representative flow cytometry data of IFN-γ (B) and granzyme B (D) expression by NKT cells in mice treated with (0%) or 10%DMSO in saline.

Mary Jane Masson, et al. Hepatology. ;48(3):889-897.
6.
Figure 5

Figure 5. From: Pathogenic Role of NKT and NK Cells in Acetaminophen-Induced Liver Injury is Dependent on the Presence of DMSO.

DMSO induces IFN-γ and granzyme B expression in hepatic NK cells. Frequencies of hepatic NK cells expressing IFN-γ (A and B) and granzyme B (C and D) 24 h following treatment with DMSO in saline (0%), 3.5%, 5%, or 10%. The frequencies of IFN-γ+ and granzyme B+ NK cells were determined by gating on the NK (NK1.1+CD3-) cell population. Data shown are representative of two independent experiments. Values represent the mean ± SEM of 5 mice per group. (*) P < 0.05 versus all other groups. Also shown are representative flow cytometry data of IFN-γ (B) and granzyme B (D) expression by NK cells in mice treated with (0%) or 10% DMSO in saline.

Mary Jane Masson, et al. Hepatology. ;48(3):889-897.
7.
Figure 7

Figure 7. From: Pathogenic Role of NKT and NK Cells in Acetaminophen-Induced Liver Injury is Dependent on the Presence of DMSO.

DMSO induces IFN-γ and granzyme B expression in hepatic NKT cells following APAP treatment. Frequencies of hepatic NKT cells expressing IFN-γ (A and B) and granzyme B (C and D) 24 h following treatment with APAP (500 mg/kg) in 3.5% DMSO, APAP (500 mg/kg) in saline, or APAP (250 mg/kg) in saline. The frequencies of IFN-γ+ and granzymeB+ NKT cells were determined by gating on the NKT (NK1.1+CD3+) cell population. The frequencies of hepatic NKT cells expressing IFN-γ and granzyme B in control mice were 5.72% ± 0.89% and 32.84% ± 2.27%, respectively. Data shown are representative of two independent experiments. Values represent the mean ± SEM of 5 mice per group. (*) P < 0.05 versus all other groups. (#) P < 0.05 versus APAP (500 mg/kg) in saline. Also shown are representative flow cytometry data of IFN-γ (B) and granzymeB (D) expression in NKT cells.

Mary Jane Masson, et al. Hepatology. ;48(3):889-897.
8.
Figure 8

Figure 8. From: Pathogenic Role of NKT and NK Cells in Acetaminophen-Induced Liver Injury is Dependent on the Presence of DMSO.

DMSO induces IFN-γ and granzyme B expression in hepatic NK cells following APAP treatment. Frequencies of hepatic NK cells expressing IFN-γ (A and B) and granzyme B (C and D) 24 h following treatment with APAP (500 mg/kg) in 3.5% DMSO, APAP (500 mg/kg) in saline, or APAP (250 mg/kg) in saline. The frequencies of IFN-γ+ and granzyme B+ NK cells were determined by gating on the NK (NK1.1+CD3-) cell population. The frequencies of hepatic NK cells expressing IFN-γ and granzyme B in control mice were 5.96% ± 1.62% and 5.96% ± 0.88%, respectively. Data shown are representative of two independent experiments. Values represent the mean ± SEM of 5 mice per group. (*) P < 0.05 versus all other groups. (#) P < 0.05 versus APAP (500 mg/kg) in saline. Also shown are representative flow cytometry data of IFN-γ (B) and granzymeB (D) expression in NK cells.

Mary Jane Masson, et al. Hepatology. ;48(3):889-897.

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