Results: 5

1.
Figure 3

Figure 3. Fecundity effects on female flies treated with dsRNA against gmmmgp. From: Analysis of milk gland structure and function in Glossina morsitans: Milk protein production, symbiont populations and fecundity.

Two cages of 30 female flies were treated with either gmmmgp or gfp dsRNAs at 5 and 13 days post eclosion. Numbers of offspring and female mortality were monitored daily. A. Measurement of cumulative pupal deposition per day for each cage. B. Measurement of percentage of surviving females depositing pupa per day

Geoffrey M. Attardo, et al. J Insect Physiol. ;54(8):1236-1242.
2.
Figure 4

Figure 4. In situ staining of Sodalis in pregnant female milk gland. From: Analysis of milk gland structure and function in Glossina morsitans: Milk protein production, symbiont populations and fecundity.

Tissue sections were stained with DAPI and Sodalis specific DIG-labeled 16S ribosomal RNA probe. (A–C: Longitudinal milk gland section). A: DAPI staining of milk gland cell nuclei, B: In situ staining of Sodalis. The staining pattern shows concentrated Sodalis in the extracellular space of the lumen. Bacteria can also be seen intracellularly within the cytoplasm of the secretory cells. C: Merged images: C1- nucleus, C2- cytoplasm (Sodalis), C3- lumen (Sodalis).

Geoffrey M. Attardo, et al. J Insect Physiol. ;54(8):1236-1242.
3.
Figure 5

Figure 5. In situ staining of Wigglesworthia in pregnant female milk gland. From: Analysis of milk gland structure and function in Glossina morsitans: Milk protein production, symbiont populations and fecundity.

Tissue sections were stained with DAPI and Wigglesworthia specific DIG-labeled 16S ribosomal RNA probe. (A–F: Longitudinal milk gland sections). A+D: DAPI staining of milk gland cell nuclei, B+E: In situ staining of Wigglesworthia. Bacteria are concentrated within the lumen and can be seen positioned within the canals leading to the secretory reservoirs. C+F: Merged images: C1- nucleus, C2- secretory reservoir, C3- bacteria within the canal leading to the reservoir (Wigglesworthia), C4- lumen with bacteria (Wigglesworthia), F1- nucleus, F2 – cytoplasm, F3- lumen with concentrated bacteria (Wigglesworthia), F4- lumen.

Geoffrey M. Attardo, et al. J Insect Physiol. ;54(8):1236-1242.
4.
Figure 1

Figure 1. GmmMgp in situ and immunohistochemical staining in pregnant female milk gland. From: Analysis of milk gland structure and function in Glossina morsitans: Milk protein production, symbiont populations and fecundity.

Tissue sections were stained with DAPI, DIG-labeled antisense gmmmgp RNA probe, and Anti-GmmMGP antisera. (A–D: Transverse milk gland section; E–H: Longitudinal milk gland section) - A+E: DAPI staining of milk gland cell nuclei, B+F: In situ staining of gmmmgp transcript. Staining is visible throughout the cytoplasm of the secretory cells with no staining observed in the surrounding fat body. C+G: Immunohistochemical staining of GmmMGP. GmmMGP is concentrated within the extracellular secretory reservoir and the lumen of the milk gland. D+H merged images: D1- nucleus, D2- cytoplasm (gmmmgp transcript), D3- secretory reservoir (GmmMGP), D4- lumen (GmmMGP), H1- nucleus, H2- cytoplasm (gmmmgp transcript), H3- secretory reservoir (GmmMGP), H4- lumen (GmmMGP).

Geoffrey M. Attardo, et al. J Insect Physiol. ;54(8):1236-1242.
5.
Figure 2

Figure 2. GmmTsf in situ and immunohistochemical staining in pregnant female milk gland. From: Analysis of milk gland structure and function in Glossina morsitans: Milk protein production, symbiont populations and fecundity.

Tissue sections were stained with DAPI, DIG-labeled antisense gmmmgp RNA probe, and Anti-GmmMGP antisera. (A–D: Transverse milk gland section; E–H: Longitudinal milk gland section) - A+E: DAPI staining of milk gland cell nuclei. B+F: In situ staining of gmmtsf transcript. Transcript is concentrated within the cytoplasm of the secretory cells with light staining of the surrounding fat body. C+G: Immunohistochemical staining of GmmTsf. GmmTsf staining is specific to the secretory reservoir and the gland lumen. (Reservoirs appear small in this image as this female was approaching the end of the pregnancy cycle. At this point the larva takes milk secretions faster than they can accumulate). D+H: Merged images: D1- nucleus, D2- cytoplasm (gmmtsf transcript), D3- secretory reservoir (GmmTsf), D4- lumen (GmmTsf), H1- nucleus, H2, cytoplasm (gmmtsf transcript), H3- secretory reservoir (GmmTsf), H4- lumen (GmmTsf).

Geoffrey M. Attardo, et al. J Insect Physiol. ;54(8):1236-1242.

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