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Results: 5

1.
Fig. 2

Fig. 2. From: Glucose induction pathway regulates meiosis in Saccharomyces cerevisiae in part by controlling turnover of Ime2p meiotic kinase.

Involvement of Rgt2p and Snf3p in regulating meiosis in colonies grown on glucose medium. Colonies of the wild-type (WT), rgt2Δ, snf3Δ, and rgt2Δsnf3Δ strains used in Fig. 1 were grown for 8 days on SC medium and then transferred to a medium that selects for meiotic cells. Colonies were photographed after 8 days on the latter medium.

Misa Gray, et al. FEMS Yeast Res. ;8(5):676-684.
2.
Fig. 5

Fig. 5. From: Glucose induction pathway regulates meiosis in Saccharomyces cerevisiae in part by controlling turnover of Ime2p meiotic kinase.

Sporulation defect in the rgt1Δ mutant correlates with cell size. (a) Wild-type and rgt1Δ cultures were grown as in Fig. 1 and sizes in the cell population determined. (b) Cultures were grown as in Fig. 1 and cell fractions of the indicated average size (geometric mean) were recovered by centrifugal elutriation, transferred to Sp medium, and assayed for the frequency of asci after 72 h. Wild type (△), rgt1Δ (●).

Misa Gray, et al. FEMS Yeast Res. ;8(5):676-684.
3.
Fig. 3

Fig. 3. From: Glucose induction pathway regulates meiosis in Saccharomyces cerevisiae in part by controlling turnover of Ime2p meiotic kinase.

Role of Rgt2p and Snf3p in regulating IME1 and IME2 transcript levels. Wild-type (WT, SH1232) and rgt2Δsnf3Δ (SH1926) cultures were grown in YPA and transferred to sporulation media as in Fig. 1. At the indicated times after transfer (0, 8, 12, and 24 h), samples were removed from the culture and analyzed for IME1, IME2, and a control (DED1) transcript levels by S1 nuclease sensitivity assays.

Misa Gray, et al. FEMS Yeast Res. ;8(5):676-684.
4.
Fig. 1

Fig. 1. From: Glucose induction pathway regulates meiosis in Saccharomyces cerevisiae in part by controlling turnover of Ime2p meiotic kinase.

Involvement of Rgt2p and Snf3p glucose sensors in repressing sporulation in the presence of glucose. Wild-type (WT, SH1232), rgt2Δ (SH2402), snf3Δ (SH2431), rgt2Δsnf3Δ (SH1926), mth1Δ (SH3993), rgt2Δsnf3Δmth1Δ (SH3961), rgt1Δ (SH3415), and rgt1Δrgt2Δsnf3Δ (SH3362) strains were grown in YPA medium to late-log phase, and then transferred to Sp medium (gray bars), Sp medium+0.5% glucose (black bars), or Sp medium +2% glucose (white bars). After 72 h, these cultures were examined by microscope for the percentage of cells that had formed asci. Error bars represent the SE (n = 3).

Misa Gray, et al. FEMS Yeast Res. ;8(5):676-684.
5.
Fig. 4

Fig. 4. From: Glucose induction pathway regulates meiosis in Saccharomyces cerevisiae in part by controlling turnover of Ime2p meiotic kinase.

Effect of glucose sensors and the Rgt1p transcription factor on Ime2p-6HA stability. Wild-type (WT, SH3354), rgt2Δsnf3Δ (SH3343), and rgt1Δrgt2Δsnf3Δ (SH3342) strains were grown in SC-Leu to midlog, and then transferred to Sp+0.5 (lanes 1–4) or Sp (lanes 5–7). All strains contained pS714 (Purnapatre et al., 2005), a CEN-LEU2 plasmid that carries the tetO-IME2-6XHA allele. Both sporulation media contained tetracycline (2 μg mL−1) to inhibit further transcription from the tetO promoter. Samples were removed at the indicated times after transfer (0, 30, 60, 90 min) and analyzed for Ime2p-6HA and Cdc28p levels by Western blot.

Misa Gray, et al. FEMS Yeast Res. ;8(5):676-684.

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