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Results: 7

1.
Figure 3:

Figure 3:. From: Similar biological characteristics of human embryonic stem cell lines with normal and abnormal karyotypes.

DNA fingerprinting of FY-hES-1 (A), -3 (B), -4 (C), -5 (D), -7 (E), -8 (F) and -3PN (G), respectively.

Xiaofang Sun, et al. Hum Reprod. 2008 October;23(10):2185-2193.
2.
Figure 5:

Figure 5:. From: Similar biological characteristics of human embryonic stem cell lines with normal and abnormal karyotypes.

Histology analysis of teratomas derived from FY-hES-1 and FY-3PN hESC lines.
(A) Squamous epithelium tissue from ectoderm; (B) neural ganglia tissue from ectoderm; (C) cartilage tissue from mesoderm and (D) glandular tissue from endoderm.

Xiaofang Sun, et al. Hum Reprod. 2008 October;23(10):2185-2193.
3.
Figure 7:

Figure 7:. From: Similar biological characteristics of human embryonic stem cell lines with normal and abnormal karyotypes.

X chromosome inactivation analysis of the XX hESC line.
Column A represents inactive X chromosome and column B represents active X chromosome. A normal female blood DNA and FY-hES-1 (46, XY) was used as a control (red peak is the marker and blue peak is the product).

Xiaofang Sun, et al. Hum Reprod. 2008 October;23(10):2185-2193.
4.
Figure 2:

Figure 2:. From: Similar biological characteristics of human embryonic stem cell lines with normal and abnormal karyotypes.

Octamer binding protein 4 expression by RT–PCR.
Lanes 3–9 represent FY-hES-1, -3, -4, -5, -7, -8 and FY-3PN, respectively. Lane 1 is a negative control and lane 2 is human β-actin (265 bp).

Xiaofang Sun, et al. Hum Reprod. 2008 October;23(10):2185-2193.
5.
Figure 4:

Figure 4:. From: Similar biological characteristics of human embryonic stem cell lines with normal and abnormal karyotypes.

Karyotypes of hESC lines.
Karyotype analysis (AG) and FISH images (H) of hESC. Normal 46, XY karyotypes of FY-hES-1 (A), -3 (B) and -4 (C), normal 46, XX of FY-hES-7 (D) and -8 (E). FY-3PN (F) shows a triploid karyotype of 69, XXX. FY-hES-5 (G) shows unbalanced Robertsonian translocations with 46, XX+13, der (13;13) (q10;q10) karyotype. Blue arrow indicates duplicate chromosome 13. FISH of interphase nuclei from FY-hES-5 cells shows two chromosome 13 (red), two chromosome 21 (green), two chromosome 18 (aqua) and two X chromosomes (blue) (H).

Xiaofang Sun, et al. Hum Reprod. 2008 October;23(10):2185-2193.
6.
Figure 6:

Figure 6:. From: Similar biological characteristics of human embryonic stem cell lines with normal and abnormal karyotypes.

RT–PCR analysis of imprinted gene expression (left panel).
Paternally expressed gene IGF2, small nuclear ribonucleoprotein polypeptide N (SNRPN) and maternally expressed gene H19 were analyzed in four hESC lines with different karyotypes. The conditional gene GNAS was used as an additional control. Glyceraldehyde-3-phosphate dehydrogenase (GAPD) was included as mRNA quantitative control. SNRPN gene methylation status (right panel) in FY-hES-1, -5, -7, and -3PN lines, AS, PWS and normal DNA was used as control.

Xiaofang Sun, et al. Hum Reprod. 2008 October;23(10):2185-2193.
7.
Figure 1:

Figure 1:. From: Similar biological characteristics of human embryonic stem cell lines with normal and abnormal karyotypes.

Derivation of hESC lines and characteristics.
(I) Human embryos and isolated ICM. (A) A Day 5 blastocyst cultured in G2.3 blastocyst medium. Note the small ICM. (B) A Day 7 blastocyst that has been cultured in the blastocyst optimum medium for another 2 days. The ICM became clearer. (C) An ICM isolated by mechanical method. (D) A round ICM colony surrounded by a group of residual trophectoderm after mechanical isolation. (E) An ICM isolated by immunosurgery. (F) A dome-like structure on the feeder layer formed after 8 days culture of ICM isolated with immunosurgery. Arrows in C to F indicate ICMs. (G) Typical round hESC colonies with very clear boundary at low magnification. (H) hESC morphology in a colony, showing a high nucleus–cytoplasm ratio; note the presence of nucleoli and typical intercellular spaces at high magnification. Bar = 25 µm in A, B, C and D and bar = 100 µm in E, F and G. (II) Immunocytochemical staining of undifferentiated hESC colonies after 32 passages in four cell lines (A) AP, (B) SSEA-1, (C) SSEA-4, (D) TRA-1-60 and (E) TRA-1-81. Bar = 100 µm.

Xiaofang Sun, et al. Hum Reprod. 2008 October;23(10):2185-2193.

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