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1.
Figure 4

Figure 4. From: Distinct eRF3 Requirements Suggest Alternate eRF1 Conformations Mediate Peptide Release During Eukaryotic Translation Termination.

Eo/Sc eRF1 mutants restore peptide release at the UGA stop codon. A) Endpoint peptide release assay with excess Sc eRF1 and Sc eRF3. B) Kinetics of peptide release with Sc eRF1 (+/− Sc eRF3). C) Kinetics of peptide release with Eo/Sc eRF1 and mutant derivatives (+/− Sc eRF3) at UAA stop codon. D) Kinetics of peptide release with Eo/Sc eRF1 and mutant derivatives (+/− Sc eRF3) at UAG stop codon. E) Kinetics of peptide release with Eo/Sc eRF1 and mutant derivatives (+/− Sc eRF3) at UGA stop codon. Representative data for each experiment is shown. Reactions with eRF1s are denoted by closed symbols, while reactions with eRF1s and Sc eRF3 are denoted by open symbols.

Hua Fan-Minogue, et al. Mol Cell. ;30(5):599-609.
2.
Figure 5

Figure 5. From: Distinct eRF3 Requirements Suggest Alternate eRF1 Conformations Mediate Peptide Release During Eukaryotic Translation Termination.

Effect of reducing eRF3 binding on Eo/Sc E57S/S58N eRF1 function in vivo. A) Readthrough in strains carrying Sc eRF1, Sc CΔ19 eRF1, Eo/Sc E57S/S58N eRF1, or Eo/Sc E57S/S58N/CΔ19 eRF1. B) Western blot quantitation of eRF1 levels in strains above. All strains were grown in SM glucose medium at 30°C. Readthrough values are represented as mean ± SD.

Hua Fan-Minogue, et al. Mol Cell. ;30(5):599-609.
3.
Figure 2

Figure 2. From: Distinct eRF3 Requirements Suggest Alternate eRF1 Conformations Mediate Peptide Release During Eukaryotic Translation Termination.

Efficiency of stop codon recognition mediated by Eo/Sc eRF1 suppressor mutants. (A) Alignment of TASNIKS and YxCxxxF motifs from Saccharomyces eRF1 and Euplotes eRF1. Numbering from Saccharomyces eRF1 is used. (B) Readthrough of stop codons measured in strains expressing wild type Sc eRF1 or Eo/Sc C124S, C124N, A75S or A75S/C124N eRF1. (C) Western blot quantitation of eRF1 levels from strains in (B). (D) Readthrough of stop codons measured in strains expressing wild type Sc eRF1 or Eo/Sc C124S, E57S/S58N, or E57S/S58N/C124S eRF1. (E) Western blot quantitation of eRF1 levels from strains in (D). All strains were grown in SM glucose medium at 35°C. Readthrough values are represented as mean ± SD.

Hua Fan-Minogue, et al. Mol Cell. ;30(5):599-609.
4.
Figure 6

Figure 6. From: Distinct eRF3 Requirements Suggest Alternate eRF1 Conformations Mediate Peptide Release During Eukaryotic Translation Termination.

Models of eukaryotic stop codon recognition and translation termination. A) The location of key determinants of eRF1 function on the three-dimensional structure of human eRF1. Only the relevant portion of domain 1 is shown. Residues identified by genetic screens in this study or by Stansfield and co-workers (Bertram et al., 2000) are indicated in yellow (residues identified in this study are in black type, while those identified by Stansfield and co-workers are in purple type). White circles indicate cavities 1, 2, and 3. The residue designations and numbering corresponds to human eRF1 (+3 relative to Sc eRF1). B) Model for eukaryotic translation termination at UAA/UAG vs. UGA stop codons.

Hua Fan-Minogue, et al. Mol Cell. ;30(5):599-609.
5.
Figure 3

Figure 3. From: Distinct eRF3 Requirements Suggest Alternate eRF1 Conformations Mediate Peptide Release During Eukaryotic Translation Termination.

Efficiency of stop codon recognition mediated by Sc eRF1 carrying C124S or TASNIKS mutations. (A) Readthrough of stop codons measured in strains expressing Sc eRF1 with C124S or viable TASNIKS mutations. (B) Western blot quantitation of eRF1 levels from strains in (A). (C) Readthrough in strains carrying Sc eRF1, depleted of eRF1, or carrying combinations of Sc eRF1 mutations that result in an inability to support growth as the sole source of eRF1. For mutant eRF1 proteins unable to support growth, readthrough was measured using a galactose to glucose shift protocol as described in the Materials and Methods. All strains were grown in SM glucose medium at 35°C. Readthrough values are represented as mean ± SD.

Hua Fan-Minogue, et al. Mol Cell. ;30(5):599-609.
6.
Figure 1

Figure 1. From: Distinct eRF3 Requirements Suggest Alternate eRF1 Conformations Mediate Peptide Release During Eukaryotic Translation Termination.

Mutagenesis of Eo/Sc eRF1. (A) The Eo/Sc eRF1 plasmid used for random mutagenesis and the Sc eRF1 plasmid under GAL1 promoter control used to support growth of the sup45Δ strain while screening for suppressors of the hybrid Eo/Sc eRF1. (B) Growth of strain carrying Eo/Sc C124S eRF1. Plates were incubated at 22°C, 30°C and 35°C for 5 days. (C) Readthrough levels of wild type Sc eRF1, Eo/Sc eRF1 and Eo/Sc C124S eRF1 at 35°C. Readthrough in strains carrying the Eo/Sc eRF1 that cannot support cell growth as the sole source of eRF1 was carried out using a galactose to glucose shift protocol as described in the Materials and Methods. Readthrough values are represented as mean ± SD.

Hua Fan-Minogue, et al. Mol Cell. ;30(5):599-609.

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