Results: 5

1.
Figure 4

Figure 4. From: Notch Activation Is Associated with Tetraploidy and Enhanced Chromosomal Instability in Meningiomas.

Isolated tetraploid cells are viable but have higher spontaneous apoptotic rates compared with diploid cells. (A) Viable diploid G1 (2n) cells were separated from tetraploid G2 (8n) cells using Hoechst Staining and flow cytometry and propagated in culture. (B) After 10 passages in culture, the ploidy of the tetraploid cells was measured by 7-AAD staining for total DNA content and flow cytometry. (C) The percentage of apoptotic cells (y-axis) in SF4068-Vector (Vector) and isolated tetraploid cells from SF4068-HES1 (HES1-T) are shown.

Gilson S Baia, et al. Neoplasia. 2008 June;10(6):604-612.
2.
Figure 2

Figure 2. From: Notch Activation Is Associated with Tetraploidy and Enhanced Chromosomal Instability in Meningiomas.

Tetraploidy in meningioma is associated with features of chromosomal instability and mitotic spindle anomalies. DAPI staining (blue stain) was used to show that SF4068-HES1 cells were associated with an increased frequency of nuclear atypia (A–E) characterized by the presence of micronuclei (arrows), elongated and lobulated nuclei, and nuclear bridges (arrowhead), not found in SF4068-Vector cells (F). Individual nuclei exhibiting atypia were HES1-positive (green stain; compare G–I with J–L). Staining with α-tubulin (red stain, M–O) revealed aberrant mitotic spindles, including multipolar spindles (O) in SF4068-HES1 cells.

Gilson S Baia, et al. Neoplasia. 2008 June;10(6):604-612.
3.
Figure 5

Figure 5. From: Notch Activation Is Associated with Tetraploidy and Enhanced Chromosomal Instability in Meningiomas.

Tetraploid cells develop a higher number of numerical and structural chromosomal abnormalities over time in culture when compared with diploid cells. Spectral karyotyping (SKY) was used to assess numerical and structural abnormalities in SF3061-Vector and isolated diploid and tetraploid cells from SF3061-HES1 stable cell populations. (A) Representative SKY metaphases from control nonneoplastic arachnoid (Arachnoid) cells, SF3061-Vector (Diploid), and SF3061-HES1 (Tetraploid) cells are shown. (B and C) SKY karyograms of a diploid metaphase from SF3061-Vector cells at passage 19 (B) and a tetraploid metaphase from SF3061-HES1 cells at passage 19 (C) are shown. Chromosome numbers (white) are indicated.

Gilson S Baia, et al. Neoplasia. 2008 June;10(6):604-612.
4.
Figure 3

Figure 3. From: Notch Activation Is Associated with Tetraploidy and Enhanced Chromosomal Instability in Meningiomas.

Activated Notch1 and Notch2 induce endogenous HES1 expression and tetraploidy in meningioma cell lines. (A) Western blot analysis of SF4068-Vector (V), SF4068-N1ICD (N1), and SF4068-N2ICD (N2) cells using monoclonal antibodies specific for Notch1 (n1) or Notch2 (n2). α-Tubulin was included as a loading control. (B) Activity of the HES1 promoter was determined using the luciferase reporter assay. Relative luciferase units (y-axis) in SF4068-Vector (V), SF4068-N1ICD (N1), and SF4068-N2ICD (N2) cells are plotted. (C) Immunofluorescence using the HES1 polyclonal antibody (left panels) showed induction and nuclear localization of endogenous HES1 in SF4068-N1ICD (N1) and SF4068-N2ICD (N2) stable cell populations. Nuclei were counterstained with DAPI (right panels). (D) Flow cytometric analysis for total DNA content by 7-AAD was performed in SF4068-Vector (V), SF4068-N1ICD (N1), and SF4068-N2ICD (N2) cells. The number of individual cells (y-axis) is plotted against total DNA content (x-axis). The 2n, 4n, and 8n peaks are indicated above the plots.

Gilson S Baia, et al. Neoplasia. 2008 June;10(6):604-612.
5.
Figure 1

Figure 1. From: Notch Activation Is Associated with Tetraploidy and Enhanced Chromosomal Instability in Meningiomas.

Exogenous expression of HES1 is associated with tetraploidy in meningioma cell lines. HES1 was expressed in meningioma cell lines using retroviral-mediated gene transfer. (A) Western blot analysis using a polyclonal antibody against HES1 in control (lanes 1–3) and stable (lanes 4–6) cell populations. Lane 1, SF4068-Vector; lane 2, SF4433-Vector; lane 3, SF3061-Vector; lane 4, SF4068-HES1; lane 5, SF4433-HES1; lane 6, SF3061-HES1. α-Tubulin was included as a loading control. (B) Immunofluorescence using the HES1 polyclonal antibody (left panel) was used to show nuclear localization of HES1 in SF4068-HES1 stable cell populations. Nuclei were counterstained with DAPI (right panel). Arrow indicates HES1-positive nucleus; arrowhead, HES1-negative nucleus. (C) Immunofluorescent staining for incorporated BrdU and total DNA content by 7-AAD, followed by biparametric BrdU/7-AAD flow cytometric analysis was performed in Vector and HES1 stable cell populations. The BrdU fluorescence (y-axis) for SF4068-Vector and SF4068-HES1 cells is plotted against total DNA content (x-axis). (D) The number of individual cells (y-axis) for the indicated Vector or HES1 stable cell populations is plotted against total DNA content (x-axis). The 2n, 4n, and 8n peaks are indicated above the plots.

Gilson S Baia, et al. Neoplasia. 2008 June;10(6):604-612.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk