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Results: 4

1.
Figure 1

Figure 1. From: Biological and Technical Variables Affecting Immunoassay Recovery of Cytokines from Human Serum and Simulated Vaginal Fluid: A Multicenter Study.

Assay accuracy for predicted measurements of 25 IU spikes of IL-1β 86/552 (A), IL-1β 86/680 (B), and IL-6 89/548 (C) across laboratories and kits. Values were derived from a linear regression curve with the standard error of the mean slope represented by error bars. The horizontal dashed line indicates nominal weight/volume concentrations of 25 IU/mL, which is 228.67 pg/mL for IL-1β 86/552 and 250 pg/mL for IL-1β 86/680 and IL-6 89/548.

Raina N. Fichorova, et al. Anal Chem. 2008 June 15;80(12):4741-4751.
2.
Figure 2

Figure 2. From: Biological and Technical Variables Affecting Immunoassay Recovery of Cytokines from Human Serum and Simulated Vaginal Fluid: A Multicenter Study.

Interlaboratory (A) and interassay (B) comparison of results obtained for IL-1β 86/552, IL-1β 86/680, and IL-6 89/548 cytokines from four laboratories using the same Quantikine ELISA (A) and from laboratory A using four assay types (B). Each line represents linear regression analysis between nominal values (WHO IU/mL) of the low, middle, and high spike of each NIBSC standard and their measured concentrations (pg/mL) in one assay run.

Raina N. Fichorova, et al. Anal Chem. 2008 June 15;80(12):4741-4751.
3.
Figure 4

Figure 4. From: Biological and Technical Variables Affecting Immunoassay Recovery of Cytokines from Human Serum and Simulated Vaginal Fluid: A Multicenter Study.

Effect of matrix on percent recovery of the middle spike of the IL-6 NIBSC cytokine (A) and assay-specific calibrators (B). Box plots represent medians and interquartile range of each assay. The horizontal solid line represents the median from all assays. The P values indicate probability of matrix effects for each assay type where n.s. is a nonsignificant (P > 0.01) effect of matrices (Kruskal−Wallis ANOVA, Dunn’s Post Hoc tests).

Raina N. Fichorova, et al. Anal Chem. 2008 June 15;80(12):4741-4751.
4.
Figure 3

Figure 3. From: Biological and Technical Variables Affecting Immunoassay Recovery of Cytokines from Human Serum and Simulated Vaginal Fluid: A Multicenter Study.

Effect of matrix on percent recovery of the middle spike of IL-1β NIBSC cytokines (A and B) and assay-specific calibrators (C). Box plots represent medians and interquartile range of each assay. The horizontal solid line represents the median from all assays. The P values indicate probability of matrix effects for each assay type where n.s. is a nonsignificant (P > 0.01) effect of matrices (Kruskal−Wallis ANOVA, Dunn’s Post Hoc tests).

Raina N. Fichorova, et al. Anal Chem. 2008 June 15;80(12):4741-4751.

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