We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 4

1.
Figure 4

Figure 4. Efficacy of OPAL immunotherapy.. From: Control of Viremia and Prevention of AIDS following Immunotherapy of SIV-Infected Macaques with Peptide-Pulsed Blood.

Antiretroviral therapy (ART) was withdrawn at week 10, after the last vaccination, and (A) plasma SIV RNA followed. The 26 animals that controlled viremia on ART are illustrated with mean±standard error of vaccine groups. (B) Survival of these 26 vaccinated and controls animals is shown. P values represent the difference between controls and the combined vaccine groups (see Table 1).

Robert De Rose, et al. PLoS Pathog. 2008 May;4(5):e1000055.
2.
Figure 3

Figure 3. SIV-specific antibody responses.. From: Control of Viremia and Prevention of AIDS following Immunotherapy of SIV-Infected Macaques with Peptide-Pulsed Blood.

All 32 live macaques had serial measurements of SIV-specific antibodies utilizing HIV-2 Western Blot strips. (A) Representative examples of the evolution of the Western Blot profiles of a macaque within each vaccine group. A positive control sample from HIV-2 infected (+) and uninfected (−) human subjects are shown at the right of the panel. (B) Mean±standard error densitometry measurements of Gag (anti-p26) responses and (C) Env (anti-gp36) responses over time.

Robert De Rose, et al. PLoS Pathog. 2008 May;4(5):e1000055.
3.
Figure 2

Figure 2. Non-Gag T cell immunogenicity of OPAL Vaccination.. From: Control of Viremia and Prevention of AIDS following Immunotherapy of SIV-Infected Macaques with Peptide-Pulsed Blood.

SIV-specific CD4 and CD8 T-cells expressing IFNγ were studied over time by intracellular cytokine staining to Env (A, B), Pol (C, D) and a pool of overlapping peptides spanning combined Regulatory/Accessory proteins (RTNVVV, E, F). Mean±standard error of vaccine groups compared to control unvaccinated animals (circles) is shown. Four initial vaccinations were given weeks 4–10 and a second set of 3 immunizations given weeks 36–42 as shown in Fig 1A.

Robert De Rose, et al. PLoS Pathog. 2008 May;4(5):e1000055.
4.
Figure 1

Figure 1. T-cell immunogenicity of OPAL vaccination.. From: Control of Viremia and Prevention of AIDS following Immunotherapy of SIV-Infected Macaques with Peptide-Pulsed Blood.

SIV Gag-specific CD4 (A) and CD8 (B) T-cells expressing IFNγ were studied over time by intracellular cytokine staining. Mean±standard error of vaccine groups compared to control unvaccinated animals (circles) is shown. The primary OPAL vaccinations of macaques (arrows, weeks 4, 6, 8 and 10 after SIVmac251 infection) consisted of autologous PBMC pulsed with either overlapping SIV Gag 15mer peptides (OPAL-Gag, triangles) or peptides spanning all 9 SIV proteins (OPAL-All, squares). Initial vaccinations were given under the cover of antiretroviral treatment (ART). Animals were re-boosted with OPAL immunotherapy in the same randomised groups, without ART, at weeks 39, 42 and 42. At week 12, two weeks after the last vaccination, CD4 (C) and CD8 (D) T-cell responses to pools of overlapping peptides spanning SIV Gag, Env, Pol or combined Regulatory/Accessory proteins (Nef, Tat, Rev, Vif, Vpx, Vpr [Reg]) were assessed in all animals by intracellular cytokine staining. In addition, responses to a SIV Gag CD8 T-cell epitope KP9, were assessed by a Mane-A*10/KP9 tetramer. Mean±standard error of vaccine groups is shown along with 2-sided t-test p values of <0.10. (E) SIV Gag specific CD8 T-cell responses correlated inversely with CD8 T-cell responses to the summation of non-Gag (Env+Pol+Regulatory protein) responses across all 21 live OPAL-immunized animals. The animals with >50% CD8 T-cell responses to the combined pool had total responses of 50.4% and 54.5%, primarily to Env (50.1% and 54.2% respectively). Spearman rank correlation is shown.

Robert De Rose, et al. PLoS Pathog. 2008 May;4(5):e1000055.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk