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Results: 4

1.
Fig. 4

Fig. 4. From: Strategies and challenges in eliciting immunity to melanoma.

Restricted trafficking of BMDCs and activation of CD81 T cells in the axillary/brachial LNs. C57BL/6 mice were immunized subcutaneously with 105 SIINFEKL-pulsed BMDCs four days prior to adoptive transfer of 106 CFSE-labeled OT-I cells. Mice were treated with FTY720 where indicated. The indicated lymphoid organs were harvested, and OT-I cell proliferation was analyzed by CFSE dilution.

Andrew R. Ferguson, et al. Immunol Rev. ;222:28-42.
2.
Fig. 1

Fig. 1. From: Strategies and challenges in eliciting immunity to melanoma.

Endogenous Tyr369 is presented by a radioresistant cell, while tumor-derived Tyr369 is both cross-presented and directly presented by the tumor. In peripheral LN draining normal, on-inflamed tissue, tyrosinase is not presented by Langerhans cells or DCs draining from the skin (top, blue box). Rather, tyrosinase is expressed within the LN, and is likely directly presented to tyrosinase-specific T cells inducing activation and subsequent deletion of self-reactive cells. In contrast, in late stage tumor-bearing mice (bottom, red box), antigen is both cross-presented by dendritic cells, as well as directly presented in the LN by metastatic tumor. In this context, T cells are activated, and though they are not deleted, these cells have impaired effector function.

Andrew R. Ferguson, et al. Immunol Rev. ;222:28-42.
3.
Fig 2

Fig 2. From: Strategies and challenges in eliciting immunity to melanoma.

Comparison of functional avidities of primary and recall effector CD8+ T cells for Tyr369 and Tyr369(Y) in tyrosinaseneg and tyrosinase+ AAD+ mice. CD8+ T cells from primary (Prim) or recall (Rec) responses following immunization of albino (Alb) or tyrosinase+ (Tyr+) mice with Tyr369(Y) were incubated with C1R-AAD stimulator cells pulsed with increasing concentrations of Tyr369 (open symbols) or Tyr369(Y) (closed symbols) and assessed for activation by costaining for CD8 and the intracellular accumulation of IFNγ. Data plotted are the mean of 2 or 3 mice per group +/- standard error of mean. Data has been normalized to the maximum response obtained by each T cell population after stimulation with the peptide used to generate the T cell response. Data are from one of four similar experiments.

Andrew R. Ferguson, et al. Immunol Rev. ;222:28-42.
4.
Fig. 3

Fig. 3. From: Strategies and challenges in eliciting immunity to melanoma.

Administration of IL-12, but not disruption of PD-1-B7-H1 interaction, induces CD81 T-cell differentiation in tumor-draining LNs of late stage tumor-bearing mice. C57BL/6 mice were injected with 4 × 105 B16-OVA at the indicated day before adoptive transfer of 2 × 106 carboxyfluoresein succinimidyl ester (CFSE)-labeled OT-I cells. (A) Mice were injected intraperitoneally with 100 μg of the blocking antibody to B7-H1 (10B5, provided by Dr. Leiping Chen) or control hamster IgG every other day starting 1 day before adoptive transfer. Five days after adoptive transfer, tumor-draining LNs were harvested, and OT-I cells were assessed for IFNγ production. (B) Mice were either untreated or treated with 1 μg IL-12 at the time of adoptive transfer. Five days later, the mediastinal LN was harvested and OT-I cells were assessed for the production of IFNγ.

Andrew R. Ferguson, et al. Immunol Rev. ;222:28-42.

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