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1.
FIG. 1.

FIG. 1. From: Modulation of the Bovine Trophoblastic Innate Immune Response by Brucella abortus .

Infection of cultured bovine CAM explants with Brucella abortus. (A) CAM explants in a six-well plate with the trophoblastic side toward the well in the center of the ring. (B) Immunohistochemical localization of B. abortus (arrows) within trophoblastic cells in a cultured CAM explant. (C and D) CAM cultured for 48 h. Trophoblastic cells are indicated by arrows, and the amnion is indicated by the arrowhead. H&E staining.

Alcina V. Carvalho Neta, et al. Infect Immun. 2008 May;76(5):1897-1907.
2.
FIG. 4.

FIG. 4. From: Modulation of the Bovine Trophoblastic Innate Immune Response by Brucella abortus .

Expression of proinflammatory chemokines and cytokines by CAM explants infected with Brucella abortus at 6 (white bars) and 12 (gray bars) h after inoculation. Normalized CT values for CXCL6 (GCP-2) and CXCL8 (IL-8) are significantly different between 6 and 12 h after inoculation (*, P = 0.0172; **, P = 0.0184). Data points represent geometric means and standard errors for five independent experiments.

Alcina V. Carvalho Neta, et al. Infect Immun. 2008 May;76(5):1897-1907.
3.
FIG. 2.

FIG. 2. From: Modulation of the Bovine Trophoblastic Innate Immune Response by Brucella abortus .

Time course of invasion of bovine trophoblastic cells in CAM explants by Brucella abortus. Explants were inoculated with an MOI of 1,000 of B. abortus strain 2308, treated with gentamicin to kill extracellular bacteria, lysed, serially diluted, and plated for CFU counting. Data points represent means and standard errors of the mean for five independent experiments performed in triplicate.

Alcina V. Carvalho Neta, et al. Infect Immun. 2008 May;76(5):1897-1907.
4.
FIG. 3.

FIG. 3. From: Modulation of the Bovine Trophoblastic Innate Immune Response by Brucella abortus .

Validation of the microarray results by real-time RT-PCR, indicating downregulated expression of proinflammatory genes in CAM explants infected with Brucella abortus in comparison to the uninfected group at 4 h postinoculation. Bars represent geometric means and standard errors of the mean for three pools of two placentas each, totaling six independent experiments. Abbreviations: C9, complement component 9; LFT╬▓, lymphotoxin beta; SAA4, serum amyloid A4; TNF M8, TNF (ligand) superfamily member 8.

Alcina V. Carvalho Neta, et al. Infect Immun. 2008 May;76(5):1897-1907.
5.
FIG. 6.

FIG. 6. From: Modulation of the Bovine Trophoblastic Innate Immune Response by Brucella abortus .

Bovine placentomes from cows experimentally infected with Brucella abortus. (A) Histologically normal placentome from a bacteriologically negative cow. Chorionic villi within caruncular crypts are indicated by the arrow; H&E staining. (B) Same tissue sample as shown in panel A, with a negative result on immunohistochemistry; streptavidin-peroxidase staining. (C) Suppurative placentitis with a moderated and multifocal inflammatory infiltrate (arrow) in a cow with positive bacteriology and normal parturition. (D) Same tissue sample as shown in panel C, with immunohistochemical labeling of cell-associated B. abortus antigens; streptavidin-peroxidase staining. (E) Severe necrotizing placentitis with intense and multifocal inflammatory infiltrate associated with a myriad of intralesional bacterial colonies (arrow) in a cow with positive bacteriology and abortion; H&E staining. (F) Same tissue sample as shown in panel E, with a large colony of immunohistochemically labeled B. abortus; streptavidin-peroxidase staining.

Alcina V. Carvalho Neta, et al. Infect Immun. 2008 May;76(5):1897-1907.
6.
FIG. 5.

FIG. 5. From: Modulation of the Bovine Trophoblastic Innate Immune Response by Brucella abortus .

Expression of proinflammatory chemokines and cytokines by placental tissue of cows experimentally infected with Brucella abortus divided in two groups: (i) positive bacteriology and term parturition (gray bars) and (ii) positive bacteriology with abortion (white bars). Severalfold change of expression was calculated in comparison to the control group level (bacteriologically negative and normal parturition). *, Normalized CT values for CXCL6 (GCP-2) and CXCL8 (IL-8) are significantly different between controls and both positive bacteriology groups combined (P < 0.05; means were compared by Student Newman Keuls test after logarithmic transformation of the raw data). Columns represent geometric means and standard errors.

Alcina V. Carvalho Neta, et al. Infect Immun. 2008 May;76(5):1897-1907.

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