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Results: 5

1.
Fig. 4.

Fig. 4. From: Mesenchymal-epithelial interactions involving epiregulin in tuberous sclerosis complex hamartomas.

Effect of epiregulin on proliferation of human epidermal keratinocytes. Cells were grown and treated with human recombinant epiregulin as described in Materials and Methods. Cell proliferation was quantified by the amount of BrdU incorporation using chemiluminescence ELISA. Data are means ± SD of triplicate experiments. *, P < 0.01 versus control.

Shaowei Li, et al. Proc Natl Acad Sci U S A. 2008 March 4;105(9):3539-3544.
2.
Fig. 2.

Fig. 2. From: Mesenchymal-epithelial interactions involving epiregulin in tuberous sclerosis complex hamartomas.

Immunoreactivity for phosphorylated ribosomal protein S6 in the epidermis and dermis of TSC skin tumors but not normal-appearing skin. Sections of normal-appearing skin (A and B) or periungual fibromas (C and D) were incubated with rabbit anti-phospho-S6 (1:200) (A and C) or control rabbit IgG) (B and D) and stained with Vectastain ABC-alkaline phosphatase with Vector red substrate). Patterns of staining were similar in three periungual fibromas and four angiofibromas from four patients.

Shaowei Li, et al. Proc Natl Acad Sci U S A. 2008 March 4;105(9):3539-3544.
3.
Fig. 1.

Fig. 1. From: Mesenchymal-epithelial interactions involving epiregulin in tuberous sclerosis complex hamartomas.

The epidermis of TSC skin tumors shows increased staining for Ki-67 compared with normal-appearing skin. Sections of patient's normal skin (A and D), angiofibromas (B and C), and periungual fibromas (E and F) from two TSC patients were stained with anti-Ki-67 antibody, with normal mouse IgG1 (C and F) as negative controls for A and B, and D and E, respectively. (Scale bars, 35 μm.) Results were similar with three angiofibromas and five periungual fibromas from five patients.

Shaowei Li, et al. Proc Natl Acad Sci U S A. 2008 March 4;105(9):3539-3544.
4.
Fig. 3.

Fig. 3. From: Mesenchymal-epithelial interactions involving epiregulin in tuberous sclerosis complex hamartomas.

Immunoprecipitation of epiregulin from medium of TSC skin tumor cells. PF cells or fibroblasts from normal-appearing skin (NL) from the same TSC patient were grown in 10% FBS/DMEM without or with 100 ng/ml EGF and labeled with [35S]methionine for 24 h. Proteins precipitated from conditioned medium with anti-epiregulin antibody or normal rabbit IgG were separated by SDS/PAGE to identify a band corresponding to epiregulin secreted by TSC skin tumor cells. Added recombinant epiregulin competed with radiolabeled endogenous epiregulin; absence of the band indicates antibody specificity.

Shaowei Li, et al. Proc Natl Acad Sci U S A. 2008 March 4;105(9):3539-3544.
5.
Fig. 5.

Fig. 5. From: Mesenchymal-epithelial interactions involving epiregulin in tuberous sclerosis complex hamartomas.

Effect of epiregulin on phosphorylation of S6 ribosomal protein in keratinocytes. After incubation for 48 h in keratinocyte-SFM (without added EGF and bovine pituitary extract), cells were incubated without or with 100 ng/ml epiregulin for the indicated times (A and C) or without or with epiregulin at the indicated concentrations for 1 h (B and D) before separation of cell proteins by SDS/PAGE and reaction of Western blots with antibodies against S6 and phosphorylated S6. Shown are representative blots (A and B) and corresponding graphs from triplicate experiments (C and D), in which values are expressed as the means ± SD of the ratios of phospho- to total S6 for treated (at each time or concentration) relative to that of control (time = 0 or concentration = 0). *, P < 0.05; **, P < 0.01 vs. control.

Shaowei Li, et al. Proc Natl Acad Sci U S A. 2008 March 4;105(9):3539-3544.

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