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Results: 5

1.
Fig. 1

Fig. 1. From: An Immunoglobulin Binding Protein (Antigen 5) of the Stable Fly (Diptera: Muscidae) Salivary Gland Stimulates Bovine Immune Responses.

SDS-PAGE analysis of rAg5 stained with Coomassie blue (A) and SGE stained with silver stain (B) depicting the dominant 27 KD protein (native Ag5) of the extract.

M. AMERI, et al. J Med Entomol. ;45(1):94-101.
2.
Fig. 3

Fig. 3. From: An Immunoglobulin Binding Protein (Antigen 5) of the Stable Fly (Diptera: Muscidae) Salivary Gland Stimulates Bovine Immune Responses.

Detection of rising antibody concentration against rAg5 in immunized calves following 21, 28, and 42 days post immunization. Serum samples were diluted 1:1000 and reacted with rAg5 (1 μg/mL) on the solid phase of ELISA plates. Each point represents the average value of the OD450 obtained from duplicate ELISA results.

M. AMERI, et al. J Med Entomol. ;45(1):94-101.
3.
Fig. 2

Fig. 2. From: An Immunoglobulin Binding Protein (Antigen 5) of the Stable Fly (Diptera: Muscidae) Salivary Gland Stimulates Bovine Immune Responses.

Lymphocyte proliferation assay determined by incorporation of tritiated thymidine defined as counts per minute of radioactivity. Lymphocytes from four steers were treated with five separate conditions: no stimulation (no mitogen), stimulated with Con A alone, Con A plus 3.0 μg/mL of rAg5, control protein (CP) or crude SGE. Error bars represent one standard error of the mean of three replicate experiments.

M. AMERI, et al. J Med Entomol. ;45(1):94-101.
4.
Fig. 5

Fig. 5. From: An Immunoglobulin Binding Protein (Antigen 5) of the Stable Fly (Diptera: Muscidae) Salivary Gland Stimulates Bovine Immune Responses.

Western blot immunoassay demonstrates immunoglobulin-binding properties of the natural Ag5 from SGE (A) and rAg5 (B). Lanes 1 and 2 were incubated with serum from two different steers that had been exposed to stable fly bites; lanes 3 and 4 were incubated with serum from two newborn calves collected prior to ingestion of colostrum. Lanes 1−5 were incubated with HRP-sheep-anti-bovine IgG (H + L). Lanes 6−8 were incubated with HRP-bovine IgG Fab, HRP-bovine IgG Fc, or HRP-bovine IgG F(ab’)2, respectively. Molecular weight markers (Rainbow markers; Amersham, Piscataway, NJ) included 35, 30, and 20 kDa.

M. AMERI, et al. J Med Entomol. ;45(1):94-101.
5.
Fig. 4

Fig. 4. From: An Immunoglobulin Binding Protein (Antigen 5) of the Stable Fly (Diptera: Muscidae) Salivary Gland Stimulates Bovine Immune Responses.

Lymphocyte proliferation assay determined by incorporation of tritiated thymidine defined as counts per minute of radioactivity. Lymphocytes collected from eight calves at 21 and 28 DPI were not stimulated (medium alone) or stimulated with rAg5 (3.0 μg/mL). Three calves (Nos. 22, 23, 73) were immunized with rAg5, and control calf 18 received adjuvant with control protein (A), whereas three calves (Nos. 19, 20, 21) were immunized with natural Ag5 isolated from a preparative acrylamide gel, and control calf 19 received adjuvant plus preparative gel lacking protein. Error bars represent one standard error of the mean (n = 3).

M. AMERI, et al. J Med Entomol. ;45(1):94-101.

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