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Results: 3

1.
FIG. 1.

FIG. 1. From: Different Pathways to Acquiring Resistance Genes Illustrated by the Recent Evolution of IncW Plasmids .

Gene load in the IncW backbone. The insertions of the different MGEs in the IncW backbone that originated the five plasmid isolates are represented. Coding sequences (CDSs) and other sequence features belonging to the IncW backbone are gray, white, or gray-white combinations, depending on their assignment to functional modules. Plasmid-specific genes are represented by the color scheme indicated in the row of rectangles (at right) with inscribed plasmid names. The 5′ and 3′ conserved sequences of the integron platform are brown. When the 5′ or 3′ portion of a given CDS has been deleted, it is indicated by a prime (′) before or after the CDS name, respectively. The deletion sites within transposons are marked by vertical lines.

Carlos Revilla, et al. Antimicrob Agents Chemother. 2008 April;52(4):1472-1480.
2.
FIG. 2.

FIG. 2. From: Different Pathways to Acquiring Resistance Genes Illustrated by the Recent Evolution of IncW Plasmids .

Alignments of the TrwA and oriT sequences. Alignments of the TrwA (panel A) and oriT (panel B) sequences of R388/pIE522/pSa, pIE321, and R7K are shown. Invariant nucleotides or amino acids in all plasmids are shadowed in dark gray, while those identical in most plasmids are shadowed in light gray. (A) Residues 8, 10, and 12, which are important for oriT recognition by TrwAR388, are indicated by vertical arrows. (B) The oriT DNA strand complementary to that cleaved by the relaxase is shown. The nic site is represented by a black triangle. Structural motif IR2 is represented by convergent horizontal arrows. The sites of interaction of oriTR388 with proteins IHF (ihfA and ihfB) and TrwA (sbaA and sbaB) are marked by horizontal lines. The start codon of gene trwA (trwA start), its putative ribosome binding site (rbs), and the trwA promoter −10 and −35 sequences are indicated.

Carlos Revilla, et al. Antimicrob Agents Chemother. 2008 April;52(4):1472-1480.
3.
FIG. 3.

FIG. 3. From: Different Pathways to Acquiring Resistance Genes Illustrated by the Recent Evolution of IncW Plasmids .

Junction sequences of specific insertions. Each insertion is represented at the right by a diagram following the color scheme used in Fig. 1 and is preceded by two DNA sequences. The top one represents the backbone sequence (gray) where the insertion takes place. The exact point of insertion is depicted by a vertical arrow. The bottom sequence contains the corresponding insertion colored according to the same scheme. Target duplications of the backbone sequence are shown in bold italics. Individual base changes with respect to the backbone sequence are underlined. In panel C, two green triangles indicate suggested recombination crossover points flanking the aadA13 integrated cassette. Gene aadA13 start and stop codons as well as the complementary sequence of the nuc stop codon are boxed. A putative ribosome binding site is shown in bold. Core (R′), inverse core (R"), and internal IR (L′ and L") sequences of the potentially recircularized aadA13 cassette are signaled by horizontal green arrows, while the composite core site of the integrated cassette is indicated by a horizontal gray arrow. ′osa, 5′ portion of osa has been deleted; ′nuc, 5′ portion of nuc has been deleted.

Carlos Revilla, et al. Antimicrob Agents Chemother. 2008 April;52(4):1472-1480.

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