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Results: 4

1.
FIG. 4.

FIG. 4. From: The Periplasmic Disulfide Oxidoreductase DsbA Contributes to Haemophilus influenzae Pathogenesis .

Effect of dsbA mutation on the virulence of H. influenzae type b in infant rats. Strains were inoculated i.p. into 5-day-old infant rats. The symbols indicate data for individual animals, and the dashed lines indicate the averages. An asterisks indicates that the P value is <0.001. The lower limit of detection (LLD) was 500 CFU/ml. Values less than the lower limit of detection indicate that bacteremia was not detected.

Charles V. Rosadini, et al. Infect Immun. 2008 April;76(4):1498-1508.
2.
FIG. 3.

FIG. 3. From: The Periplasmic Disulfide Oxidoreductase DsbA Contributes to Haemophilus influenzae Pathogenesis .

Effect of hbpA mutation on survival of H. influenzae in the mouse model of bacteremia. Strains were inoculated i.p. into mice, and bacteremia was assessed after 24 h. The symbols indicate data for individual animals, and the dashed lines indicate the averages. The lower limit of detection (LLD) was 500 CFU/ml. Values less than the lower limit of detection indicate that bacteremia was not detected. (A) Single-strain infection. (B) Coinfections with the experimental strains indicated and reference strain RdlacZ. The competitive index is the ratio of the LacZ experimental strain to LacZ+ reference strain RdlacZ. The asterisk indicates that the P value is <0.001.

Charles V. Rosadini, et al. Infect Immun. 2008 April;76(4):1498-1508.
3.
FIG. 1.

FIG. 1. From: The Periplasmic Disulfide Oxidoreductase DsbA Contributes to Haemophilus influenzae Pathogenesis .

Effect of dsbA mutation on survival of H. influenzae in the mouse model of bacteremia. Strains were inoculated i.p. into mice, and bacteremia was assessed after 24 h. The symbols indicate data for individual animals, and the dashed lines indicate the averages. The lower limit of detection (LLD) was 500 CFU/ml. Values less than the lower limit of detection indicate that bacteremia was not detected. (A) Single-strain infection. The asterisk indicates that the P value is 0.039 (t test). (B) Coinfections with the experimental strains indicated and reference strain RdlacZ. The competitive index is the ratio of the LacZ experimental strain to LacZ+ reference strain RdlacZ. The asterisk indicates that the P value is <0.001 (ANOVA with Bonferroni's multiple-comparison test).

Charles V. Rosadini, et al. Infect Immun. 2008 April;76(4):1498-1508.
4.
FIG. 2.

FIG. 2. From: The Periplasmic Disulfide Oxidoreductase DsbA Contributes to Haemophilus influenzae Pathogenesis .

Effects of dsbA mutation on HbpA protein levels and thiol redox state. (A) Detection of HbpA levels in DsbA+ and DsbA strains. Whole-cell lysates of duplicate cultures of RhbpAC (complemented hbpA deletion mutant carrying hbpA-HA in the xyl locus) (lanes 1 and 2) and RhbpACΔdsbA (RhbpAC with dsbA deletion) (lanes 3 and 4) were resolved by 8% SDS-PAGE under reducing conditions and detected by anti-HA Western blotting (left panel). Equal sample concentrations were verified by Coomassie blue staining (right panel). (B) Differential modification of thiols on HbpA in the dsbA mutant compared to the wild type. Spheroplasts were prepared from log-phase cultures, resolved by 8% SDS-PAGE under nonreducing conditions, and detected by anti-HA Western blotting. The strains were the same as those used for panel A and were treated (+) or not treated (−) with 5 mM MPB as indicated. The arrows indicate the oxidized form of HbpA (ox), the reduced form of HbpA (red), and the reduced form of HbpA with thiols modified with MPB (red + MPB).

Charles V. Rosadini, et al. Infect Immun. 2008 April;76(4):1498-1508.

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