Results: 5

1.
FIG. 4.

FIG. 4. From: Proteomic, Microarray, and Signature-Tagged Mutagenesis Analyses of Anaerobic Pseudomonas aeruginosa at pH 6.5, Likely Representing Chronic, Late-Stage Cystic Fibrosis Airway Conditions .

MS/MS spectrum of the differentially expressed peptide VVQPEYNK at m/z 488.77. Predicted masses for the ions of type b and y are shown above and below the sequence, respectively. Ions observed in the spectrum are underlined.

Mark D. Platt, et al. J Bacteriol. 2008 April;190(8):2739-2758.
2.
FIG. 2.

FIG. 2. From: Proteomic, Microarray, and Signature-Tagged Mutagenesis Analyses of Anaerobic Pseudomonas aeruginosa at pH 6.5, Likely Representing Chronic, Late-Stage Cystic Fibrosis Airway Conditions .

Chromatograms from FT-ICR analysis of anaerobic P. aeruginosa digests. Total ion chromatograms 1 (A) and 2 (B) from anaerobic FT-ICR analysis are shown. Note the complexity as well as the reproducibility of the chromatography samples between replicate analyses.

Mark D. Platt, et al. J Bacteriol. 2008 April;190(8):2739-2758.
3.
FIG. 1.

FIG. 1. From: Proteomic, Microarray, and Signature-Tagged Mutagenesis Analyses of Anaerobic Pseudomonas aeruginosa at pH 6.5, Likely Representing Chronic, Late-Stage Cystic Fibrosis Airway Conditions .

2-D gels of aerobically (A and C) and anaerobically (B and D) expressed P. aeruginosa proteins. Cell extracts (30 μg) derived from the same growth phase (see Fig. S1 in the supplemental material) grown with NO3 (A and B)- or NO2 (C and D)-containing medium were separated by 2-D gel electrophoresis and stained with silver nitrate. The numbered spots correspond to proteins identified by MALDI-TOF analysis (Table 1).

Mark D. Platt, et al. J Bacteriol. 2008 April;190(8):2739-2758.
4.
FIG. 3.

FIG. 3. From: Proteomic, Microarray, and Signature-Tagged Mutagenesis Analyses of Anaerobic Pseudomonas aeruginosa at pH 6.5, Likely Representing Chronic, Late-Stage Cystic Fibrosis Airway Conditions .

Determination of differentially expressed peptide peak areas. The peak at m/z 579.31, corresponding to the constitutively expressed tryptic peptide AQAAEIVEQAK from the P. aeruginosa ATP synthase β-subunit, was observed at approximately the same level in each of the four analyses (anaerobic [A and B] and aerobic [C and D]). Since the peak areas varied maximally by a factor of 4, a 10-fold change in area was used in the subsequent determination of differentially expressed m/z values. The peak shown at m/z 488.77 represents one such differentially expressed peptide m/z ratio. Note the consistency of the chromatographic elution profiles that easily permits alignment of the proper peaks.

Mark D. Platt, et al. J Bacteriol. 2008 April;190(8):2739-2758.
5.
FIG. 5.

FIG. 5. From: Proteomic, Microarray, and Signature-Tagged Mutagenesis Analyses of Anaerobic Pseudomonas aeruginosa at pH 6.5, Likely Representing Chronic, Late-Stage Cystic Fibrosis Airway Conditions .

Schematic depiction of genes whose transcription is either activated anaerobically (x-fold activation [in green]) or repressed/not activated (in red) in the presence of NO3 or NO2. The boxes that are empty represent those whose cutoff values were below the designated detection limit of Tables S1 to S4 in the supplemental material. The figure includes genes involved in the transcription of nar genes (A), nir/nor genes (B), NADH dehydrogenase genes (nuo) (C), and two different predicted operons encoding PF1 bacteriophages (D and E) and TCA cycle genes that were repressed when organisms were grown in the presence of NO2 (in red) (F).

Mark D. Platt, et al. J Bacteriol. 2008 April;190(8):2739-2758.

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