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1.
Figure 5.

Figure 5. From: Locus-specific and activity-independent gene repositioning during early tumorigenesis.

Loci-specific gene repositioning during early breast tumorigenesis. (A–K) Quantitation of the radial distribution (RRD) of 11 cancer-associated genes grown in 3D culture for 20 d with 10 d of constitutive activation of ErbB2 (Day 20, ErbB2) or without activation (Day 20 control). Pairwise comparisons of cumulative radial distributions were performed using a 1D KS test. n = 195–220 per BAC per growth condition.

Karen J. Meaburn, et al. J Cell Biol. 2008 January 14;180(1):39-50.
2.
Figure 6.

Figure 6. From: Locus-specific and activity-independent gene repositioning during early tumorigenesis.

Quantitative RT-PCR analysis during differentiation of MECs. (A) Total RNA was harvested from cells grown as standard 2D cultures (Day 0), quiescent 2D cultures (Quiescent), and cultures grown for 20 d under 3D growth conditions with 10 d of constitutive activation of ErbB2 (Day 20, ErbB2) or without activation (Day 20 control). Values represent means ± SEM from three independent experiments. Expression levels are normalized to cyclophilin. (B) Pairwise comparisons for expression using a t test.

Karen J. Meaburn, et al. J Cell Biol. 2008 January 14;180(1):39-50.
3.
Figure 4.

Figure 4. From: Locus-specific and activity-independent gene repositioning during early tumorigenesis.

Quantitation of the radial distribution of specific loci during tumorigenic differentiation. (A–K) FISH was performed on PFA-fixed cells grown in 3D culture for 20 d with constitutive activation of ErbB2 for the final 10 d (Day 20, ErbB2). The radial distributions (RRD) for each gene in proliferating 2D cultures are shown for comparison (Day 0). Pairwise comparisons of cumulative radial distributions were performed using a 1D KS test. n = 195–220 per BAC per growth condition.

Karen J. Meaburn, et al. J Cell Biol. 2008 January 14;180(1):39-50.
4.
Figure 2.

Figure 2. From: Locus-specific and activity-independent gene repositioning during early tumorigenesis.

Gene positioning during normal differentiation and early mammary tumorigenesis. Indicated gene loci (red) were detected in PFA-fixed, undifferentiated, proliferating 2D culture cells (Day 0) and cells grown for 20 d under 3D growth conditions with 10 d of constitutive activation of ErbB2 (Day 20, ErbB2) or without activation (Day 20 control). Projected stacks of nuclei are shown. Note the difference in scale for Day 0 compared with 3D cultured cells. Bars, 5 μm.

Karen J. Meaburn, et al. J Cell Biol. 2008 January 14;180(1):39-50.
5.
Figure 3.

Figure 3. From: Locus-specific and activity-independent gene repositioning during early tumorigenesis.

Radial distribution of genes during differentiation of MECs. Gene loci were detected in PFA-fixed cells using specific BAC FISH probes (Table I). (A–K) Cumulative frequency graphs to quantify the radial distribution (RRD) for each gene in MCF-10.B2 cells, in proliferating 2D culture nuclei (Day 0), after growth for 20 d under 3D culture conditions (Day 20 control), and in quiescent 2D cultures (Quiescent). (L) Pairwise comparisons of cumulative radial distributions using a 1D KS test. n = 195–220 per BAC per growth condition.

Karen J. Meaburn, et al. J Cell Biol. 2008 January 14;180(1):39-50.
6.
Figure 1.

Figure 1. From: Locus-specific and activity-independent gene repositioning during early tumorigenesis.

The nucleolus during mammary epithelial differentiation and early mammary tumorigenesis. (A) MCF-10.B2 cells were stained with ANA-N antibody to detect nucleoli (green) and counterstained with DAPI to delineate nuclei (blue). Projected image stacks are shown. For acini structures, projected stacks are approximately one nucleus thick and taken from the midsection of the acini. Bar, 10 μm. (B) Quantitation of nucleoli in proliferating 2D culture (Day 0), quiescent 2D cultures (Quiescent), and cells grown for 20 d under 3D growth conditions with 10 d of constitutive activation of ErbB2 (Day 20, ErbB2) or without activation (Day 20 control). Values represent means ± SEM from a minimum of three independent experiments. n = 270–1,000.

Karen J. Meaburn, et al. J Cell Biol. 2008 January 14;180(1):39-50.

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