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Results: 4

1.
Fig. 2.

Fig. 2. From: Three-dimensional cellular microarray for high-throughput toxicology assays.

Schematic of the DataChip platform for direct testing of compound toxicity or coupling with the MetaChip for evaluating toxicity of P450-generated metabolites.

Moo-Yeal Lee, et al. Proc Natl Acad Sci U S A. 2008 January 8;105(1):59-63.
2.
Fig. 4.

Fig. 4. From: Three-dimensional cellular microarray for high-throughput toxicology assays.

Dose–response curves for Hep3B DataChip after stamping with CYP3A4 MetaChip exposed to cyclophosphamide: cyclophosphamide alone (●) and cyclophosphamide supplemented with 3.2 μM ketoconazole (▴).

Moo-Yeal Lee, et al. Proc Natl Acad Sci U S A. 2008 January 8;105(1):59-63.
3.
Fig. 3.

Fig. 3. From: Three-dimensional cellular microarray for high-throughput toxicology assays.

Results from operation of the MetaChip/DataChip platform with Hep3B cells. (A) The 45 regions are divided into five rows of miniarrays. From top to bottom: equimolar mixture of CYP3A4, CYP1A2, and CYP2D6; CYP3A4, CYP2D6, CYP1A2, and no-P450. Dose responses corresponding to the fluorescent live-cell staining are shown for four of the nine compounds tested on the chip. (B) Comparison of IC50 values determined on the chip.

Moo-Yeal Lee, et al. Proc Natl Acad Sci U S A. 2008 January 8;105(1):59-63.
4.
Fig. 1.

Fig. 1. From: Three-dimensional cellular microarray for high-throughput toxicology assays.

Preparation and characterization of the DataChip. (A) Chemical modification of a glass slide with 3-(aminopropyl) trimethoxysilane (APTMS) and poly(styrene-co-maleic anhydride) (PS-MA). (B) Construction of the collagen-gel spot containing MCF7 cells on the PS-MA-treated slide. (C) Presented left to right, microscopic photographs of collagen bottom layer (30 nl, 560 spots) on the PS-MA-treated slide, the collagen-gel spots containing MCF7 cells (60 nl, 560 spots) applied on top of the bottom layer, and live-cell stain of the array. (D) Growth of MCF7 cells in collagen-gel spots (60 nl, initial seeding of 1 × 106 cells per milliliter) based on live-cell staining at indicated incubation times.

Moo-Yeal Lee, et al. Proc Natl Acad Sci U S A. 2008 January 8;105(1):59-63.

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