We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 3

1.
Fig. 1.

Fig. 1. From: Profiling of UV-induced ATM/ATR signaling pathways.

Semiquantitative analysis of control vs. UV-damaged M059K cells reveals diversity of known/previously undescribed substrates. Pie chart shows protein classes identified in the semiquantitative analysis. All classes not shown have been collapsed into the “miscellaneous” category. The percentage of the total for each protein class is shown.

Matthew P. Stokes, et al. Proc Natl Acad Sci U S A. 2007 December 11;104(50):19855-19860.
2.
Fig. 2.

Fig. 2. From: Profiling of UV-induced ATM/ATR signaling pathways.

Western blotting and IP/Western blotting confirm phosphorylation of known/previously undescribed substrates of ATM/R seen in immunoaffinity purification/LC-MS/MS analysis. (A) Untreated (C) or UV-treated (UV) M059K lysates were subjected to Western blotting with the antibody indicated. (B) Proteins were immunoprecipitated from untreated or UV-treated M059K cell lysates with ATM/R substrate motif antibody-1 (53BP1, RPA1, and Mre11), or antibody-2 (BAP1) and blotted with the indicated antibody. “Supe” denotes post-IP supernatants; “Eluate” denotes IP elutions. For clarity, an arrow is shown to denote the band of interest in the Mre11 blot, whereas the asterisk (*) denotes a contaminating band.

Matthew P. Stokes, et al. Proc Natl Acad Sci U S A. 2007 December 11;104(50):19855-19860.
3.
Fig. 3.

Fig. 3. From: Profiling of UV-induced ATM/ATR signaling pathways.

Characterization of the DNA damage response in a Seckel cell line compared with a matched control/confirmation of immunoaffinity purification/LC-MS/MS results. (A) GM00200-matched control cells (Control) or GM18366 Seckel syndrome cells (Seckel) were untreated (C) or UV-treated (UV) and blotted with the indicated antibody. (B) Control or Seckel cells were untreated (C) or UV-treated (UV) and blotted with the indicated antibody against sites found in the semiquantitative analysis. ATM/R motif antibody-2 was shown to detect phosphorylated DNA-PK and valosin-containing protein (VCP) (17).

Matthew P. Stokes, et al. Proc Natl Acad Sci U S A. 2007 December 11;104(50):19855-19860.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk