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Results: 5

1.
FIG. 2.

FIG. 2. From: A Single-Amino-Acid Substitution in the NS1 Protein Changes the Pathogenicity of H5N1 Avian Influenza Viruses in Mice .

Comparison of weight changes in mice infected with different H5N1 avian influenza viruses. Mice (five mice/group) were intranasally infected with 106 EID50 of virus. (A) Mice infected with R-DK/12 and the reassortants in the DK/12 background. (B) Mice infected with R-DK/27 and the reassortants in the DK/27 background.

Peirong Jiao, et al. J Virol. 2008 February;82(3):1146-1154.
2.
FIG. 5.

FIG. 5. From: A Single-Amino-Acid Substitution in the NS1 Protein Changes the Pathogenicity of H5N1 Avian Influenza Viruses in Mice .

Prevention of poly(I:C)-induced activation of an NF-κB promoter and the IRF-3-depedent promoter by NS1 protein. (A) NF-κB promoter assay. 293T cells were cotransfected with pNF-κB-Luc and pTK-RL plasmids along with the specified NS1 plasmids, with or without subsequent poly(I:C) transfection. (B) ISG-54 promoter assay. Vero cells were cotransfected with pISG-54-Luc and pTK-RL plasmids along with the specified NS1 plasmids, with or without subsequent poly(I:C) transfection. Bars: 1, pCAGGS; 2, pCAGGS with poly(I:C); 3, p12NS1 with poly(I:C); 4, p27NS1 with poly(I:C); 5, p12NS1P42S with poly(I:C); 6, p27NS1S42P with poly(I:C).

Peirong Jiao, et al. J Virol. 2008 February;82(3):1146-1154.
3.
FIG. 3.

FIG. 3. From: A Single-Amino-Acid Substitution in the NS1 Protein Changes the Pathogenicity of H5N1 Avian Influenza Viruses in Mice .

NS1 mutant viruses and their virulences in mice. The color of the bar indicates the origin of the gene as follows: blue, DK/12; red, DK/27. The corresponding amino acids are shown as single-letter abbreviations with the positions numbered at the top. The red dots in the mouse figures indicate tissue tropism (upper left, brain; lower left, lung; upper right, kidney; lower right, spleen). The mutated amino acids are shown in red or blue and italics. Amino acid abbreviations: R, Arg; K, Lys; P, Pro; S, Ser; A, Ala.

Peirong Jiao, et al. J Virol. 2008 February;82(3):1146-1154.
4.
FIG. 1.

FIG. 1. From: A Single-Amino-Acid Substitution in the NS1 Protein Changes the Pathogenicity of H5N1 Avian Influenza Viruses in Mice .

Replication and lethality of the DK/12 and DK/27 viruses in mice. (A) Six-week-old SPF BALB/c mice (three/group) were inoculated intranasally with 106 EID50 of each virus in a 50-μl volume and killed on day 3 p.i., and organs were collected for virus titration in eggs. Data shown are the mean virus titers ± standard deviation. (B to E) Death patterns of the mice infected with different H5N1 viruses, DK/12 (B), DK/27(C), R-DK/12 (D), and R-DK/27 (E), with the doses of 101 to 106 EID50 (101 EID50, •; 102 EID50, ○; 103 EID50, ▵; 104 EID50, ▴; 105 EID50, ⋄; 106 EID50, ⧫).

Peirong Jiao, et al. J Virol. 2008 February;82(3):1146-1154.
5.
FIG. 4.

FIG. 4. From: A Single-Amino-Acid Substitution in the NS1 Protein Changes the Pathogenicity of H5N1 Avian Influenza Viruses in Mice .

Induction of IFN-α/β synthesis in A549 cells infected with influenza A viruses expressing wild-type or mutant NS1 proteins. (A) IFN-α/β bioassay. A549 cells were pretreated for 24 h with UV-inactivated supernatants from A549 cells infected with the indicated influenza viruses. The pretreated A549 cells were then infected with VSV-GFP, and, 14 h p.i., the cells expressing GFP were monitored by fluorescence microscopy. (B) RT-PCR analysis of IFN-α/β mRNA levels in virus-infected A549 cells. Cells were infected at an MOI of 2, and, 20 h p.i., total RNA was extracted and RT-PCR was done using primer pairs specific for human IFN-α/β and β-actin mRNA. The fragments of IFN-α, IFN-β, and β-actin are shown on the left. (C) Quantification of IFN-α/β production. A549 cells were either mock infected or infected at an MOI of 2. Twenty-four hours postinfection, the amounts of IFN-α and IFN-β released into the culture supernatant were measured by ELISA. (D) Levels of virus protein expression in infected cells. A549 cells were infected with virus at an MOI of 2 and lysed 12 h postinfection. Lysates of mock-infected cells or of cells infected with the indicated influenza viruses were incubated with mouse anti-truncated NS1 antiserum (I) or with chicken antiserum that was generated by inoculating SPF chickens with inactivated GS/GD/1/96 virus (II). Expression of β-actin protein was also examined as a control (III). Binding was visualized with DAB (3,3′-diaminobenzidine) reagent after incubation with peroxidase-conjugated secondary antibodies. The NS1, NP, and β-actin proteins are indicated on the right. Numbers 1 to 5 in panels B, C, and D indicate the type of infection as follows: 1, mock; 2, R-DK/27; 3, R-DK/12; 4, DK/27NS1S42P; 5, DK/12NS1P42S.

Peirong Jiao, et al. J Virol. 2008 February;82(3):1146-1154.

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