Results: 5

1.
Figure 5.

Figure 5. From: Mast cell deficiency in KitW-sh mice does not impair antibody-mediated arthritis.

Anti-collagen/LPS-induced joint swelling in KitW-sh mice sufficient and deficient in LILRB4. Mice were injected and their clinical scores were determined as described in the legend for Fig. 1, except that 1 mg of anti-collagen was used. Data are expressed as mean ± SEM; n = 9 from three experiments.

Joseph S. Zhou, et al. J Exp Med. 2007 November 26;204(12):2797-2802.
2.
Figure 2.

Figure 2. From: Mast cell deficiency in KitW-sh mice does not impair antibody-mediated arthritis.

IgE-PCA in mast cell–deficient and –sufficient mice. Mice were injected i.d. in one ear or s.c. in one paw with 25 and 75 ng of mouse monoclonal IgE anti-DNP, respectively, and were injected in the other ear or paw with saline. After 20 h, the mice were injected i.v. with 100 μg DNP-HSA/1% Evans blue. After 0.5 h, mice were killed, their ears and paws were excised, and the Evans blue was extracted with formamide and quantified by spectrophotometry. Data are expressed as mean ± SEM; n = 7–9 from two experiments (ear) and n = 5 from three experiments (paw).

Joseph S. Zhou, et al. J Exp Med. 2007 November 26;204(12):2797-2802.
3.
Figure 3.

Figure 3. From: Mast cell deficiency in KitW-sh mice does not impair antibody-mediated arthritis.

Peripheral blood neutrophil concentrations in naive and LPS-treated mast cell–deficient and –sufficient mice. Blood samples were obtained from KitW/KitW-v and KitW-sh mice and their respective control strains before and 24 h after i.p. injection of saline or 25 μg LPS. The concentration of neutrophils in the samples was quantified with an automated cell counter. Data are expressed as mean ± SEM; n = 7–15 per genotype.

Joseph S. Zhou, et al. J Exp Med. 2007 November 26;204(12):2797-2802.
4.
Figure 4.

Figure 4. From: Mast cell deficiency in KitW-sh mice does not impair antibody-mediated arthritis.

Histologic and flow cytometric assessments of myeloid hyperplasia in the spleen of KitW-sh mice. (A) Tissue sections from naive mice and mice on day 7 after injection of anti-collagen/LPS were stained for CAE activity and counterstained with hematoxylin. The number of CAE+ cells in the subcapsular region was counted in five 40× fields per sample. Data are expressed as mean ± SEM; n = 5 (naive) and n = 6 (day 7). (B) Spleen cells were stained with PE-labeled anti–Gr-1 or an isotype-matched negative control mAb, and fluorescence was analyzed by flow cytometry.

Joseph S. Zhou, et al. J Exp Med. 2007 November 26;204(12):2797-2802.
5.
Figure 1.

Figure 1. From: Mast cell deficiency in KitW-sh mice does not impair antibody-mediated arthritis.

Anti-collagen/LPS-induced joint swelling in KitW-sh and KitW/KitW-v mice and their respective WT strains. Mice were injected i.v. with 2 mg of a mixture of four anti-collagen mAbs on day 0 and i.p. with 25 (A and B) or 50 (B) μg LPS on day 3. A score reflecting visual assessment of joint swelling in the paw, wrist, and ankle (described in Materials and methods) of each leg was assigned, and the four scores for each mouse were summed to yield the clinical score. Data are expressed as mean ± SEM; n = 6 from two experiments (days 0–7), n = 3 from one experiment (days 8–14) (A), n = 4 from one experiment with 25 μg LPS, and n = 4 from one experiment with 50 μg LPS (B).

Joseph S. Zhou, et al. J Exp Med. 2007 November 26;204(12):2797-2802.

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