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1.
Figure 6

Figure 6. From: Requirement for the POZ/BTB protein NAC1 in acute but not chronic psychomotor stimulant response.

A. Nac1 mutant mice exhibit significantly decreased levels of dopamine in the nucleus accumbens following an acute injection of cocaine (20 mg/kg). Following the first injection, mutant mice show significantly reduced levels of dopamine in the nucleus accumbens as compared to their wild-type counterparts. There are no differences in the levels of dopamine in the nucleus accumbens of Nac1 mutant and wild-type mice following the last injection of cocaine. *p < 0.05 from mutant group (n = 6−9). B. Nac1 mutant mice and wild-type mice were exposed to 5 injections of cocaine over the course of 5 days. Both groups show equivalent increases in dopamine over the time-course examined.

Scott Mackler, et al. Behav Brain Res. ;187(1):48-55.
2.
Figure 2

Figure 2. From: Requirement for the POZ/BTB protein NAC1 in acute but not chronic psychomotor stimulant response.

A. Nac1 mutant mice do not show a significant increase in ambulatory behavior at 5mg/kg or 10mg/kg acute cocaine administration (i.p. injection), but do show a significant increase at 20mg/kg. Wild-type mice show a significantly increased behavioral response in response to all doses of acute cocaine administration. *p < 0.05 from corresponding saline group (n = 6 per group). (ANOVA; Fisher's PLSD post hoc test). B. Nac1 mutant mice do not show a significant increase in ambulatory behavior while wild-type mice do show a significantly increased behavioral response in response to acute amphetamine administration (2mg/kg i.p. injection). *p < 0.05 from corresponding saline group (n = 6 per group). (ANOVA; Fisher's PLSD post hoc test).

Scott Mackler, et al. Behav Brain Res. ;187(1):48-55.
3.
Figure 4

Figure 4. From: Requirement for the POZ/BTB protein NAC1 in acute but not chronic psychomotor stimulant response.

A. Nac1 mutant mice and wild-type mice exhibit significant cocaine-conditioned place preference. The left side of the graph indicates that no initial preference for either side of the testing box exists for any of the groups. The right side of the graph shows that both mutant and wild-type mice show a preference for the side of the testing box that was paired with 10 mg/kg cocaine. There is no significant genotype effect * p < 0.05 from corresponding saline group (n = 6 per group). (ANOVA; Fisher's PLSD post hoc test). B. Nac1 mutant mice and wild-type mice exhibit significant amphetamine-conditioned place preference. The left side of the graph indicates that no initial preference for either side of the testing box exists for any of the groups. The right side of the graph shows that both mutant mice and wild-type mice show a preference for the side of the testing box that was paired with 2 mg/kg amphetamine. There is no significant difference between the amphetamine-treated Nac1 mutant mice and the wild-type mice. * p < 0.05 from corresponding saline group (n = 6 per group). (ANOVA; Fisher's PLSD post hoc test).

Scott Mackler, et al. Behav Brain Res. ;187(1):48-55.
4.
Figure 5

Figure 5. From: Requirement for the POZ/BTB protein NAC1 in acute but not chronic psychomotor stimulant response.

A. Nac1 mutant mice do not exhibit significant one-day cocaine-conditioned place preference. The left side of the graph indicates that no initial preference for either side of the testing box exists for any of the groups. The right side of the graph shows that wild-type mice show a preference for the side of the testing box that was paired with a single injection of 20 mg/kg cocaine whereas the mutant mice do not show a significant preference. There is a significant difference between the cocaine-paired wild-type mice and the cocaine-paired Nac1 mutant mice. * p < 0.05 from all groups (n = 6 per group). (ANOVA; Fisher's PLSD post hoc test). B. Nac1 mutant mice do not exhibit significant one-day amphetamine-conditioned place preference. The left side of the graph indicates that no initial preference for either side of the testing box exists for any of the groups. The right side of the graph shows that wild-type mice show a preference for the side of the testing box that was paired with a single injection of 1 mg/kg amphetamine whereas the mutant mice do not show a significant preference. There is a significant difference between the amphetamine-paired wild-type mice and the amphetamine-paired Nac1 mutant mice. * p < 0.05 from all groups (n = 6 per group) (ANOVA; Fisher's PLSD post hoc test).

Scott Mackler, et al. Behav Brain Res. ;187(1):48-55.
5.
Figure 1

Figure 1. From: Requirement for the POZ/BTB protein NAC1 in acute but not chronic psychomotor stimulant response.

Generation of Nac1 mutant mice. A. The targeting vector of Nac1 a 5kb lacZ-PGK-neo cassette was inserted into exon 2; Enzyme restriction sites are designated as X;Xba and E; EagI, the positions of primersets used to detect wild-type and mutant alleles are indicated by black arrows, geneotypes of mice were identified using PCR primers corresponding to 5' and 3' in wild type allele, where the 3' primer is located just downstream of the EagI site where the targeting vector was inserted. B. DNA analysis in wild type and mutant mice. Wild-type band is 293bp, mutant band 340bp that is amplified using the same 5'primer and a primer located within the lacZ-PGK-neo cassette. C. The absence of Nac1 mRNA was confirmed by RT-PCR analysis using primer sets corresponding to either the POZ/BTB domain or a 5' region of Exon 1. HPRT is used as an internal control. D. The absence of Nac1 protein was confirmed in mutant mice by Western analysis in both nucleus accumbens (NAC-/-) and cortex (CTX-/-).

Scott Mackler, et al. Behav Brain Res. ;187(1):48-55.
6.
Figure 3

Figure 3. From: Requirement for the POZ/BTB protein NAC1 in acute but not chronic psychomotor stimulant response.

A. Behavioral sensitization in Nac1 mutant and wild-type mice. No significant differences between the wild type and mutant mice during the habituation period (days 1−3; saline injections only). A geneotype effect is present on the first day of cocaine administration (day 4). During the development of sensitization (days 5−10) cocaine-treated (10mg/kg i.p. injection) wild-type mice and Nac1 mutant mice exhibit significantly more ambulatory activity than saline-treated mice Both cocaine-treated groups exhibit sensitization on the challenge days (days 29 and 40; 5mg/kg cocaine). # p < 0.05 from corresponding saline group on same day, and * p < 0.05 from corresponding group on day 4 (n = 6 per group). (ANOVA; Fisher's PLSD post hoc test). B. Behavioral sensitization to amphetamine (2 mg/kg) in Nac1 mutant mice (NAC1−/−) and wild-type mice. No significant differences appear between geneotypes during the habituation period (days 1−3; saline injections only). During the development of sensitization (days 4−7) amphetamine-treated mice exhibit significantly more ambulatory activity than saline-treated mice in all cases except for Nac1 mutant mice on the first and second days of development (days 4 and 5). Both groups show no difference in ambulation in response to saline after development of sensitization (day 90) and both amphetamine-treated groups exhibit sensitization on the challenge days (day 91; 2mg/kg amphetamine). # p < 0.05 from corresponding saline group on same day, * p < 0.05 and + p < 0.01 from corresponding group on day 90 (n = 6 per group). (ANOVA; Fisher's PLSD post hoc test).

Scott Mackler, et al. Behav Brain Res. ;187(1):48-55.

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