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Results: 5

1.
Figure 4

Figure 4. From: Differentiation, Survival, and Function of Embryonic Stem Cell-Derived Endothelial Cells for Ischemic Heart Disease.

Molecular imaging of ESC-EC fate after transplantation. (a) A representative animal injected with 5x105 ESC-ECs shows significant bioluminescence activity at day 2 which decreases progressively over the following 8 weeks. Control animal injected with PBS show no imaging signals as expected. (b) Detailed quantitative analysis of signals from all animals transplanted with ESC-ECs (signal activity is expressed as photons/sec/cm2/sr). (c) Donor cell survival plotted as % signal activity from day 2 to week 8.

Zongjin Li, et al. Circulation. ;116(11 0):I46-I54.
2.
Figure 5

Figure 5. From: Differentiation, Survival, and Function of Embryonic Stem Cell-Derived Endothelial Cells for Ischemic Heart Disease.

Functional evaluation of transplanted ESC-ECs. (a) Representative M-mode still frames of control and ESC-EC animals at day 2 and week 8 after LAD ligation. Echocardiographic data comparing fractional shortening (FS) between the two groups 2 days before (pre), 2 days after, and 8 weeks after LD ligation. *P<0.05 compared with control; #P<0.01 compared with day 2 (n=10 in all group). (b) Quantitative analysis of capillary density was significantly higher in the ESC-EC group compared to the control group (200x magnification).

Zongjin Li, et al. Circulation. ;116(11 0):I46-I54.
3.
Figure 1

Figure 1. From: Differentiation, Survival, and Function of Embryonic Stem Cell-Derived Endothelial Cells for Ischemic Heart Disease.

ES cell differentiation into endothelial-like cells. (a) Schema of the endothelial cell lineage-specific promoter with VE-cadherin promoter driving eGFP and SV40 promoter driving neomycin resistant gene for antibiotic selection. (b) After 4 days on collagen IV plates, Flk-1+ positive cells can be identified by FACS and brightfield microscopy (100x magnification). (c) After another 4 days of culturing on differentiation medium, ~36% of cells express both VE- cadherin surface marker and intracellular eGFP. These double positive cells show typical endothelial cell morphology and can be expanded for additional 5–8 passages (100x magnification).

Zongjin Li, et al. Circulation. ;116(11 0):I46-I54.
4.

Figure 2. From: Differentiation, Survival, and Function of Embryonic Stem Cell-Derived Endothelial Cells for Ischemic Heart Disease.

In vitro characterization of ESC-ECs. (a) FACS analysis of common endothelial markers in undifferentiated ES cells and differentiated ESC-ECs. (b) Uptake of Dil-ac-LDL by ESC-ECs. (c) Vasculogenic formation by ESC-ECs after 24 and 48 hours of plating. Undifferentiated ES cells do not form cord-like structures (100x magnification). (d) Expression profile of endothelial cell-specific genes at different time points: undifferentiated ES cells (day 0), Flk-1+ cells (day 4), and VE-cadherin+/eGFP+ cells (day 8). The positive control is adult mouse endothelial cells (mEC) from the lung microvasculature. The negative control has no PCR templates.

Zongjin Li, et al. Circulation. ;116(11 0):I46-I54.
5.
Figure 3

Figure 3. From: Differentiation, Survival, and Function of Embryonic Stem Cell-Derived Endothelial Cells for Ischemic Heart Disease.

Transduction of ES cells with double fusion reporter genes. (a) Schema of the DF reporter gene with ubiquitin promoter driving Fluc and mRFP joined by a 20-amino acid linker. (b) FACS analysis showing lentiviral transduction efficiency (~21.4%) after 48 hours. Isolated cells are strongly positive for mRFP on fluorescence microscopy. (c) Ex vivo imaging analysis of transduced cells show increasing bioluminescence signals with cell numbers (r2=0.98). (d) In vitro enzyme assay show increasing Fluc enzyme activity with cell numbers (r2=0.94).

Zongjin Li, et al. Circulation. ;116(11 0):I46-I54.

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