Display Settings:

Items per page

Results: 7

1.
Figure 5

Figure 5. RARγ-/- Mice Have a Microenvironment-Induced Myeloproliferative Syndrome. From: A Microenvironment-Induced Myeloproliferative Syndrome Caused by RAR? Deficiency.

(A-B) Peripheral blood (A) leukocyte and (B) granulocyte counts in 8 week old RARγ+/+ and RARγ-/- mice.
(C-D) Peripheral blood (C) leukocyte and (D) granulocyte counts in CD45.1+ congenic recipients transplanted with RARγ+/+ or RARγ-/- BM, 8 weeks post-transplant.
(E-F) Peripheral blood (E) leukocyte and (F) granulocyte counts in RARγ+/+ or RARγ-/- recipients transplanted with CD45.1+ congenic BM, 5 weeks post-transplant.
(G) B lymphocyte and erythroid content in BM of RARγ+/+ or RARγ-/- recipients transplanted with RARγ+/+ or RARγ-/- BM, 5-8 weeks post-transplant.
Results are expressed as mean ± SEM, n=6 (A,B,G), n=4-6 (C-F, G). *P<0.05, #P<0.005 compared to RARγ+/+ (Student’s t-test).

Carl R. Walkley, et al. Cell. ;129(6):1097-1110.
2.
Figure 4

Figure 4. Ageing RARγ-/- Mice Exhibit a Profound Myeloproliferative-Like Disease. Representative sections of organs from 9-12 month old RARγ+/+ or RARγ-/- mice. From: A Microenvironment-Induced Myeloproliferative Syndrome Caused by RAR? Deficiency.

(A-B) Hematoxylin and eosin stained sections of (A) RARγ+/+ or (B) RARγ-/- BM. Original magnification × 4. Identified are: trabecular bone (large arrows), cortical bone (arrowheads).
(C-D) Hematoxylin and eosin stained sections of (C) RARγ+/+ or (D) RARγ-/- BM. Original magnification × 100. Identified are: megakaryocytes (large arrows), granulocytes (arrowheads).
(E-F) Hematoxylin and eosin stained sections of (E) RARγ+/+ or (F) RARγ-/- adipose tissue. Original magnification × 20.
(G) Higher power magnification of a section of RARγ-/- adipose tissue, stained with hematoxylin and eosin. Original magnification × 100. Identified are: megakaryocytes (large arrows), monocytes (double-headed arrows), erythroid cells (short arrows) and granulocytes (arrowheads).
(H) Myeloperoxidase (MPO) stained section of RARγ-/- adipose tissue. Original magnification × 40. MPO-positive cells are brown.

Carl R. Walkley, et al. Cell. ;129(6):1097-1110.
3.
Figure 3

Figure 3. 12 Month Old RARγ-/- Mice Have Profoundly Elevated Numbers of Mature and Immature Granulocytes and Progenitor Cells. From: A Microenvironment-Induced Myeloproliferative Syndrome Caused by RAR? Deficiency.

(A) Numbers of PB granulocytes in 12 month old RARγ+/+ or RARγ-/- mice.
(B) Numbers of immature and mature granulocytes in 12 month old RAR+/+ or RARγ-/- BM.
(C) Numbers of granulocytes in 12 month old RARγ+/+ or RARγ-/- spleen.
(D) Numbers of 12 day CFU-GEMM formed per 1 × 105 RARγ+/+ or RARγ-/- PB cells.
(E) Numbers of 7 day GM-CSF- or SCF + G-CSF-responsive colony-forming cells formed per 5 × 104 RARγ+/+ or RARγ-/- BM cells.
(F) Numbers of 12 day CFU-GEMM formed per 1 × 105 RARγ+/+ or RARγ-/- spleen (Sp) cells.
(G) Numbers of 12 day CFU-GM formed per 1 × 105 RARγ+/+ or RARγ-/- PB cells.
(H) Numbers of 7 day G-CSF-responsive colony-forming cells generated per 5 × 104 RARγ+/+ or RARγ-/- BM cells.
(I) Numbers of 12 day CFU-GM formed per 1 × 105 RARγ+/+ or RARγ-/- spleen (Sp) cells.
(J) B lymphocyte and erythroid content in BM of 12 month old RARγ+/+ or RARγ-/- mice.
Results are expressed as mean ± SEM, n=4-8 (A-C, J), n=3 (D-I). *P<0.05, #P<0.005 compared to RARγ+/+ (Student’s t-test).

Carl R. Walkley, et al. Cell. ;129(6):1097-1110.
4.
Figure 1

Figure 1. 8 week old RARγ-/- Mice have Elevated Numbers of Granulocytes. From: A Microenvironment-Induced Myeloproliferative Syndrome Caused by RAR? Deficiency.

(A) Leukocyte cellularity in peripheral blood (PB), bone marrow (BM), spleen and thymus, in addition to body weights of 8 week old RARγ mutant mice.
(B-D) Flow cytometric analysis of granulocytes and absolute numbers of granulocyte populations in 8 week old RARγ mutant mice:
(B) Representative PB granulocyte FACS profiles, with the absolute numbers of granulocytes (× 103 cells/μl blood) shown to the right of each FACS profile.
(C) Representative BM granulocyte FACS profiles, with the absolute numbers of immature granulocytes (Gr-1dim) and mature granulocytes (Gr-1bright; × 106 cells/femur) shown at the bottom of each FACS profile.
(D) Representative spleen granulocyte FACS profiles, with the absolute numbers of granulocytes (× 107 cells/spleen) shown to the right of each FACS profile.
Data are expressed as the mean ± SEM (n=6-9 per genotype). *P<0.05; #P<0.005 compared to RARγ+/+ mice (Student’s t-test).

Carl R. Walkley, et al. Cell. ;129(6):1097-1110.
5.
Figure 6

Figure 6. The Microenvironment-Induced Myeloproliferative Syndrome Observed in RARγ-/- Mice is Partially Due to Increased TNFα Signaling. From: A Microenvironment-Induced Myeloproliferative Syndrome Caused by RAR? Deficiency.

(A) Relative expression of TNFα in cDNA from bone marrow, spleen and thymus extracts from 8 week old RARγ+/+ and RARγ-/- mice.
(B-C) Fold-differences observed in peripheral blood (A) leukocyte and (B) granulocyte counts when TNFα+/+ or TNFα-/- BM was transplanted into RARγ+/+ compared to RARγ-/- recipient mice.
(D-E) Fold-differences observed in BM (C) immature and (D) mature granulocyte counts when TNFα+/+ or TNFα-/- BM was transplanted into RARγ+/+ compared to RARγ-/- recipient mice.
(F-G) Fold-differences observed in spleen (E) leukocyte and (F) granulocyte counts when TNFα+/+ or TNFα-/- BM was transplanted into RARγ+/+ compared to RARγ-/- recipient mice.
(H) B lymphocyte and erythroid content in BM of RARγ+/+ or RARγ-/- recipients transplanted with TNFα-/- BM.
The levels of expression of TNFα were quantified by Q-RT-PCR and are given as arbitrary units relative to β2-microglobulin. The data represent the mean ± SEM of three different samples for each genotype. #P<0.005 compared to RARγ+/+ (Student’s t-test). Numbers in parentheses indicate fold-differences in expression between RARγ+/+ and RARγ-/- organs. Transplant data are shown at 5-8 weeks post-transplant, n=4-6 recipients/group.

Carl R. Walkley, et al. Cell. ;129(6):1097-1110.
6.
Figure 7

Figure 7. Absolute Requirement for an RARγ-/- Microenvironment to Sustain the Myeloproliferative-Like Disease. From: A Microenvironment-Induced Myeloproliferative Syndrome Caused by RAR? Deficiency.

Shown are representative May-Grunwald Giemsa-stained PB smears of (A) an RARγ+/+ recipient and (B) an RARγ-/- recipient (with PB leukocyte cellularity >48 × 106 cells/ml), both transplanted with the same donor CD45.1+ congenic wildtype bone marrow. Identified are: normal granulocyte (large arrow), immature myeloid cells (arrowheads), abnormal erythrocytes (double-headed arrows).
(C-D) FACS analysis of the RARγ+/+ recipient shows high levels of donor cell reconstitution (C) and normal granulocyte profiles (D) in the PB.
(E-F) FACS analysis of the RARγ-/- recipient shows high levels of donor cell reconstitution (E) and abnormal granulocyte profiles (F) in the PB.
(G-H) Two representative FACS profiles of donor-derived PB granulocytes in secondary RARγ+/+ recipients transplanted with 2.5 × 106 spleen leukocytes obtained from the primary RARγ-/- recipient whose PB parameters are shown in 7B, E and F.
(I-J) Two representative FACS profiles of donor-derived PB granulocytes in secondary RARγ-/- recipients transplanted with 2.5 × 106 spleen leukocytes obtained from the primary RARγ-/- recipient whose PB parameters are shown in 7B, E and F.
Data are shown at 8 weeks post-transplant, n=3-5 ecipients/group

Carl R. Walkley, et al. Cell. ;129(6):1097-1110.
7.
Figure 2

Figure 2. 8 Week Old RARγ-/- Mice Have Increased Numbers of Granulocyte Progenitors. From: A Microenvironment-Induced Myeloproliferative Syndrome Caused by RAR? Deficiency.

(A) Numbers of 7 day GM-CSF- or SCF+G-CSF-responsive colony-forming cells formed per 5 × 104 RARγ+/+ or RARγ-/- BM cells.
(B) Numbers of 7 day G-CSF-responsive colony-forming cells formed per 5 × 104 RARγ+/+ or RARγ-/- BM cells.
(C) Numbers of 3 day G-CSF-responsive cluster-forming cells (Cl-FCs) formed per 5 × 104 RARγ+/+ or RARγ-/- BM cells.
(D) Numbers of 12 day CFU-GM formed per 1 × 105 RARγ+/+ or RARγ-/- PB cells.
(E) Numbers of 12 day CFU-GM formed per 1 × 105 RARγ+/+ or RARγ-/- spleen (Sp) cells.
(F) Numbers of 7 day G-CSF-responsive colony-forming cells generated per 5 × 104 RARγ+/+ or RARγ-/- BM cells in response to submaximal and supramaximal concentrations of G-CSF.
(G) Numbers of 7 day G-CSF-responsive colony-forming cells generated per 5 × 104 RARγ+/+ or RARγ-/- BM cells in response to delayed addition (24 hours or 48 hours) of G-CSF to the cultures.
(H) Frequencies of common myeloid progenitors (CMP), granulocyte macrophage progenitors (GMP) and megakaryocyte erythroid progenitors (MEPs) present in RARγ+/+ or RARγ-/- BM.
Results are expressed as mean ± SEM, n=4 (A-C), n=3 (D-H). *P<0.05, #P<0.005 compared to RARγ+/+ (Student’s t-test).

Carl R. Walkley, et al. Cell. ;129(6):1097-1110.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk