Display Settings:

Items per page

Results: 6

1.
FIG. 6.

FIG. 6. From: N-Linked Glycosylation Attenuates H3N2 Influenza Viruses .

Hemagglutination inhibition capacity of rhSP-D is inversely related to the glycosylation of viruses. Maximal hemagglutination inhibition of rhSP-D was determined by incubation of virus in the presence of 1.25 μg of rhSP-D.

David J. Vigerust, et al. J Virol. 2007 August;81(16):8593-8600.
2.
FIG. 2.

FIG. 2. From: N-Linked Glycosylation Attenuates H3N2 Influenza Viruses .

Sites of glycosylation on the influenza virus HA. Site-directed mutagenesis was used to generate additional sites for N-linked glycosylation. (A) The amino acid identity, position, and location within the globular head of H3N2 HA are indicated where additional sites of potential glycosylation were introduced. (B) To demonstrate that these sites were occupied by carbohydrate, the electrophoretic mobility of purified virus was determined by immunoprecipitation and Western blotting followed by resolution on a 10 to 20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel.

David J. Vigerust, et al. J Virol. 2007 August;81(16):8593-8600.
3.
FIG. 4.

FIG. 4. From: N-Linked Glycosylation Attenuates H3N2 Influenza Viruses .

Increasing glycosylation decreases the histopathologic damage to the lungs. Mice were infected with 1 × 106 EID50 of the HK68 WT virus (A, D, and G) the HK68 Δ248 (+3) virus (B, E, and H), or the HK68 Δ144 (+5) virus (C, F, and J). Representative sections of lungs removed 5 days after infection are shown for each virus. Panels A, B, and C show representative areas of the parenchyma (magnification, ×20); panels D, E, and F show large airways (magnification, ×20); and panels G, H, and I show the epithelial border of a bronchus (magnification, ×40).

David J. Vigerust, et al. J Virol. 2007 August;81(16):8593-8600.
4.
FIG. 5.

FIG. 5. From: N-Linked Glycosylation Attenuates H3N2 Influenza Viruses .

Mice deficient in SP-D are more susceptible to highly glycosylated viruses. Groups of 3 to 4 mice deficient for SP-D were infected with 1 × 106 EID50 of otherwise isogenic influenza viruses engineered to have additional potential sites of glycosylation and followed for weight loss (A) and survival (B). (C) Total viral lung loads were determined for mice infected with viruses for 7 days. TCID50, 50% tissue culture infective dose.

David J. Vigerust, et al. J Virol. 2007 August;81(16):8593-8600.
5.
FIG. 1.

FIG. 1. From: N-Linked Glycosylation Attenuates H3N2 Influenza Viruses .

Viruses with different HAs differ in virulence based on their level of glycosylation. Groups of 6 mice were infected with 1 × 106 EID50 of reassortant viruses expressing the HA from HK68 (7 glycosylation sites), Len86 (9 sites), or Pan99 (12 sites) paired with the NA from HK68 (A and B) or Syd97 (C and D) to determine the effect of glycosylation on morbidity (weight loss) (A and C) and survival (B and D). An asterisk denotes a statistically significant difference compared to the group infected with a virus expressing the Pan99 HA; a double asterisk indicates a significant difference compared to both other viruses.

David J. Vigerust, et al. J Virol. 2007 August;81(16):8593-8600.
6.
FIG. 3.

FIG. 3. From: N-Linked Glycosylation Attenuates H3N2 Influenza Viruses .

Increasing glycosylation of the influenza virus HA attenuates the virus. Groups of 6 mice were infected with 1 × 106 EID50 of otherwise isogenic influenza viruses engineered to have additional potential sites of glycosylation and followed for weight loss (A) and survival (B). (C) Total viral lung loads were determined for mice infected with viruses for 3 or 7 days. Significant differences are indicated as follows: *, compared to viruses with +1 to +5 additional glycosylation sites; **, compared to the viruses with +2 to +5 additional glycosylation sites; ***, compared to all other groups. TCID50, 50% tissue culture infective dose.

David J. Vigerust, et al. J Virol. 2007 August;81(16):8593-8600.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk