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1.
FIG. 1.

FIG. 1. From: Heterogeneity in the Activity of Mexican Helicobacter pylori Strains in Gastric Epithelial Cells and Its Association with Diversity in the cagA Gene .

Determination of the size of the cagA 3′-variable region by PCR. PCR products from single-colony isolates from different patients or from the same patient (259, 261, and 555) showed size variation. Lane M, molecular size marker.

Adriana Reyes-Leon, et al. Infect Immun. 2007 July;75(7):3445-3454.
2.
FIG. 6.

FIG. 6. From: Heterogeneity in the Activity of Mexican Helicobacter pylori Strains in Gastric Epithelial Cells and Its Association with Diversity in the cagA Gene .

Relationship between EPIYA patterns and the ability to induce both IL-8 secretion and cellular elongation for 30 cagPAI+ H. pylori isolates from our population. Isolates without the B motif (ACC pattern; patient 646) or with a modified B motif (EPTYA; patient 248) had a significantly weaker cellular elongation phenotype.

Adriana Reyes-Leon, et al. Infect Immun. 2007 July;75(7):3445-3454.
3.
FIG. 5.

FIG. 5. From: Heterogeneity in the Activity of Mexican Helicobacter pylori Strains in Gastric Epithelial Cells and Its Association with Diversity in the cagA Gene .

Deduced amino acid sequences of C-terminal repeat regions of CagA from cagPAI+ isolates that were positive or negative for induction of IL-8 or cellular elongation (Hm). The EPIYA-A, -B, and -C motifs and the SHP-2 binding site after the EPIYA-C motif are underlined, and the Western sequence type is shaded.

Adriana Reyes-Leon, et al. Infect Immun. 2007 July;75(7):3445-3454.
4.
FIG. 3.

FIG. 3. From: Heterogeneity in the Activity of Mexican Helicobacter pylori Strains in Gastric Epithelial Cells and Its Association with Diversity in the cagA Gene .

Diversity in the induction of cellular elongation and IL-8 secretion by multiple cagPAI+ H. pylori strains, isolates with a partial cagPAI, and cagPAI isolates from single patients. Patient numbers are indicated on the x axis, and intensities of cell activities are shown on the y axes. Horizontal lines indicate mean values, and bars indicate standard deviations. The results represent the means for three independent experiments.

Adriana Reyes-Leon, et al. Infect Immun. 2007 July;75(7):3445-3454.
5.
FIG. 4.

FIG. 4. From: Heterogeneity in the Activity of Mexican Helicobacter pylori Strains in Gastric Epithelial Cells and Its Association with Diversity in the cagA Gene .

Translocation and phosphorylation of the CagA protein in AGS cells infected with cagPAI+ and partial cagPAI-carrying H. pylori isolates. Western blot analysis of CagA translocation into AGS cells was performed with a polyclonal anti-CagA antibody, and detection of CagA phosphorylation was done with an anti-phosphotyrosine monoclonal antibody. Isolate 365a3 is a partial cagPAI-carrying (cagA negative) isolate; all other isolates (from 646a5 to 249c5) are cagPAI+. Isolate 236c6 was negative for Western blotting with anti-CagA but positive with anti-phospho-Tyr. Isolates with variability in biological activity levels are marked with circles or squares.

Adriana Reyes-Leon, et al. Infect Immun. 2007 July;75(7):3445-3454.
6.
FIG. 2.

FIG. 2. From: Heterogeneity in the Activity of Mexican Helicobacter pylori Strains in Gastric Epithelial Cells and Its Association with Diversity in the cagA Gene .

Diversity in biological activities on AGS cells of 62 cagPAI+ isolates, 4 isolates with a partial cagPAI (cagA negative), and 9 cagPAI isolates. (A) Induction of IL-8 expression. (B) Induction of cellular elongation. The intensities of both activities varied considerably among the cagPAI+ isolates and were significantly higher than those of partial cagPAI and cagPAI isolates. Horizontal lines indicate mean values. The results represent the means for three independent experiments. The cutoff values for both activities were estimated as the means plus 3 standard deviations of the values obtained with the Tx30a reference strain and nine cagPAI isolates.

Adriana Reyes-Leon, et al. Infect Immun. 2007 July;75(7):3445-3454.

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