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Results: 5

1.
FIGURE 1.

FIGURE 1. From: Elevated FMR1 mRNA in premutation carriers is due to increased transcription.

Diagram of the approximate locations of primers and probes used for the current RT-PCR analysis. Sequences of the primers and probes are given in Table 1.

Flora Tassone, et al. RNA. 2007 April;13(4):555-562.
2.
FIGURE 4.

FIGURE 4. From: Elevated FMR1 mRNA in premutation carriers is due to increased transcription.

Expansion of the CGG repeat in the FMR1 mRNA does not affect the fraction of message that possesses a 5′ cap. Capped and uncapped mRNAs were fractionated by affinity chromatography (see Materials and Methods); capped and uncapped mRNAs were quantified by TaqMan RT-PCR, using either GUS (A) or GAPDH (B) mRNAs as reference genes.

Flora Tassone, et al. RNA. 2007 April;13(4):555-562.
3.
FIGURE 3.

FIGURE 3. From: Elevated FMR1 mRNA in premutation carriers is due to increased transcription.

In situ hybridization with β-tubulin and FMR1 antisense strand in lymphoblastoid cell line derived from a normal individual (AG), from a male premutation carrier (TF, 190 CGG repeats), and from an individual with a hypermethylated full mutation (GM, ∼600 CGG repeats).

Flora Tassone, et al. RNA. 2007 April;13(4):555-562.
4.
FIGURE 5.

FIGURE 5. From: Elevated FMR1 mRNA in premutation carriers is due to increased transcription.

Graph of the relationship between relative FMR1 mRNA levels, the number of CGG repeats, and the number of AGG interruptions, for a cohort of 128 adult male premutation carriers and controls. Gray circles, 2 AGG interruptions; open circles, 1 AGG; black circles, 0 AGGs. (Inset) Histogram representing the average FMR1 mRNA levels for premutation alleles with 0 AGG interruptions or with 1 AGG interruption (includes two cases with 2 AGGs); error bars represent SEMs.

Flora Tassone, et al. RNA. 2007 April;13(4):555-562.
5.
FIGURE 2.

FIGURE 2. From: Elevated FMR1 mRNA in premutation carriers is due to increased transcription.

Relative FMR1 mRNA and transcript levels in premutation versus normal lymphoblastoid cell lines. (A) Analysis of spliced message using primers located in exons 3 and 4, with the probe spanning the intron/exon junction. (B) Analysis of pre- or partially spliced transcripts using primers located in introns 2 and 3 (MM1) or in exon 3 and intron 4 (MM2). GM, hypermethylated, full mutation allele (∼600 CGG repeats); AG, normal allele (female; 16, 29 CGG repeats); MM, large premutation allele (160 CGG repeats). Error bars reflect measurements performed in triplicate.

Flora Tassone, et al. RNA. 2007 April;13(4):555-562.

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