Results: 4

1.
Figure 4

Figure 4. Representation of the position of the position of the mgi residues in the structure of the yeast F1-ATPase. From: Mitochondrial Genome Integrity Mutations Uncouple the Yeast Saccharomyces cerevisiae ATP Synthase.

A, this is a stripped down view of the yeast F1-ATPase with the α-(salmon), β-(slate), and γ- (yellow), δ-(gold), and ∊-(purple) subunits. Only pertinentregions of the α-and β-subunits are shown. The mgi residues are colored in red, blue, and green and shown as spheres.B–D, enlargement areas of interest to illustrate the interactions of the residues in the mgi complementation group. The residues and subunits are labeled as indicated. This figure was made using PYMOL (47).

Yamin Wang, et al. J Biol Chem. ;282(11):8228-8236.
2.
Figure 2

Figure 2. Growth phenotype of the yeast strains with the mgi mutations. From: Mitochondrial Genome Integrity Mutations Uncouple the Yeast Saccharomyces cerevisiae ATP Synthase.

The wild type and mutant yeast strains were grown on YPD and YPG at 30 °C at 3 dilutions, as indicated. The haploid cells were mated to the wild type strain, K289-3A (21), and a diploid cell selected and tested for growth in the same manner. Thus, the diploid strain is heterozygous, whereas the haploid strain is homozygous, for the mgi mutation.

Yamin Wang, et al. J Biol Chem. ;282(11):8228-8236.
3.
Figure 1

Figure 1. Color sectoring of the yeast strains containing the mgi mutations. From: Mitochondrial Genome Integrity Mutations Uncouple the Yeast Saccharomyces cerevisiae ATP Synthase.

The images show yeast colonies of the wild type (WT) and mutant strains (as indicated) after growth on YPD medium. There are two image plates associated with each strain: one showing the entire culture plate and the second showing the colonies. The ade2 mutation in the strains is responsible for the red color. The absence of the red (white) is due to the petite mutation (see text). The pink color is representative of a defect in oxidative phosphorylation without loss of mitochondrial DNA.

Yamin Wang, et al. J Biol Chem. ;282(11):8228-8236.
4.
Figure 3

Figure 3. In vivo staining of the mitochondrion as a measure of the membrane potential. From: Mitochondrial Genome Integrity Mutations Uncouple the Yeast Saccharomyces cerevisiae ATP Synthase.

There are three image plates for the wild type, a mutant strain containing the αN67I mutation, and a cytoplasmic petite mutant strain. The first plate is an epifluorescence image of the cells after staining with Rhodamine 123. The second plate is a phase-contrast image of the same view. The final plate represents the data obtained after fluorescent-activated cell counting. Notice the log scale of the abscissa. In the final plate, the area under the curve with the fluorescence height is listed in Table 2, fourth column.

Yamin Wang, et al. J Biol Chem. ;282(11):8228-8236.

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