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Results: 6

1.
Fig. 2

Fig. 2. From: Selenomethionine Induced Transcriptional Programs in Human Prostate Cancer Cells.

Bar charts show biological process categories of genes affected by SM. Red lettering indicates statistically significant enrichment, as identified by GOTM. Black lettering indicates no statistically significant enrichment, as identified by GOTM. A and a, biological processes of genes up-regulated by SM. B and b, and C and c, biological processes of genes down-regulated by SM, respectively.

Hongjuan Zhao, et al. J Urol. ;177(2):743-750.
2.
Fig. 4

Fig. 4. From: Selenomethionine Induced Transcriptional Programs in Human Prostate Cancer Cells.

Real-time RT-PCR shows validation of gene expression changes using microarray. A, expression changes of 8 genes in response to MSA and SM determined by microarray and viewed in Treeview. B, expression changes of same genes in response to MSA and SM at 6 and 12 hours, respectively, determined by RT-PCR and compared with those determined by microarray analysis. Levels of transcripts determined by RT-PCR in triplicate were normalized against those of GAPDH in same sample and they are shown in same order as in part A.

Hongjuan Zhao, et al. J Urol. ;177(2):743-750.
3.
Fig. 3

Fig. 3. From: Selenomethionine Induced Transcriptional Programs in Human Prostate Cancer Cells.

Overview of 4,145 transcripts whose expression changed at least 1.5-fold compared to control in at least 3 experiments in response to MSA (A) or SM (B). Columns represent data from 1 time point after treatment. Rows represent single gene expression across time course. Red areas indicate up-regulated transcripts. Green areas represent down-regulated transcripts. Color saturation degree corresponds to gene expression ratio of treated cells vs controls (color bar, bottom right).

Hongjuan Zhao, et al. J Urol. ;177(2):743-750.
4.
Fig. 6

Fig. 6. From: Selenomethionine Induced Transcriptional Programs in Human Prostate Cancer Cells.

Androgen responsive genes modulated by SM. Genes that occur more than once are represented by multiple clones on arrays. A, SM affected transcripts present in list of androgen responsive transcripts identified by DePrimo et al.12 Left, gene expression patterns from 2 time courses induced by treatment of LNCaP with synthetic androgen R1881. Red arrowheads indicate well characterized androgen regulated genes. B, effects of SM and MSA on luciferase activity driven by PSA promoter after 24 hours of treatment normalized against luciferase activity in control cells. Asterisks indicate significantly different effects on reporter construct by selenium compounds.

Hongjuan Zhao, et al. J Urol. ;177(2):743-750.
5.
Fig. 5

Fig. 5. From: Selenomethionine Induced Transcriptional Programs in Human Prostate Cancer Cells.

SM effects on cell cycle regulated genes and cell cycle progression. A, cell cycle regulated transcripts previously identified as being down-regulated or up-regulated by SM. Right, number of transcripts belonging to different cell cycle phases and SM effect on expression of these genes, organized as in figure 1. Left, pattern of these genes across multiple cell cycles in HeLa cells. Thy-Thy indicates double thymidine block to synchronize cells at S-phase before release. Thy-Noc indicates thymidinenocodazole block to synchronize cells at mitosis before release. Green bar represents S-phase. Red arrowheads indicate mitosis, as determined by flow cytometry or bromodeoxyuridine labeling. B, LNCaP cell cycle distribution after SM treatment by flow cytometry analysis. Asterisk indicates statistically significantly different.

Hongjuan Zhao, et al. J Urol. ;177(2):743-750.
6.
Fig. 1

Fig. 1. From: Selenomethionine Induced Transcriptional Programs in Human Prostate Cancer Cells.

A, hierarchical clustering analysis of gene expression changes induced by SM in LNCaP. Data are arranged in time ascending order of 1, 2, 4, 6, 9, 12, 15, 18, 24 and 48 hours. Columns represent data from single time point after SM treatment. Rows represent single gene expression across time course (top). Red areas indicate up-regulated transcripts. Green areas indicate down-regulated transcripts. Color saturation degree corresponds to gene expression ratio (bottom). Full transcript identities and raw data are available at http://www.Stanford.edu/~hongjuan/SM. B, expression fold changes induced by SM at 1, 12 and 48 hours. Genes are in same order as in part A. C, hierarchical clustering analysis of genes with significantly higher expression in SM vs MSA treated cells, as identified by SAM analysis. Data on each treatment condition are arranged in time ascending order (top).

Hongjuan Zhao, et al. J Urol. ;177(2):743-750.

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