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Results: 4

1.
Fig.1

Fig.1. PARP inhibitors decrease VEGF- and FGF-induced proliferation of HUVECs. From: Poly(ADP-ribose) polymerase inhibition decreases angiogenesis.

(A) IQD decreases VEGF- and FGF-induced proliferation of HUVECs.
Cells were treated with VEGF or FGF 20 ng/ml alone or with VEGF/FGF + IQD as indicated and the proliferation of HUVECs was determined by measuring the amount of BrdU incorporated using ELISA kit colorimetrically at 450 nm.
(B) 5-AIQ decreases VEGF- and FGF-induced proliferation of HUVECs.
* P < 0.00001 vs. control; # P < 0.05 vs. VEGF or FGF; ‡ P < 0.01 vs. VEGF or FGF; † P< 0.001 vs. VEGF or FGF, n = 3.

Mohanraj Rajesh, et al. Biochem Biophys Res Commun. ;350(4):1056-1062.
2.
Fig.4

Fig.4. PARP inhibitors abrogate sprouting of rat aortic rings. From: Poly(ADP-ribose) polymerase inhibition decreases angiogenesis.

Representative images of rat aortic ring sprouting in response to treatments as indicated in the figure (above) and quantitative data on the sprout lengths (below).
* P < 0.00001 vs. control; # P < 0.05 vs. VEGF or FGF; ‡ P < 0.01 vs. VEGF or FGF; † P< 0.001 vs. VEGF or FGF, n = 3.

Mohanraj Rajesh, et al. Biochem Biophys Res Commun. ;350(4):1056-1062.
3.
Fig.2

Fig.2. PARP inhibitors attenuate VEGF- and FGF-stimulated migration of HUVECs. From: Poly(ADP-ribose) polymerase inhibition decreases angiogenesis.

(A) Representative fields of cell culture inserts containing HUVECs migrated in response to the treatments as indicated.
(B) Quantitative data on the effect of IQD as indicated.
(C) Quantitative data on the effect of 5-AIQ as indicated.
* P < 0.00001 vs. control; # P < 0.05 vs.VEGF or FGF; ‡ P < 0.01 vs. VEGF or FGF; † P< 0.001 vs. VEGF or FGF, n = 3.

Mohanraj Rajesh, et al. Biochem Biophys Res Commun. ;350(4):1056-1062.
4.
Fig.3

Fig.3. PARP inhibitors attenuate VEGF- and FGF-induced tube formation of HUVECs. From: Poly(ADP-ribose) polymerase inhibition decreases angiogenesis.

HUVECs were treated with either VEGF/FGF or PARP inhibitors +VEGF/FGF and in vitro angiogenesis assays were performed using growth factor reduced Matrigel as described in the Methods section.
(A) Representative images of the tube formation of HUVECs when treated with treatments as indicated.
(B) Quantitative data on the effect of IQD on the tube formation of HUVECs.
(C) Quantitative data on the effect of 5-AIQ on the tube formation of HUVECs.
* P < 0.00001 vs. control; # P < 0.05 vs. VEGF or FGF; ‡ P < 0.01 vs. VEGF or FGF; † P< 0.001 vs. VEGF or FGF, n = 3.

Mohanraj Rajesh, et al. Biochem Biophys Res Commun. ;350(4):1056-1062.

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