We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 4

1.
<b>Figure 2</b>

Figure 2. From: Depletion of mitochondrial DNA in leucocytes harbouring the 3243A->G mtDNA mutation.

 Relationship between the percentage of the 3243A→G mitochondrial DNA (mtDNA) mutation in blood and age (years) for 11 subjects measured by last cycle fluorescent polymerase chain reaction (PCR). Each patient has a different symbol and colour, with serial measurements on the same patient connected by a straight line. PCR products were not diluted before loading.

Angela Pyle, et al. J Med Genet. 2007 January;44(1):69-74.
2.
<b>Figure 3</b>

Figure 3. From: Depletion of mitochondrial DNA in leucocytes harbouring the 3243A->G mtDNA mutation.

 Total number of mitochondrial DNA (mtDNA) molecules per cell in 10 controls and 11 subjects with the 3243A→G mutation. Serial measurements are represented by time points 1 and 2. Total mtDNA (mtDNA copy number) was determined by real‐time polymerase chain reaction (PCR) using iQ Sybr Green on the BioRad ICycler. Each data point represents the mean of three independent measurements from the original genomic DNA sample. Solid horizontal lines represent the mean value for each dataset.

Angela Pyle, et al. J Med Genet. 2007 January;44(1):69-74.
3.
<b>Figure 4</b>

Figure 4. From: Depletion of mitochondrial DNA in leucocytes harbouring the 3243A->G mtDNA mutation.

 Mean amount of wild‐type mitochondrial DNA (mtDNA) in 11 subjects with the 3243A→G mutation on two occasions. y Axis: wild‐type mtDNA (mtDNA copy number) was determined by real‐time polymerase chain reaction (PCR) in triplicate using iQ Sybr Green on the BioRad ICycler to determine the total amount of mtDNA. The wild‐type mtDNA was calculated from the mean percentage determined by last cycle fluorescent PCR shown in fig 2. x Axis: age of the subject (years) at the time of sampling. Each patient has a different symbol and colour, with the serial measurements connected by a straight line and using the same colour code as fig 2.

Angela Pyle, et al. J Med Genet. 2007 January;44(1):69-74.
4.
<b>Figure 1</b>

Figure 1. From: Depletion of mitochondrial DNA in leucocytes harbouring the 3243A->G mtDNA mutation.

 Comparison of the last cycle fluorescent polymerase chain reaction‐ restriction fragment length polymorphism (PCR‐RFLP) method with the last cycle hot PCR‐RFLP method to quantify the percentage of 3243A→G mutation in mixed clones (see Methods). y Axis: percentage of 3243A→G mutation determined from the relative fluorescence units (standard deviation (SD) from three independent measurements) of either the peak height amplitude (left‐hand graphs) or the area under the curve. x Axis: percentage of 3243A→G mutation determined by the last cycle hot PCR (SD from three independent measurements). Each pair of graphs shows the results from the serial dilution (from undiluted to 1:32 in water) of the last cycle fluorescent PCR‐RFLP products run independently on the fluorescent genetic analyser in triplicate. R2 = Spearman correlation coefficient using the individual data points (ie not the mean values).

Angela Pyle, et al. J Med Genet. 2007 January;44(1):69-74.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk