Results: 3

1.
Fig. 1

Fig. 1. From: Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two-dimensional scanning mirror.

Electron micrographs of a two-dimensional MEMS scanner. a, 750 μm × 750 μm scanning mirror in a 3.2 mm × 3.0 mm die. Six banks of vertical comb actuators drive the mirror, which has a gimbal design. b, Inner axis torsional spring. c, Outer axis comb bank. Scale bars are 250 μm.

Wibool Piyawattanametha, et al. Opt Lett. ;31(13):2018.
2.
Fig. 2

Fig. 2. From: Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two-dimensional scanning mirror.

(Color online) Response characteristics of a 750 μm × 750 μm MEMS scanner. For both a and b, the voltage signal was applied to only one of the two opposing comb banks for each rotational axis. a, Optical deflection angle as a function of dc voltage. The maximum deflection angles are ±7.6° and ±3.0° for the inner (blue solid curve) and outer (red dashed curve) axes, respectively. b, Frequency response functions for the inner (blue solid curve) and outer (red dashed curve) axes, obtained by applying voltage signals of peak-to-peak amplitudes 45 and 58 V to the inner and outer axes, respectively.

Wibool Piyawattanametha, et al. Opt Lett. ;31(13):2018.
3.
Fig. 3

Fig. 3. From: Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two-dimensional scanning mirror.

Two-photon fluorescence images of pollen grains acquired using instrumentation based on a MEMS scanner and a Ti:sapphire laser tuned to 850 nm. a–c, Images acquired using a 40× 0.8 NA water-immersion microscope objective. The fast-axis acquisition rate was 3.2 kHz for a and b and 3.0 kHz for c. Image b is a sum projection from a stack of 38 images acquired at 1 μm increments. d and e, Images acquired using a doublet GRIN microendoscope probe of 0.47 NA and a 10× 0.25 NA microscope objective to couple light into the probe.3,5 The fast axis was driven at resonance, allowing a double-sided acquisition rate of 3.52 kHz. Image e is a maximum intensity projection from a stack of 46 images acquired at 1 μm increments. Laser power at the sample was 20 mW for a and b, 28 mW for c, and 40 mW for d and e. These power levels were needed because of the fast acquisition rate. Scale bars are 5 μm.

Wibool Piyawattanametha, et al. Opt Lett. ;31(13):2018.

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