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Results: 5

1.
Fig. 5.

Fig. 5. From: Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis.

Evolutionary analysis. Phylogenetic tree for Cc’s from various species, constructed as described in Materials and Methods. The clades for testicular and somatic Cc’s are marked pink and green, respectively.

Zhe Liu, et al. Proc Natl Acad Sci U S A. 2006 June 13;103(24):8965-8970.
2.
Fig. 4.

Fig. 4. From: Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis.

The region around the Arg-38 site in T-Cc (A) and hh-Cc (B). Water molecules are shown as cyan spheres. Possible hydrogen bond interactions are marked as black dashed lines. The ferric ion in the center of the heme is shown as an orange sphere. The carbon, oxygen, and nitrogen atoms are in yellow, red, and blue, respectively.

Zhe Liu, et al. Proc Natl Acad Sci U S A. 2006 June 13;103(24):8965-8970.
3.
Fig. 1.

Fig. 1. From: Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis.

Schematic diagram of T-Cc illustrated from the proximal side, as viewed from the heme opening of the molecule. Backbones are drawn with the ribbon model and colored white. The 14 residues different to those in S-Cc are shown in the ball and stick model and colored orange. Five water molecules located in the internal part of the protein are in cyan.

Zhe Liu, et al. Proc Natl Acad Sci U S A. 2006 June 13;103(24):8965-8970.
4.
Fig. 3.

Fig. 3. From: Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis.

Apoptotic activity of T-Cc. (A) Effect of T-Cc and S-Cc on the rate of DEVD-pNA cleavage. Initial rates of DEVD-pNA cleavage were determined by comparing to the standard curve for pNA. (B) Chromatin condensation induced by adding 1 μM T-Cc and erythrocyte nuclei into Xenopus egg extracts. Samples were fixed and stained by DAPI at the time indicated for fluorescence observation. (C) DNA fragmentation assays were carried out by adding various concentrations of T-Cc, S-Cc, and 105 chicken erythrocyte nuclei into Xenopus egg extracts, followed by electrophoresis (see Materials and Methods).

Zhe Liu, et al. Proc Natl Acad Sci U S A. 2006 June 13;103(24):8965-8970.
5.
Fig. 2.

Fig. 2. From: Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis.

Kinetic studies of T-Cc. (A) The initial rates of H2O2 reduction by various concentrations of ferroT-Cc, ferroS-Cc, and ferrohh-Cc. (B) Degradation of the heme of ferriT-Cc, ferriS-Cc, and ferrihh-Cc in the presence of 3 mM H2O2. (C) Heme destruction of ferriT-Cc as indicated by dissipation of the Soret band measured at 408 nm. Spectra were scanned every 3 min. (D) Time course of ferroT-Cc oxidation (solid line) and inactivation (dashed line) in the presence of 3 mM H2O2. (E) Reduction titration of T-Cc, S-Cc, and hh-Cc with ascorbate. (F) Activities of purified CcO with T-Cc and S-Cc. Measurements were performed under both low pH (50 mM phosphate/Tris, pH 6.5) and high pH (25 mM acetate/Tris, pH 7.8) conditions.

Zhe Liu, et al. Proc Natl Acad Sci U S A. 2006 June 13;103(24):8965-8970.

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