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Results: 5

1.
Figure 5

Figure 5. From: Defining the Human Macula Transcriptome and Candidate Retinal Disease Genes UsingEyeSAGE.

Real-time qRT-PCR summaries of rod-specific (PDE6A), RPE-specific (RDH5), and candidate RPE (EMP3 and MMP25) gene expression in the peripheral retina, RPE/choroid, and RPE cells (RPE-enriched) relative to expression in the peripheral retina.

Catherine Bowes Rickman, et al. Invest Ophthalmol Vis Sci. ;47(6):2305.
2.
Figure 2

Figure 2. From: Defining the Human Macula Transcriptome and Candidate Retinal Disease Genes UsingEyeSAGE.

Real-time qRT-PCR summaries of known rod-specific (PDE6A), cone-specific (PDE6C and GNAT2), ganglion cell-associated (THY-1), and candidate cone photoreceptor–associated genes (SH3BGRL2, CPLX4, DIRAS2, HR, KIAA1345, ZNF595OS, and UCHL1) in the macula relative to expression in the peripheral retina.

Catherine Bowes Rickman, et al. Invest Ophthalmol Vis Sci. ;47(6):2305.
3.
Figure 3

Figure 3. From: Defining the Human Macula Transcriptome and Candidate Retinal Disease Genes UsingEyeSAGE.

Real-time qRT-PCR summaries of cone-specific (PDE6C and GNAT2), candidate cone photoreceptor (HR, CPLX4, and KIAA1345) and ganglion cell-associated (THY-1) gene expression in regions of the human retina relative to expression in peripheral retina (calculated using the comparative CT method). PR, photoreceptor layer; Peri, peripheral.

Catherine Bowes Rickman, et al. Invest Ophthalmol Vis Sci. ;47(6):2305.
4.
Figure 1

Figure 1. From: Defining the Human Macula Transcriptome and Candidate Retinal Disease Genes UsingEyeSAGE.

Posterior eyecup of a human donor eye. Right eye obtained from a 64-year-old white male donor within 4 hours of death and stored in RNA preservative. The donor had no ocular history of disease. Macular (M) and midperipheral (P) regions, from which 4-mm diameter punches were taken.

Catherine Bowes Rickman, et al. Invest Ophthalmol Vis Sci. ;47(6):2305.
5.
Figure 4

Figure 4. From: Defining the Human Macula Transcriptome and Candidate Retinal Disease Genes UsingEyeSAGE.

Single human photoreceptor (A) isolation and (B) RT-PCR. (A) Single photoreceptor cells were aspirated into micropipette. (B) Ethidium bromide–stained agarose gel of PCR products amplified in nested RT-PCR reactions of single photoreceptor cDNAs. Rod, rodspecific PDE6A gene primers (124-bp product) plus UCHL1 primers (153-bp product) or plus SH3BGRL2 primers (100-bp product); “cone” cone-specific PDE6C gene primers (195-bp product) plus UCHL1 primers or SH3BGRL2 primers. UCHL1 is expressed in the inner retina and is therefore not detected in either photoreceptor derived cDNA pool. Lanes 1, 2: UCHL1 reactions; lane 3: 100-bp ladder; lanes 4, 5: SH3BGRL2 reactions.

Catherine Bowes Rickman, et al. Invest Ophthalmol Vis Sci. ;47(6):2305.

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