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Results: 5

1.
Fig. 3.

Fig. 3. From: Antibodies to native myelin oligodendrocyte glycoprotein are serologic markers of early inflammation in multiple sclerosis.

Analysis of human serum IgG reactivity against hMOGcme. BR normalized (BRN) is the Gmean value of IgG binding to MOG-transfected CHO cells divided by that of IgG binding to ntCHO cells, normalized to the value of the positive control tested in each plate. Horizontal bars = median. See text for details.

Patrice H. Lalive, et al. Proc Natl Acad Sci U S A. 2006 February 14;103(7):2280-2285.
2.
Fig. 2.

Fig. 2. From: Antibodies to native myelin oligodendrocyte glycoprotein are serologic markers of early inflammation in multiple sclerosis.

Binding characteristics of MOG-specific marmoset recombinant Fab fragments. Binding of monoclonal Fabs specific for rMOG125 (0.5 μg/ml) by FACS against hMOGcme. Background is represented by the binding to the ntCHO cells (open traces) and compared with the binding to MOG-transfected CHO cells (filled traces). Both Fabs M26 (A) and M3-31 (B) strongly recognize an epitope displayed on hMOGcme, in contrast to Fabs M3-24 (C) and M3-8 (D).

Patrice H. Lalive, et al. Proc Natl Acad Sci U S A. 2006 February 14;103(7):2280-2285.
3.
Fig. 4.

Fig. 4. From: Antibodies to native myelin oligodendrocyte glycoprotein are serologic markers of early inflammation in multiple sclerosis.

Time course of serum IgG directed against hMOGcme in marmoset EAE. Results are from 11 EAE C. jacchus marmosets immunized with human white matter, 3 of which were killed before onset of clinical disease. First occurrence of serum IgG directed against hMOGcme is compared with time of clinical onset of EAE in a Kaplan–Meier survival plot. Please see text and Table 1 for details.

Patrice H. Lalive, et al. Proc Natl Acad Sci U S A. 2006 February 14;103(7):2280-2285.
4.
Fig. 1.

Fig. 1. From: Antibodies to native myelin oligodendrocyte glycoprotein are serologic markers of early inflammation in multiple sclerosis.

Cell-based (hMOGcme) assay. (A and B) Staining of MOG-transfected CHO cells with anti-MOG Ab 8-18C5 (0.5 μg/ml) and detection by FACS (A) and immunofluorescence (B). (B Inset) Negative control omitting primary Ab. (C) Positive control patient serum (RRMS 1158, 1:10) displaying a clear shift for MOG-transfected CHO cells (filled trace) when compared with ntCHO cells (open trace). (D) Mean BR (BR ± SEM) calculated as the Gmean from nine independent assays.

Patrice H. Lalive, et al. Proc Natl Acad Sci U S A. 2006 February 14;103(7):2280-2285.
5.
Fig. 5.

Fig. 5. From: Antibodies to native myelin oligodendrocyte glycoprotein are serologic markers of early inflammation in multiple sclerosis.

Selective epitope presentation on hMOGcme. (A) Binding to hMOG125 by ELISA compared with binding to hMOGcme by FACS in the CIS cohort (n = 36). By linear regression analysis, there is no correlation between the results of these two methods (P not significant, r2 = 0.00023, Spearman r; straight line is the linear regression curve; dotted line indicates 95% confidence interval) even when clear serum reactivity is present in both assays. BRN, BR normalized. (B and C) Preabsorption of serum on either hMOGcme (Left) or hMOG125 (Right), followed by testing by FACS (B, hMOGcme) or ELISA (C, hMOG125). Preabsorption on hMOG125 or hMOGcme only altered the reactivity in the corresponding system of detection. See Materials and Methods for additional details.

Patrice H. Lalive, et al. Proc Natl Acad Sci U S A. 2006 February 14;103(7):2280-2285.

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