Results: 4

1.
Fig. 4.

Fig. 4. From: PTEN negatively regulates neural stem cell self-renewal by modulating G0-G1 cell cycle entry.

Gene expression analysis. (A) Microarray data can be classified into two major groups, genes that are up-regulated in Pten null neurosphere cultures (upper box) and genes that are down-regulated in Pten null neurosphere cultures (lower box). (B) Gene Ontology analysis.

Matthias Groszer, et al. Proc Natl Acad Sci U S A. 2006 January 3;103(1):111-116.
2.
Fig. 2.

Fig. 2. From: PTEN negatively regulates neural stem cell self-renewal by modulating G0-G1 cell cycle entry.

Pten null neurospheres are hypersensitive to growth factor stimulation. Identical number of cells from WT and MUT E14.5 front brains were seeded in neurosphere cultures with the indicated concentration of FGF2. Neurosphere numbers (A) and size (B) were measured 7 days after initial culture and presented as mean ± SD.

Matthias Groszer, et al. Proc Natl Acad Sci U S A. 2006 January 3;103(1):111-116.
3.
Fig. 1.

Fig. 1. From: PTEN negatively regulates neural stem cell self-renewal by modulating G0-G1 cell cycle entry.

PTEN loss leads to greater and persistent self-renewal capacity. (A) A schematic illustration of the serial neurosphere culture system. (B) Percentages of WT and MUT sphere cultures that can be serially passaged. (C) Sphere formation relative to WT sphere counts at each passage. (D) Histograms show the neurogenic potential of WT (in blue) and MUT (in red) neurosphere cultures. WT spheres demonstrated a loss of neurogenic potential over time with serial, low-density passages. Filled bars, spheres with neuron; hatched bars, spheres without neuron. (E) Representative images of the neurogenic potential of control and mutant neurospheres, with propridium iodide staining in Left and TuJ1 immunostaining in Right. (Scale bar: 17 μm.) A, GFAP+ astrocytes; O, O4+ oligodendrocytes; N, Tuj1+ neurons.

Matthias Groszer, et al. Proc Natl Acad Sci U S A. 2006 January 3;103(1):111-116.
4.
Fig. 3.

Fig. 3. From: PTEN negatively regulates neural stem cell self-renewal by modulating G0-G1 cell cycle entry.

Loss of PTEN leads to increased G0/G1 A to G1B cell cycle transition as well as increased cell growth. (A) Flowcytometric analysis of cell cycle status in primary cells from E14.5 cortices (Upper) and cells that have gone through one generation of neurosphere culture (Lower). Left, WT; Right, MUT. N-butyrate treated neurosphere cultures, which are arrested at the G1 A to G1B transition, were used to demarcate the line (vertical axes) between cells falling into the G0/G1A stage (quiescence, Lower Left) and cells which have entered the G1B stage of the cell cycle (Lower Right). (B Left) A comparison of cell cycle profiles. Statistic analysis for brain: G0/G1A, P = 0.05; G1B, P = 0.08; S/G2/M, P = 0.43; neurosphere: G0/G1A, P = 0.05; G1B, P = 0.72; S/G2/M, P = 0.00003. *, statistically significant. (Right) Cell size profiles at different cell cycle stages. Data include all experiments conducted. For brain, WT, n = 10; Mut, n = 5; for neurosphere, WT, n = 11; Mut, n = 7.

Matthias Groszer, et al. Proc Natl Acad Sci U S A. 2006 January 3;103(1):111-116.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk