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Results: 5

1.
Fig. 4.

Fig. 4. From: Activation-induced deaminase cloning, localization, and protein extraction from young VH-mutant rabbit appendix.

Double staining with anti-RAID in red, anti-UNG (AD) in green, and DAPI in blue on cytospin slides of sorted EMA-negative cells from 4.5-wk-old ali/ali appendix cell suspension. (Scale bar: 5 μm.)

Guibin Yang, et al. Proc Natl Acad Sci U S A. 2005 November 22;102(47):17083-17088.
2.
Fig. 3.

Fig. 3. From: Activation-induced deaminase cloning, localization, and protein extraction from young VH-mutant rabbit appendix.

Seven-micrometer 4.5-wk-old ali/ali appendix sections were stained with Alexa Fluor 568-anti-RAID (red), FITC-anti-IgM (green), Alexa Fluor 647-anti-macrophage (white), and DAPI (blue). (A) Whole follicle. (BD) High-magnification images captured from A in the order from top to bottom. Double staining with anti-RAID in red, anti-IgM (E and F), anti-IgA (G and H), or anti-macrophage (IK) in green, and DAPI in blue on cytospin slides from 4.5-wk-old ali/ali appendix cell suspension. (Scale bar: 5 μm.)

Guibin Yang, et al. Proc Natl Acad Sci U S A. 2005 November 22;102(47):17083-17088.
3.
Fig. 1.

Fig. 1. From: Activation-induced deaminase cloning, localization, and protein extraction from young VH-mutant rabbit appendix.

Rabbit AID sequence and comparisons with other species. (A) DNA sequence and deduced encoded protein sequence of rabbit AID. Lowercase letters at the 5′ end indicate the location of the primer sequence used for initial PCR amplification. (B) Rabbit AID amino acid sequence compared with human, mouse, and chicken AID sequences. The red rectangles represent the locations of peptides synthesized for use as immunogens.

Guibin Yang, et al. Proc Natl Acad Sci U S A. 2005 November 22;102(47):17083-17088.
4.
Fig. 5.

Fig. 5. From: Activation-induced deaminase cloning, localization, and protein extraction from young VH-mutant rabbit appendix.

Cell cycle and detection of AID. (A) Flow cytometry of 4.5-wk-old ali/ali appendix cells stained with anti-IgM and anti-AID or rabbit IgG isotype control. (B) Cytospin slides of sorted EMA-negative cells from 4.5-wk-old ali/ali appendix stained by anti-Ki-67 and anti-RAID. (C) FACS analysis of AID and cell division cycle. Data shown represent one of three analyses. (D) Double staining on cytospin slides of sorted EMA-negative cells from 4.5-wk-old ali/ali appendix with anti-RAID and anti-phospho-Histone 3 (HAT28).

Guibin Yang, et al. Proc Natl Acad Sci U S A. 2005 November 22;102(47):17083-17088.
5.
Fig. 2.

Fig. 2. From: Activation-induced deaminase cloning, localization, and protein extraction from young VH-mutant rabbit appendix.

Results of Western blotting of nuclear and cytoplasmic extracts from 4.5-wk-old ali/ali appendix. (A) Twenty-five, 50, 100, and 200 μg of total protein from cytoplasmic and nuclear extracts were loaded per well. Recombinant mouse AID expressed in E. coli dissolved in 8 M urea was the positive control (PC). Blots were probed with anti-RAID, anti-HAID, or normal rabbit IgG (exposed for 30 min) and anti-β-actin (exposed for 5 min). Results of blocking with HAID or RAID peptide followed by probing with anti-RAID Ab are also shown. (B) Partially purified protein from two 4.5-wk-old ali/ali appendixes was loaded on the gel stained with Coomassie blue (B) or silver (C). The blots were probed with anti-RAID Ab, and corresponding bands (indicated with arrows) were cut from the gels and analyzed by MS to identify the AID sequence. (D) Peptide sequences of rabbit AID detected by MS. PC, positive control; T, total extract; P, purified; M, molecular weight marker.

Guibin Yang, et al. Proc Natl Acad Sci U S A. 2005 November 22;102(47):17083-17088.

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