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Results: 4

1.
Figure 4

Figure 4. DC-SIGN Mediates M. tuberculosis Binding to Alveolar Mφs from Patients with TB. From: DC-SIGN Induction in Alveolar Macrophages Defines Privileged Target Host Cells for Mycobacteria in Patients with Tuberculosis.

(A) Alveolar Mφs from a patient with TB were infected with GFP-expressing M. tuberculosis, in the absence (ø; upper left panel) or the presence of control isotype (upper right panel), anti-CD11b (lower left panel), or -DC-SIGN (lower right panel) blocking antibodies. In the upper panels, cells were then stained with fluorescent PE-conjugated anti-DC-SIGN and APC-conjugated anti-CD11b antibodies. In lower panels, fluorescent antibodies were added together with blocking antibodies (same clones).
(B) Proportion of GFP+ cells in DC-SIGN (open bars) and DC-SIGN+ (grey bars) alveolar Mφs as calculated from (A) using BALs from two patients with TB. THP1 Mφs expressing or not expressing DC-SIGN (THP1::DC-SIGN) were used in a binding experiment with M. tuberculosis H37Rv, in the presence or absence of anti-DC-SIGN antibodies.
(D) Confocal microscopy examination of adherent DC-SIGN+ cells infected with GFP-expressing M. tuberculosis for various times.

Ludovic Tailleux, et al. PLoS Med. 2005 December;2(12):e381.
2.
Figure 2

Figure 2. Alveolar DC-SIGN+ Cells in Patients with TB Are Mφs. From: DC-SIGN Induction in Alveolar Macrophages Defines Privileged Target Host Cells for Mycobacteria in Patients with Tuberculosis.

(A) Total BAL cells from a patient with TB were allowed to adhere to the plastic for 1 h at 37 °C in complete medium. CD11b and DC-SIGN expression was analyzed by flow cytometry before (left) and after (right) adherence.
(B) Surface and intracellular DC-SIGN (red) expression by an adherent alveolar cell examined under the confocal microscope.
(C) Flow cytometry analysis of surface expression of BDCA-1 (CD1c), BDCA-2, BDAC-3, CD1a, CD11b, CD11c, CD14, CD68, CD83, and CD123 in DC-SIGN+ BAL cells from a patient with TB.
(D) Flow cytometry analysis of surface expression of CD40, CD86, HLA-DR, CD11b, CD11c, CD206, CD16, CD32, CD40, CD64, TLR2, TLR4, and TLR9 in DC-SIGN+ BAL cells from a patient with TB.
In (C) and (D), analysis was performed on DC-SIGN–expressing cells in R2, as shown in Figure 1.

Ludovic Tailleux, et al. PLoS Med. 2005 December;2(12):e381.
3.
Figure 1

Figure 1. Alveolar CD11b+ Cells Over-Express DC-SIGN in Patients with TB. From: DC-SIGN Induction in Alveolar Macrophages Defines Privileged Target Host Cells for Mycobacteria in Patients with Tuberculosis.

(A) BAL cells from a patient with TB (upper four panels) and from a patient with sarcoidosis (lower two panels) were analyzed by flow cytometry. Expression of CD3 and CD4 was analyzed on cells from R1. CD11b and DC-SIGN expression was analyzed on cells from R2.
(B) Distribution of the proportion of CD11b+DC-SIGN+ cells in BALs according to pathology and age. Black circles indicate ≤15 y of age; black triangles indicate ≥20 y; NC, no case.
(C) DC-SIGN (upper panels) and M. tuberculosis (lower panels) immunodetection in serial sections of a lung biopsy from a patient with TB. The pictures are representative of results obtained with samples from a total of four patients. G, granuloma.
(D) DC-SIGN immunodetection in a lung biopsy from a patient with sarcoidosis. The pictures are representative of results obtained with samples from a total of three patients.
In (C) and (D), magnification in left panels is 100×, and regions in squares are shown at higher magnification in right panels.

Ludovic Tailleux, et al. PLoS Med. 2005 December;2(12):e381.
4.
Figure 3

Figure 3. DC-SIGN Is Induced on Resident Alveolar Mφs upon M. tuberculosis Infection. From: DC-SIGN Induction in Alveolar Macrophages Defines Privileged Target Host Cells for Mycobacteria in Patients with Tuberculosis.

(A) Flow cytometry analysis of CD11b and DC-SIGN expression by PBMCs from a healthy donor (upper panels) and a patient with TB (lower panels).
(B) Adherent DC-SIGN alveolar Mφs from a non-tuberculous patient were infected with a GFP-expressing strain of M. tuberculosis at a MOI of one bacterium per cell, or treated with IL-4, TNF-α, or LPS, or left untreated (ø control). After 48 h at 37 °C, cells were recovered and DC-SIGN expression was analyzed by flow cytometry. In M. tuberculosis panel, the grey area corresponds to GFP+ (infected) cells, and the plain line corresponds to GFP (uninfected) cells.
(C) Cells infected with M. tuberculosis at a MOI of 1 for 1, 2, 4, or 24 h were analysed by RT-PCR for DC-SIGN and GAPDH mRNAs.

Ludovic Tailleux, et al. PLoS Med. 2005 December;2(12):e381.

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