Results: 4

1.
Figure 2.

Figure 2. From: FGF-2 binding to fibrin(ogen) is required for augmented angiogenesis.

Effect of FGF-2 mutants on new vessel formation in a placental explant model. The growth of vessels from human placental vessel fragments cultured in a fibrin gel with Medium 199 and 10% FBS for 18 days, in the presence or absence of FGF-2 or FGF-2 mutants is shown. (A) Control; (B) wtFGF-2; (C) fibrinogen nonbinding mutant (2212); (D) fibrinogen-binding mutant (221*2). Right panel: New vessel growth was quantified using image analysis software. Both wtFGF-2 and 221*2 supported significantly increased vessel growth over medium alone (P < .05 for both). The results are the mean ± SE of 3 separate experiments.

Abha Sahni, et al. Blood. 2006 January 1;107(1):126-131.
2.
Figure 4.

Figure 4. From: FGF-2 binding to fibrin(ogen) is required for augmented angiogenesis.

Effect of fibrinogen on FGF-2-induced angiogenesis in Matrigel plugs in C57-BL6 mice. Matrigel supplemented with PBS (A), fibrinogen (500 μg/mL) (B), wtFGF-2 (500 ng/mL) (C), FGF-2 and fibrinogen (D), 2212 (E), 2212 plus fibrinogen (F), 221*2 (G), or 221*2 plus fibrinogen (H) was injected subcutaneously into C57BL/6 female mice. After 10 days, the Matrigel plugs were removed, fixed in 3% formalin, embedded in paraffin, sectioned, and stained with trichrome. Bar represents 100 μm. Top right panel: vessel growth was quantitated by counting the number of new vessels and branches and using image analysis software. Ten mice were used in each group. Data represent mean ± SEM. Bottom right panel: The hemoglobin content in Matrigel plugs removed at 10 days was measured using Drabkin reagent. Hemoglobin reflects the amount of blood in the plugs and provides an estimate of the amount of vascularization. Results are shown as mean ± SE.

Abha Sahni, et al. Blood. 2006 January 1;107(1):126-131.
3.
Figure 1.

Figure 1. From: FGF-2 binding to fibrin(ogen) is required for augmented angiogenesis.

EC proliferation in the presence of FGF-2 mutants. (A) ECs were plated on gelatin-coated wells in McCoy 5A medium supplemented with 20% FBS, 50 μg/mL ECGS, and 100 μg/mL heparin and allowed to adhere for 6 hours. The cells were then washed twice with McCoy medium and incubated in serum-free medium containing 1% Nutridoma, 1 μCi (0.037 MBq) 3H-thymidine with 25 ng/mL of wtFGF-2 or FGF-2 mutants (2212 or 221*2) in the presence or absence of 100 μg/mL fibrinogen (FBG) for 24 hours. Isotope incorporated into DNA was precipitated with TCA, collected by vacuum filtration, and measured by scintillation counting. (B) To characterize specificity, the same experiment was conducted comparing the response with 100 μg/mL fibrinogen, gelatin (GEL), or vitronectin (VN). Neither gelatin nor vitronectin significantly increased the response to FGF-2. Results are the mean ± SD of 3 different experiments.

Abha Sahni, et al. Blood. 2006 January 1;107(1):126-131.
4.
Figure 3.

Figure 3. From: FGF-2 binding to fibrin(ogen) is required for augmented angiogenesis.

Effect of fibrinogen, FGF-2, and FGF-2 mutants on new vessel formation in the chicken CAM model. Filter discs soaked in PBS (A), fibrinogen (FBG) (B), FGF-2 (C), non-fibrinogen-binding FGF-2 mutant 2212 (D), fibrinogen-binding FGF-2 mutant 221*2 (E), 2212 plus FBG (F), 221*2 plus FBG (G), and wtFGF-2 plus FBG (H). FGF-2 (200 ng/disc) and FBG (20 μg/disc) in a total volume of 20 μL were applied on 8-day-old CAMs. After 72 hours of incubation at 37°C, filters were removed, and each CAM was fixed and photographed. A CAM with a filter disc containing PBS without growth factors was used as control. Bar represents 100 μm. Right column: Quantitation of new vessel formation in the chicken CAM model. The number of new vessels and branches (top) and the average length of new vessels (bottom) were quantified using image analysis software. Seven embryos in each group were used. Data represent mean ± SE.

Abha Sahni, et al. Blood. 2006 January 1;107(1):126-131.

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