Results: 5

1.
FIG. 1.

FIG. 1. From: An Attenuated LC16m8 Smallpox Vaccine: Analysis of Full-Genome Sequence and Induction of Immune Protection .

ORF map of the LC16m8 and LC16mO strains. The ORFs transcribed rightward and leftward are presented above and below the horizontal centerlines, respectively. The major difference between the two strains is boxed. Putative functions of ORFs were evaluated or predicted by a BLAST search of the GenBank database and are expressed in different colors. The double-headed arrows indicate the regions of the ITRs of the left and right ends.

Shigeru Morikawa, et al. J Virol. 2005 September;79(18):11873-11891.
2.
FIG. 2.

FIG. 2. From: An Attenuated LC16m8 Smallpox Vaccine: Analysis of Full-Genome Sequence and Induction of Immune Protection .

Differences in nucleotide sequences between the LC16m8 and LC16mO strains. (A) The locations (1 to 6) of nucleotide point mutations in the genomes are shown schematically. (B) The nucleotide changes are shown in boldface lowercase letters. The resultant amino acid changes in ORFs are indicated by shaded boldface italics in loci (2, 5, and 6). Putative gene functions and the ORFs corresponding to the CPN strain are also shown.

Shigeru Morikawa, et al. J Virol. 2005 September;79(18):11873-11891.
3.
FIG. 4.

FIG. 4. From: An Attenuated LC16m8 Smallpox Vaccine: Analysis of Full-Genome Sequence and Induction of Immune Protection .

Comparison of amino acid alignments of the EEV Env-related proteins in six vaccinia virus strains and other OPVs. The numbers at the top of each panel indicate the amino acid positions of the EEV proteins of vaccinia virus CPN strain. The asterisks and dashes show conserved and deleted amino acids, respectively, with reference to CPN. The vaccinia viruses compared are CPN, Lister (calf lymph Lister vaccine), LC16mO, WR, IHD-J, and MVA strains. Variola, monkeypox, and cowpox viruses shown for reference are Bangladesh-1975, Zaire-96-I-16, and GRI-90 strains, respectively.

Shigeru Morikawa, et al. J Virol. 2005 September;79(18):11873-11891.
4.
FIG. 3.

FIG. 3. From: An Attenuated LC16m8 Smallpox Vaccine: Analysis of Full-Genome Sequence and Induction of Immune Protection .

Polymorphism of the Lister strain genome. (A) Nucleotide sequence variations are presented in each 500-bp length along the central coding region of the Lister genome. (B) Six divergent loci, L0202, L0403, L0638, L0640, L1000, and L1100, were randomly selected. LO and mO genomic DNAs were amplified at the selected sites by PCR with the forward primers specific for LO or mO and the common reverse primers. (C) The marginal (150-bp) region spanning diversity numbers L1121 to -1124 of LO virus DNA were cloned, sequenced, and classified into eight genotypes. The genotype of LC16mO is also shown.

Shigeru Morikawa, et al. J Virol. 2005 September;79(18):11873-11891.
5.
FIG. 5.

FIG. 5. From: An Attenuated LC16m8 Smallpox Vaccine: Analysis of Full-Genome Sequence and Induction of Immune Protection .

Protection against lethal WR challenge by vaccination with LC16m8. Groups of 6-week-old BALB/c mice were subcutaneously vaccinated and intranasally challenged as for Table 2. (A) Levels of antibodies in pre- and postchallenge sera of individual mice. Sera were examined by ELISA for vaccinia virus- and B5R-specific antibodies, and the results are shown with OD405 values at 1:400 and 1:100 dilutions, respectively. The horizontal bars indicate the averages. (B) Histopathology by HE staining and IHC by peroxidase staining of the nasal tissue collected from nonimmunized and vaccinated mice 9 and 14 days after challenge infection, respectively. (C) Survival and (D) bodyweights of mice after WR challenge. The mice had been vaccinated with 105 (open symbols) or 107 (solid symbols) PFU of LC16m8 (□ and ▪), LC16mO (○ and •), or Lister (▵ and ▴) strain or PBS (⧫). To avoid confusion, the average bodyweight ± standard deviation is shown in separate panels in comparison with the PBS group. The crosses indicate the deaths of mice.

Shigeru Morikawa, et al. J Virol. 2005 September;79(18):11873-11891.

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